Recombinant Human ESAM Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1927
Recombinant Human ESAM Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1927
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Human |
Accession | NP_620411.2 |
Synonym | W117m |
Background | Endothelial cell-selective adhesion molecule (ESAM) is a member of JAM family of immunoglobulin superfamily and consists of one V-type and one C2-type immunoglobulin domain, as well as a hydrophobic signal sequence, a single transmembrane region, and a cytoplasmic domain. It is specifically expressed at endothelial tight junctions and on activated platelets. ESAM at endothelial tight junctions participates in the migration of neutrophils through the vessel wall, possibly by influencing endothelial cell contacts. The adaptor protein membrane-associated guanylate kinase MAGI-1 has been identified as an intracellular binding partner of ESAM. Previous studies have indicated that ESAM regulates angiogenesis in the primary tumor growth and endothelial permeability. It suggest that ESAM has a redundant functional role in physiological angiogenesis but serves a unique and essential role in pathological angiogenic processes such as tumor growth. |
Description | A DNA sequence encoding the extracellular domain (Met 1-Ala 248) of human ESAM (NP_620411.2) precursor was expressed,with a His tag at the C-terminus. |
Source | HEK293 |
Predicted N Terminal | Gln 30 |
AA Sequence | Met 1-Ala 248 |
Molecular Weight | The recombinant human ESAM consists of 230 a.a. and predicts a molecular mass of 25.4 kDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of rh ESAM is approximately 35-40 kDa due to glycosylation. |
Purity | >97% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Please contact us for detailed information |
Formulation | Lyophilized from sterile PBS, pH 7.4. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Target Details
Target Function | Can mediate aggregation most likely through a homophilic molecular interaction. |
Subcellular Location | Cell junction, adherens junction. Cell junction, tight junction. Cell membrane; Single-pass type I membrane protein. |
Database References | |
Tissue Specificity | Highly expressed in endothelial cells. |
Gene Functions References
- sESAM levels associate with myocardial infarction, heart failure, and death after adjustment for demographic and clinical risk factors, but not after adjustment for kidney function. PMID: 26523992
- ESAM is a novel human hematopoietic stem cell marker associated with a subset of human leukemias PMID: 26774386
- Serum ESAM is higher in type 2 diabetes mellitus than in controls and parallels oxidative stress. PMID: 24456423
- sESAM is associated with albuminuria and reduced kidney function in patients with coronary artery disease in both cross-sectional and longitudinal analyses. PMID: 24177327
- ESAM regulates tumor metastasis through endothelial cell migration and tube formation in metastatic nodules. PMID: 20153339
- sESAM is a predictive biomarker and potential mediator of atherosclerosis. PMID: 19759376