Name: Recombinant Human FLRT2 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-2102
Availability: InStock

Recombinant Human FLRT2 Protein (His Tag)

Catalog No.: BLPSN-2102

Product Overview

Tag	His
Host Species	Human
Accession	O43155
Synonym	KIAA0405, UNQ232/PRO265
Background	Fibronectin Leucine-Rich Transmembrane (FLRT) proteins are glycosylated membrane proteins expressed at the cell surface which localise in a homophilic manner to cell-cell contacts expressing the focal adhesion marker vinculin. FLRT1, FLRT2, and FLRT3, the three genes encode putative type I transmembrane proteins, each containing 1 leucine-rich repeats (LRR), a type III fibronectin (FN) domain, followed by the transmembrane region, and a short cytoplasmic tail. FLRT family members may function in cell adhesion and/or receptor signalling. Each member of the FLRT family has a distinct, highly regulated expression pattern, as was seen for the NLRR family. FLRT2 is expressed in a subset of the sclerotome, adjacent to the region that forms the syndetome, suggesting that interaction with FGF signalling may be a general property of FLRT proteins. All FLRTs can interact with FGFR1 and FLRTs can be induced by the activation of FGF signalling by FGF-2. FLRT proteins have a dual role, promoting FGF signalling and modulating homotypic cell adhesion. FLRT2 played critical roles in craniofacial development, and it was also present in the vomero-nasal organ, mandibular primodia, and the posterior aspects of the unfused and fused secondary palatal shelves.
Description	A DNA sequence encoding the human FLRT2 (O43155) extracellular domain (Met 1-Ser 539) was expressed, with a C-terminal His tag.
Source	HEK293
Predicted N Terminal	Cys 36
AA Sequence	Met 1-Ser 539
Molecular Weight	The secreted recombinant human FLRT2 comprises 515 a.a. and has a calculated molecular mass of 57.7 kDa. The apparent molecular mass of the recombinant protein is approximately 75 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Purity	>98% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Measured by the ability of the immobilized protein to support the adhesion of Neuro-2A mouse neuroblastoma cells.When cells are added to coated plates(5ug/mL, 100uL/well), approximately 50%-70% will adhere after 1 hour at 37°C.
Formulation	Lyophilized from sterile PBS, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.