Name: Recombinant Human FLT4 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-2115
Availability: InStock

Recombinant Human FLT4 Protein (His Tag)

Catalog No.: BLPSN-2115

Product Overview

Tag	His
Host Species	Human
Accession	NP_002011.2
Synonym	FLT-4, FLT-41, FLT41, LMPH1A, PCL, VEGF Receptor 3, VEGFR-3, VEGFR3
Background	Vascular endothelial growth factor receptor 3 (VEGFR3), also known as FLT-4, together with the other two members VEGFR1 (FLT-1) and VEGFR2 (KDR/Flk-1) are receptors for vascular endothelial growth factors (VEGF) and belong to the class III subfamily of receptor tyrosine kinases (RTKs). The VEGFR3 protein is expressed mainly on lymphatic vessels but it is also up-regulated in tumor angiogenesis. Mutations in VEGFR3 have been identified in patients with primary lymphoedema. The VEGF-C/VEGF-D/VEGFR3 signaling pathway may provide a target for antilymphangiogenic therapy in prostate cancer, breast cancer, gastric cancer, lung cancer, non-small cell lung cancer (NSCLC), and so on.Immune CheckpointImmunotherapyCancer ImmunotherapyTargeted Therapy
Description	A DNA sequence encoding the extracellular domain (Met 1-Ile 776) of human VEGFR3 (NP_002011.2) was expressed with a C-terminal His tag.
Source	HEK293
Predicted N Terminal	Tyr 25 & Ser 473
AA Sequence	Met 1-Ile 776
Molecular Weight	The recombinant human VEGF R3 consists of 763 a.a. and predicts a molecular mass of 86 kDa. As a result of glycosylation, rhVEGFR3 migrates as an approximately 130 kDa in non-reduced SDS-PAGE.
Purity	>97% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	1. Measured by its binding ability in a functional ELISA.2. Immobilized human VEGF-C at 10 ug/mL (100 ul/well) can bind human VEGFR3-his. The EC50 of human VEGFR3-his is 0.011 ug/mL.3. Measured by its ability to bind human VEGF-D in a functional ELISA.
Formulation	Lyophilized from sterile PBS, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.