Recombinant Human Fas Ligand Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-1987
Recombinant Human Fas Ligand Protein (His Tag)

Recombinant Human Fas Ligand Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-1987

Product Overview

Tag His
Host Species Human
Accession NP_000630.1
Synonym ALPS1B, APT1LG1, APTL, CD178, CD95-L, CD95L, FASL, FASLG, TNFSF6, TNLG1A
Background Fas Ligand, also known as FASLG and CD95L, is the ligand for FAS. It is a transmembrane protein which binds to TNFRSF6/FAS. Interaction of FAS with fas Ligand is critical in triggering apoptosis of some types of cells such as lymphocytes. Fas Ligand may be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both.
Description A DNA sequence encoding human FASLG (NP_000630.1) (Pro134-Leu281) was expressed with a His tag at the N-terminus.
Source Yeast
Predicted N Terminal His
AA Sequence Pro134-Leu281
Molecular Weight The recombinant human human FASLG consists of 168 a.a. and predicts a molecular mass of 19.3 KDa. It migrates as an approximately 26 KDa band in SDS-PAGE under reducing conditions.
Purity >90% as determined by SDS-PAGE
Endotoxin Please contact us for more information.
Bioactivity Measured by its binding ability in a functional ELISA.1. Immobilized human His-FASLG at 10 ug/ml (100 ul/well) can bind Cynomolgus FAS-Fc. The EC50 of Cynomolgus FAS-Fc is 0.06-0.14 ug/ml.2. Immobilized human His-FASLG at 10 ug/ml (100 ul/well) can bind rat FAS-Fc. The EC50 of rat FAS-Fc is 0.22-0.52 ug/ml.
Formulation Lyophilized from sterile PBS, pH 7.4..
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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