Recombinant Human Fas Ligand Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1987
Recombinant Human Fas Ligand Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1987
Collections: Other recombinant proteins, Recombinant proteins
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | NP_000630.1 |
| Synonym | ALPS1B, APT1LG1, APTL, CD178, CD95-L, CD95L, FASL, FASLG, TNFSF6, TNLG1A |
| Background | Fas Ligand, also known as FASLG and CD95L, is the ligand for FAS. It is a transmembrane protein which binds to TNFRSF6/FAS. Interaction of FAS with fas Ligand is critical in triggering apoptosis of some types of cells such as lymphocytes. Fas Ligand may be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. |
| Description | A DNA sequence encoding human FASLG (NP_000630.1) (Pro134-Leu281) was expressed with a His tag at the N-terminus. |
| Source | Yeast |
| Predicted N Terminal | His |
| AA Sequence | Pro134-Leu281 |
| Molecular Weight | The recombinant human human FASLG consists of 168 a.a. and predicts a molecular mass of 19.3 KDa. It migrates as an approximately 26 KDa band in SDS-PAGE under reducing conditions. |
| Purity | >90% as determined by SDS-PAGE |
| Endotoxin | Please contact us for more information. |
| Bioactivity | Measured by its binding ability in a functional ELISA.1. Immobilized human His-FASLG at 10 ug/ml (100 ul/well) can bind Cynomolgus FAS-Fc. The EC50 of Cynomolgus FAS-Fc is 0.06-0.14 ug/ml.2. Immobilized human His-FASLG at 10 ug/ml (100 ul/well) can bind rat FAS-Fc. The EC50 of rat FAS-Fc is 0.22-0.52 ug/ml. |
| Formulation | Lyophilized from sterile PBS, pH 7.4.. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
