Name: Recombinant Human FLT4 Protein (Fc Tag)
Brand: Beta LifeScience
SKU: BLPSN-2114
Availability: InStock

Catalog No.: BLPSN-2114

Product Overview

Tag	Fc
Host Species	Human
Accession	NP_002011.2
Synonym	FLT-4, FLT-41, FLT41, LMPH1A, PCL, VEGF Receptor 3, VEGFR-3, VEGFR3
Background	Vascular endothelial growth factor receptor 3 (VEGFR3), also known as FLT-4, together with the other two members VEGFR1 (FLT-1) and VEGFR2 (KDR/Flk-1) are receptors for vascular endothelial growth factors (VEGF) and belong to the class III subfamily of receptor tyrosine kinases (RTKs). The VEGFR3 protein is expressed mainly on lymphatic vessels but it is also up-regulated in tumor angiogenesis. Mutations in VEGFR3 have been identified in patients with primary lymphoedema. The VEGF-C/VEGF-D/VEGFR3 signaling pathway may provide a target for antilymphangiogenic therapy in prostate cancer, breast cancer, gastric cancer, lung cancer, non-small cell lung cancer (NSCLC), and so on.Immune CheckpointImmunotherapyCancer ImmunotherapyTargeted Therapy
Description	A DNA sequence encoding the extracellular domain (Met 1-Ile 776) of human VEGFR3 (NP_002011.2) was expressed with the fused Fc region of human IgG1 at the C-terminus.
Source	HEK293
Predicted N Terminal	Tyr 25 & Ser 473
AA Sequence	NP_002011.2
Molecular Weight	The recombinant human VEGFR3/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 990 a.a. and predicts a molecular mass of 111 kDa. As a result of glycosylation and proteolytic cleavage, rhVEGFR3/Fc migrates as three bands (160, 85, 75 kDa) corresponding to the full length and the cleaved two polypeptides respectively in SDS-PAGE under reducing conditions.
Purity	>97% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Measured by its binding ability in a functional ELISA.1. Immobilized recombinant human VEGFR3 at 5 ug/ml (100 ul/well) can bind recombinant human VEGF-D at a linear range of 62.5-2000 ng/ml.2. Immobilized recombinant human VEGF-C at 10 ug/ml (100 ul/well) can bind recombinant human VEGFR3 at a linear range of 0.64-80 ng/ml.3. Scatchard analysis showed the affinity constant (Kd) of recombinant human VEGF-C bound to recombinant human VEGFR3 was 1.4 nM.
Formulation	Lyophilized from sterile PBS, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.