Recombinant Human Fibroblast Growth Factor 10 Protein (FGF10), Active

Name: Recombinant Human Fibroblast Growth Factor 10 Protein (FGF10), Active
Brand: Beta LifeScience
SKU: BLC-05985P
Availability: InStock

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Product Overview

Description	Recombinant Human Fibroblast Growth Factor 10 Protein (FGF10), Active is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 97% as determined by SDS-PAGE and HPLC.
Endotoxin	Less than 1.0 EU/μg as determined by LAL method.
Activity	Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of >2.0x10 6 IU/mg.
Uniprotkb	O15520
Target Symbol	FGF10
Synonyms	BB213776 ; fd11d03; FGF 10; FGF-10; FGF10; FGF10_HUMAN; Fibroblast growth factor 10; Keratinocyte growth factor 2; KGF 2; Produced by fibroblasts of urinary bladder lamina propria; wu:fd11d03; zgc:109774
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	Tag-Free
Complete Sequence	LGQDMVSPEA TNSSSSSFSS PSSAGRHVRS YNHLQGDVRW RKLFSFTKYF LKIEKNGKVS GTKKENCPYS ILEITSVEIG VVAVKAINSN YYLAMNKKGK LYGSKEFNND CKLKERIEEN GYNTYASFNW QHNGRQMYVA LNGKGAPRRG QKTRRKNTSA HFLPMVVHS
Expression Range	40-208aa
Protein Length	Partial
Mol. Weight	19.1 kDa
Research Area	Cancer
Form	Lyophilized powder
Buffer	Lyophilized from a 0.2 µm filtered 2 × PBS, pH 7.4
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. May play a role in wound healing.
Subcellular Location	Secreted.
Protein Families	Heparin-binding growth factors family
Database References	HGNC: 3666 OMIM: 149730 KEGG: hsa:2255 STRING: 9606.ENSP00000264664 UniGene: PMID: 29053399 We have revealed significant association of FGFR2 and MAP3K1 polymorphisms with breast cancer. PMID: 29372690 data suggest that the strongest signal of association at 5p12 is mediated through coordinated activation of FGF10 and MRPS30, two candidate genes for breast cancer pathogenesis PMID: 27640304 Fgf10 signaling has an essential role in the formation of lipofibroblasts during late lung development PMID: 26511927 Expression of Fibroblast Growth Factor 10 is correlated with poor prognosis in gastric adenocarcinoma. PMID: 26268776 FGF10 has a role in protecting neuron against oxygen-glucose deprivation injury through inducing heme oxygenase-1 PMID: 25446127 Data identify autocrine activation of FGF signaling as an essential mechanism in promoting Pten-deficient skin tumors. PMID: 24582960 The therapeutic potential of the FGF10 treatment. PMID: 24865969 FGF10 plays an important role for tumor growth by both paracrine and autocrine manner. PMID: 24320134 The findings show that immunohistochemistry with FGF10, FGFR2b, or SHH could be useful in differentiating CCAM from type I PPB, when a child presents with a focal cystic lung lesion. PMID: 24004862 Paracrine FGF10 signaling stimulates the differentiation of human stem cell into urothelial cells. PMID: 23949743 High FGF10 expression is associated with ameloblastoma. PMID: 24002438 Three FGF10 single nucleotide polymorphisms in complete linkage disequilibrium--rs339501, rs12517396, and rs10462070--were associated with extreme myopia in the Japanese population. PMID: 24265547 FGF-10 expression during the development of the human hindgut and anorectum suggests that it may play a role in hindgut and anorectal morphogenesis. PMID: 23774963 The sclera of myopic eyes had higher FGF10 levels. The risk G allele of SNP rs339501 was associated with extreme myopia in human and caused a higher gene expression in the luciferase assay. PMID: 23599340 the posttranslational and transcriptional mechanisms underlying stimulation of P-glycoprotein function and expression by keratinocyte growth factor-2 (KGF2) that may contribute to the beneficial effects of KGF2 in intestinal inflammatory disorders PMID: 23328208 tooth agenesis had increased risk of a family history of cancer. tooth agenesis was associated with positive self-reported family history of cancer and variants in FGF10. PMID: 23169889 Identification of FGF-10 at both protein and mRNA levels in ovaries from fetuses, girls, and women suggests that FGF-10 contributes to preantral follicle development; FGF-10 is expressed in both granulosa cells and oocytes. PMID: 22877940 Our data suggest that common variants in FGF10 increase the risk for a wide range of non-syndromic limb deficiencies. PMID: 22965740 [review] FGF10 mediates biological responses by activating FGF receptor 2b (FGFR2b) in a paracrine manner. PMID: 21696361 The relationship between human ISL1 and FGF10 within the embryonic time window during which the linear heart tube remodels into four chambers, was examined. PMID: 22303449 Investigation of the pulmonary functions of COPD patients heterozygous for loss of function mutations in the FGF10 gene was performed. The patients show a significant decrease in lung function parameters when compared to control values. PMID: 21742743 Gremlin-mediated BMP inhibition results in activation of epithelial cells and transient fibrosis, but also induction of epithelium-protective FGF10 PMID: 20705941 FGF2 and FGF10 regulate migratory activity of ovine trophoblast cells through MAPK-dependent pathways. PMID: 21310815 FGF10 can promote the adipogenesis effect in situ. PMID: 19915940 There was no association among gene FGFR1 rs13317, p. E467K, p. M369I, p. S393S and gene FGF10 rs1448037 and nonsyndromic cleft lip with or without palate in Chinese population. PMID: 19727229 These results suggested that Thr-114 is a crucial functional residue for FGF10, and mutating Thr-114 to Ala or Arg would lead to great decrease in receptor-binding affinity and biological activity of FGF10. PMID: 20036575 FGF10 upregulates Na(+)-K(+)-exchanging ATPase via the MAPK pathway. PMID: 12804770 FGF-10 attenuates H2O2-induced alveolar epithelium DNA damage by mechanisms that involve activation of Grb2-SOS/Ras/RAF-1/ERK1/2 pathway and DNA repair. PMID: 14975937 Fgf10 mRNA is overexpressed in a subset of human breast carcinomas. PMID: 15208658 Data validate the symmetric two-end model of fibroblast growth factor (FGF) receptor (FGFR) dimerization and FGF binding and argue against the asymmetric model of FGFR dimerization. PMID: 15632068 We suggest that haploinsufficiency for FGF10 during a crucial stage of development results in ALSG. PMID: 15654336 Human embryonic pancreatic mesenchyme expresses FGF10, which might be used used to expand human embryonic pancreatic epithelial cells. PMID: 15690149 Human recombinant FGF10 maintains murine Notch activation and induces the expansion of murine pancreatic precursors while blocking their differentiation. PMID: 16323074 Localization of the FGF-10 receptor to the urothelial layer is clinically significant because intravesical administration of FGF-10 may provide a means to control the turnover of transitional epithelium in bladder disorders such as interstitial cystitis. PMID: 16597614 Activation of Toll-like receptor 2 (TLR2) or Toll-like receptor 4 (TLR4) inhibited FGF-10 expression, leading to abnormal saccular airway morphogenesis. PMID: 17071719 R80S and G138E mutations are associated with aplasia of the lacrimal and salivary glands. PMID: 17213838 a nuclear localization signal-like motif of FGF-10 is a partial determinant of its intracellular distribution and is necessary for its mitogenic activity PMID: 17471512 Trophoblast outgrowth and invasion (part of placental villi sprouting) at the fetal maternal interface is in part under delicate control of FGF 10 and Sprouty 2. PMID: 17496316 Results suggest that Lacrimo-auriculo-dento-digital syndrome is caused by reduced activity of the fibroblast growth factor 10 (FGF10)-FGF receptor 2 signaling pathway. PMID: 17682060 Expression in diverse niches of adult brain of Fgf10-lacZ reporter mice implicates Fgf10 in control of neurogenesis and/or conservation of neurogenic potential. PMID: 18329286 It cannot be ruled out that other genes involved in the signalling pathway of FGF10 may contribute to the formation of these congenital malformations. PMID: 18587586 Stromal FGF10 induces migration and invasion in pancreatic cancer cells through interaction with FGFR2, resulting in a poor prognosis. PMID: 18594526 family-based approach revealed an intronic variation of the FGF10 gene causing aplasia of lacrimal and salivary glands-syndrome PMID: 19102732 Disruption of localized pattern of mesenchymal fibroblast growth factor 10 expression results in impairment of lung branching morphogenesis, which progresses to emphysematous airspaces in adults. PMID: 19115389 Fgf10 is a strong causative candidate for defects observed in Apert syndrome since its genetic knockdown in a mouse model of Apert syndrome results in the rescue of the skeletal and visceral defects associated with this congenital disease. PMID: 18773495 An analysis of the distribution and fate of Fgf10-expressing cells in the adult mouse brain suggests that Fgf10 may have critical regulatory roles in stem cell function and generation of new neurons in diverse areas of the adult brain. PMID: 18773495 TGFbeta1 caused a rapid and transient decrease in Fgf10 mRNA levels in primary prostatic cells. Deletion analysis of the Fgf10 promoter identified a region that mediated a proportion of promoter activity as well as promoter down-regulation by TGFbeta1. PMID: 14726452

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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