Recombinant Human EPO Protein, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0001UL
Recombinant Human EPO protein (2 μg/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie® Blue showing a single band from 30-45 kDa and purity greater than 98%.
Recombinant Human EPO protein (2 μg/lane) on SDS-PAGE under reducing (R) conditions. The gel was stained using Coomassie® Blue showing a single band from 30-45 kDa and purity greater than 98%.

Recombinant Human EPO Protein, Active, Low Endotoxin

Beta LifeScience SKU/CAT #: BEP-0001UL
/
Size

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Product Overview

Product Name Recombinant Human EPO Protein
Target Symbol EPO
Alternative Names Erythropoietin, Epoetin, EP, ECYT5, MVCD2, DBAL
Accession Number P01588
Expression System HEK293 Cell
Molecular Weight 21 kDa (monomer, predicted), 30-45 kDa (observed)
Sequence Ala28-Arg193
Purity >98% by SDS-PAGE and quantitative densitometry by Coomassie® Blue staining
Activity Determined by a cell proliferation assay using TF-1 cells. The ED50 for this effect is typically <0.5 ng/mL.
Formulation Lyophilized from sterile PBS with Trehalose, pH 7.4
Endotoxin Level <0.001 EU per 1 µg of the protein as determined by the LAL method
Shipping Shipped at ambient temperature.
Stability & Storage 12 months from date of receipt at -20°C to -70°C, lyophilized powder. 3 months at -20°C to -70°C under sterile conditions after reconstitution. Avoid repeated freeze-thaw cycles.
Reconstitution Reconstitute at 100 μg/mL in PBS. Optionally, use PBS with 0.1% BSA.
Target Function EPO is the primary regulator of red blood cell production. It acts as a growth factor that promotes erythroid differentiation, stimulates red blood cell maturation, and initiates hemoglobin synthesis. It acts by binding to the Erythropoietin Receptor (EPOR) on target cells, activating signalling pathways (like JAK2/STAT5) that prevent apoptosis (cell death) of erythroid progenitors.
Tissue Specificity The kidneys—specifically renal interstitial fibroblasts—are the primary source of this protein, while the liver serves as a secondary source, especially during fetal development. Despite the liver showing the highest mRNA expression, the functional protein is released into the bloodstream to act upon the bone marrow.
Cellular Localization Secreted protein
Involvement In Disease Low levels of erythropoietin (EPO) lead to anemia, especially in chronic kidney disease, while excessive production causes polycythemia by creating too many red blood cells. Abnormal EPO signaling is also linked to erythroleukemia, familial erythrocytosis, and potential tumor growth in glioma or cervical cancer. To treat anemia related to kidney disease, chemotherapy, or HIV, doctors use recombinant human EPO versions like epoetin alfa, beta, delta, and omega.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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