Recombinant Human FcγR2IA Protein (His Tag)

Beta LifeScience SKU/CAT #: BL-0326NP
Recombinant Human FcγR2IA Protein (His Tag)

Recombinant Human FcγR2IA Protein (His Tag)

Beta LifeScience SKU/CAT #: BL-0326NP
Catalog No.: BL-0326NP

Product Overview

Tag His
Host Species Human
Accession P08637
Synonym FcγRIIIA Low Affinity Immunoglobulin Gamma Fc Region Receptor III-A; CD16a Antigen; Fc-Gamma RIII-Alpha; Fc-Gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcR-10; IgG Fc Receptor III-2; CD16a; FCGR3A; CD16A; FCG3; FCGR3; IGFR3
Background Receptors for the Fc region of immunoglobin G (FcγR) are divided into three classes and FcγRIII is a multifunctional, low/intermediate affinity receptor. In humans, FcγRIII is expressed as two distinct forms (FcγRIIIA and FcγRIIIB) that are encoded by two different but highly homologous genes in a cell type-specific manner. FcγRIIIB is a low-affinity, GPI-linked receptor expressed by neutrophils and eosinophils, whereas FcγRIIIA is an intermediate affinity polypeptide-anchored transmembrane glycoprotein expressed by a subset of T lymphocytes, natural killer (NK) cells, monocytes, and macrophages. The FcγRIIIA receptor is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), mast cell degranulation, and clearance of immune complexes. FcγRIIIA has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain and delivers an activation signal in the immune responses. Aberrant expression or mutations in this gene is implicated in susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia. In humans, it is a 50 -70 kD type I transmembrane activating receptor. The FcγRIIIA cDNA encodes 254 amino acid including a 16aa signal sequence, 191 amino acid ECD with two C2-type Ig-like domains, five potential N-glycosylation sites, a 22 amino acid transmembrane sequence and a 25 amino acid cytoplasmic domain.
Description Recombinant Human Low Affinity Immunoglobulin Gamma Fc Region Receptor III-A is expressed by our Mammalian expression system and the target gene encoding Gly17-Gln208 is expressed with a His tag at the C-terminus.
Source Human Cells
AA Sequence Gly17-Gln208
Purity >95% as determined by reducing SDS-PAGE.
Endotoxin <0.1 ng/µg (1 EU/µg) as determined by LAL test.
Bioactivity Measured by its ability to bind Human IGHG1 in functional ELISA. The ED50 for this effect is less than 20 ug/ml.
Formulation Recombinant Human FcγRIIIA Protein was lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Reconstitution Dissolve the lyophilized protein in distilled water.
Usage For Research Use Only
Storage Human FcγRIIIA protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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