Recombinant Human CD16b Protein (C-Fc-6His)

Name: Recombinant Human CD16b Protein (C-Fc-6His)
Brand: Beta LifeScience
SKU: BL-0731NP
Availability: InStock

BL-0731NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: Fc receptors, Featured fc receptors molecules, High-quality recombinant proteins

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Product Overview

Description	Recombinant Human Low Affinity Immunoglobulin Gamma Fc Region Receptor III-B is produced by our Mammalian expression system and the target gene encoding Gly17-Gln208 is expressed with a human IgG1 Fc tag at the C-terminus.
Accession	O75015
Synonym	Low affinity immunoglobulin gamma Fc region receptor III-B; Fc-gamma RIII-beta; FcR-10; IgG Fc receptor III-1; FCG3; FCGR3; CD16b; FCGR3B
Gene Background	Low affinity immunoglobulin gamma Fc region receptor III-B, also known as Fc-gamma RIII-beta, FcR-10, IgG Fc receptor III-1, FCG3, FCGR3, CD16b and FCGR3B. FCGR3B is a GPI-anchor membrane protein and contains two Ig-like C2 type domains. FCGR3B can be expressed in orphonuclear leukocytes and stimulated eosinophils. FCGR3B can interact with INPP5D/SHIP1. FCGR3B localizes in the FCGR gene cluster is a CN polymorphic gene involved in the recruitment of polymorphonuclear neutrophils to sites of inflammation and their activation. FCGR3B may serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.
Molecular Mass	48.8 KDa
Apmol Mass	70 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.
Subcellular Location	Cell membrane; Lipid-anchor, GPI-anchor. Secreted. Note=Secreted after cleavage.
Database References	HGNC: 3620 OMIM: 610665 KEGG: hsa:2215 STRING: 9606.ENSP00000294800 UniGene: PMID: 29137913 FCGR3B deficiency due to copy number variations may predispose to eosinophilic granulomatosis with polyangiitis. PMID: 26684293 The FCGR3B NA1/NA1 genotype is associated with hypersensitivity reactions to adalimumab in Japanese patients with Rheumatoid Arthritis. PMID: 27830964 Data suggest association of FCGR3B deletion with increased risk of rheumatoid arthritis (RA) in a large cohort. PMID: 27995740 Association between Fc gamma Receptor IIIB genetic polymorphisms and susceptibility to severe malaria anemia in children in western Kenya. PMID: 28427365 CD16 and CD35 are important for neutrophil internalization of M. tuberculosis, whereas HIV-1 infection adversely affects opsonophagocytosis. PMID: 27258232 There was no aberration in FCGR3B allele/genotype frequencies in sarcoidosis patients compared to controls. PMID: 26801149 Results showed that susceptibility to systemic lupus erythematosus was associated with the FCGR3B01 allele, as well as with the FCGR3B01/01 and FCGR3B01/02 genotypes. PMID: 26946294 FcgammaRIIIB allelic distribution was similar among pediatric Guillain-Barre syndrome patients and controls. PMID: 27064330 data show that cross-linking FcgammaRIIIb is responsible for NET formation by the human neutrophil PMID: 27034964 the results of this meta-analysis indicated that low FCGR3B CN increased susceptibility to autoimmune diseases--{REVIEW} PMID: 26407570 FCGR3B copy number related to systemic lupus and lupus nephritis susceptibility. PMID: 24673810 No relationship was found between the FCGR3B polymorphism and rheumatoid arthritis susceptibility. PMID: 26314337 This study compared the FCGR3B gene frequencies among 230 unrelated healthy Eastern Thai blood donors in Rayong hospital with the previously published studies. PMID: 25626603 FcgammaRIIIb polymorphism is not associated with susceptibility to systemic lupus erythematosus in Brazilian patients PMID: 24896836 in the Indian population, FCGR3B gene copy number varied significantly when compared to other populations of the world. PMID: 25428402 A high copy number of FCGR3B is associated with psoriasis vulgaris in Han Chinese. PMID: 25012234 This suggests that FcgammaRIIIb signals in association with macrophage-1 Ag. PMID: 25024378 The frequencies of FCGR3B1, FCGR3B2, and FCGR3B3 alleles in central Thai blood donors were 0.548, 0.452, and 0.004. PMID: 24205492 Copy number of polymorphisms in FCGR3B gene is associated with ulcerative colitis. PMID: 23917248 The absolute risk of malaria decreased more rapidly with increasing antibody levels for Fc gamma receptor 3B 233AA/AC individuals compared with 233CC children. PMID: 23935200 The FCGR3B NA1/NA2 polymorphism may be associated with susceptibility to aggressive periodontitis. PMID: 23649770 study concludes gene copy number of FcgammaR2C and FcgammaR3B influences IVIG treatment response and predisposes individuals to Kawasaki disease, providing potential insights into understanding the mechanism of the FcgammaR gene family in the IVIG pathway PMID: 23778324 Allele frequencies were determined in the blood donor population as follows: 0.318 for HNA-1a, 0.668 for HNA-1b, 0.014 for HNA-1c, 0.768 for HNA-3a, 0.232 for HNA-3b, 0.882 for HNA-4a, 0.118 for HNA-4b, 0.736 for HNA-5a and 0.264 for HNA-5b. PMID: 23398146 Higher CD16+ cytotoxic NK+T lymphocyte levels predict superior survival outcomes in patients with metastatic carcinoma. PMID: 23281059 Low copy number of the Fc-gamma receptor 3B gene FCGR3B is a risk factor for primary Sjogren's syndrome. PMID: 22942264 Studies indicate beta-defensins (DEFB4, DEFB103, DEFB104), chemokine ligand 3 like 1 (CCL3L1), Fc gamma receptor 3B (FCGR3B), and complement component C4 (C4) for copy number variation in disease association. PMID: 22837109 The FcgammaRIIIB-SH allotype (CTGAAA) containing the 233A-allele (in bold) was associated with protection from malaria. The FcgammaRIIIB-NA203 allotype (CTGCGA), a variant of the classical FcgammaRIIIB-NA2 (CTGCAA) was associated with susceptibility to malaria. PMID: 23049979 suggest that impaired immune complex clearance arising from FCGR3B deficiency contributes to the pathology of systemic sclerosis, and FCGR3B copy number variation is a common risk factor for systemic autoimmunity PMID: 22551723 CD14++CD16+ monocytes independently predicted cardiovascular events in subjects referred for elective coronary angiography. PMID: 22999728 Study indicates that a low copy number of the FCGR3B gene is associated with susceptibility to rheumatoid arthritis (RA). PMID: 22309893 The FcgammaRIIIB-NA1 variant, which confers higher affinity for IgG than the NA2 variant, is a determining factor for treatment response. PMID: 22565545 FCGR3B1, FCGR3B2 and FCGR3B3 allele frequencies were respectively 0.347, 0.573 and 0.080 among Tunisians of sub-Saharan origin, 0.379, 0.591 and 0.030 among Tunisian blood donors and 0.318, 0.546 and 0.136 among the group from sub-Saharan Africa. PMID: 22512913 Both ADAM10 and ADAM17 could shed CD16b, but they possess differed preferences. ADAM10 is the main sheddase under stimulation of ionomycin, while ADAM17 is the main sheddase under stimulation of PMA. PMID: 22770404 NA2/NA2 and/or NA2 allele may be risk factors for generalized aggressive periodontitis in the population of South India PMID: 22167032 In this Brazilian FCGR3B03(+) group we found that the inheritance of FCGR3B03 took place by a linkage to FCGR3B02 or to FCGR3B01. PMID: 21895673 FCGR3B DNA copy number variation is associated with systemic lupus erythematosus risk in afro-caribbeans. PMID: 21296850 Monocyte subsets have specialized roles during inflammation: CD16-positive monocytes might undergo an immediate innate immune response, whereas CD16-negative monocytes could have a major role as antigen presenting cells. PMID: 21937707 Our results suggest that FcgammaRIIIb might not be a susceptibility gene for SLE and lupus nephritis. PMID: 20300756 copy number associated with susceptibility to idiopathic pulmonary fibrosis PMID: 21109729 IL-21 stimulates the production of IL-10 by CD4(+) T-cells. Secreted Il-10 maintains the expression of CD16 on monocytes. PMID: 21227406 the neutrophil responses to stimuli that engage FcGR3B and FcGR2A, namely the phagocytosis of human IgG-opsonized zymosan and the responses to heat-aggregated IgGs PMID: 21123174 FcgammaRIIIb NA1/2 polymorphisms are associated with idiopathic pulmonary fibrosis disease susceptibility but not with disease progression. PMID: 20924590 study highlights the complexity and poor characterization of the FCGR3B gene sequence, indicating that the design and interpretation of genotyping assays based on specific probe sequences must be performed with caution PMID: 20957197 The FCGR3B NA1/NA1 genotype was found in 75% of the patients with infusion reactions and in only 37% of those without (p=0.01), whereas the FCGR3A 176F/V genotype was equally distributed in the patients with or without infusion reactions. PMID: 20980704 FcgammRIIA and FcgammaRIIIB are required for neutrophil mediated dermal epidermal separation in bullous pemphigoid PMID: 20720565 results, obtained by fitting logistic regression models, confirm the association of low copy number of FCGR3B with systemic lupus erythematosus PMID: 20442749 Results of the present study suggest that subjects carrying at least one copy of the FcgammaRIIIb-NA2 allele might be associated with susceptibility to aggressive periodontitis. PMID: 20041976 The primary involvement of Fc gamma R IIIB NA2 allele is more likely involved with disease susceptibility of systemic lupus erythematosus. PMID: 20536598 Significant evidence for an association between copy number and rheumatoid arthritis was observed in the Dutch cohort but not in the two smaller cohorts (New Zealand and United Kingdom populations, respectively). PMID: 20472591

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.