Recombinant Human ESAM Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-1927

Recombinant Human ESAM Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-1927
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Product Overview

Tag His
Host Species Human
Accession NP_620411.2
Synonym W117m
Background Endothelial cell-selective adhesion molecule (ESAM) is a member of JAM family of immunoglobulin superfamily and consists of one V-type and one C2-type immunoglobulin domain, as well as a hydrophobic signal sequence, a single transmembrane region, and a cytoplasmic domain. It is specifically expressed at endothelial tight junctions and on activated platelets. ESAM at endothelial tight junctions participates in the migration of neutrophils through the vessel wall, possibly by influencing endothelial cell contacts. The adaptor protein membrane-associated guanylate kinase MAGI-1 has been identified as an intracellular binding partner of ESAM. Previous studies have indicated that ESAM regulates angiogenesis in the primary tumor growth and endothelial permeability. It suggest that ESAM has a redundant functional role in physiological angiogenesis but serves a unique and essential role in pathological angiogenic processes such as tumor growth.
Description A DNA sequence encoding the extracellular domain (Met 1-Ala 248) of human ESAM (NP_620411.2) precursor was expressed,with a His tag at the C-terminus.
Source HEK293
Predicted N Terminal Gln 30
AA Sequence Met 1-Ala 248
Molecular Weight The recombinant human ESAM consists of 230 a.a. and predicts a molecular mass of 25.4 kDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of rh ESAM is approximately 35-40 kDa due to glycosylation.
Purity >97% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Please contact us for detailed information
Formulation Lyophilized from sterile PBS, pH 7.4.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function Can mediate aggregation most likely through a homophilic molecular interaction.
Subcellular Location Cell junction, adherens junction. Cell junction, tight junction. Cell membrane; Single-pass type I membrane protein.
Database References
Tissue Specificity Highly expressed in endothelial cells.

Gene Functions References

  1. sESAM levels associate with myocardial infarction, heart failure, and death after adjustment for demographic and clinical risk factors, but not after adjustment for kidney function. PMID: 26523992
  2. ESAM is a novel human hematopoietic stem cell marker associated with a subset of human leukemias PMID: 26774386
  3. Serum ESAM is higher in type 2 diabetes mellitus than in controls and parallels oxidative stress. PMID: 24456423
  4. sESAM is associated with albuminuria and reduced kidney function in patients with coronary artery disease in both cross-sectional and longitudinal analyses. PMID: 24177327
  5. ESAM regulates tumor metastasis through endothelial cell migration and tube formation in metastatic nodules. PMID: 20153339
  6. sESAM is a predictive biomarker and potential mediator of atherosclerosis. PMID: 19759376

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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