Recombinant Human Epithelial Discoidin Domain-Containing Receptor 1 (DDR1) Protein (His), Active

Name: Recombinant Human Epithelial Discoidin Domain-Containing Receptor 1 (DDR1) Protein (His), Active
Brand: Beta LifeScience
SKU: BLC-05744P
Availability: InStock

Greater than 95% as determined by SDS-PAGE.

Activity Measured by its binding ability in a functional ELISA. Immobilized Human DDR1 at 2 μg/ml can bind Anti-DDR1 recombinant antibody , the EC 50 is 2.824-3.810 ng/mL. Biological Activity Assay

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Product Overview

Description	Recombinant Human Epithelial Discoidin Domain-Containing Receptor 1 (DDR1) Protein (His), Active is produced by our Mammalian cell expression system. This is a protein fragment.
Purity	Greater than 95% as determined by SDS-PAGE.
Endotoxin	Less than 1.0 EU/ug as determined by LAL method.
Activity	Measured by its binding ability in a functional ELISA. Immobilized Human DDR1 at 2 μg/mL can bind Anti-DDR1 recombinant antibody , the EC 50 is 2.824-3.810 ng/mL.
Uniprotkb	Q08345
Target Symbol	DDR1
Synonyms	(Epithelial discoidin domain receptor 1)(CD167 antigen-like family member A)(Cell adhesion kinase)(Discoidin receptor tyrosine kinase)(HGK2)(Mammary carcinoma kinase 10)(MCK-10)(Protein-tyrosine kinase 3A)(Protein-tyrosine kinase RTK-6)(TRK E)(Tyrosine kinase DDR)(Tyrosine-protein kinase CAK)(CD antigen CD167a)
Species	Homo sapiens (Human)
Expression System	Mammalian cell
Tag	C-10His
Target Protein Sequence	DMKGHFDPAKCRYALGMQDRTIPDSDISASSSWSDSTAARHSRLESSDGDGAWCPAGSVFPKEEEYLQVDLQRLHLVALVGTQGRHAGGLGKEFSRSYRLRYSRDGRRWMGWKDRWGQEVISGNEDPEGVVLKDLGPPMVARLVRFYPRADRVMSVCLRVELYGCLWRDGLLSYTAPVGQTMYLSEAVYLNDSTYDGHTVGGLQYGGLGQLADGVVGLDDFRKSQELRVWPGYDYVGWSNHSFSSGYVEMEFEFDRLRAFQAMQVHCNNMHTLGARLPGGVECRFRRGPAMAWEGEPMRHNLGGNLGDPRARAVSVPLGGRVARFLQCRFLFAGPWLLFSEISFISDVVNNSSPALGGTFPPAPWWPPGPPPTNFSSLELEPRGQQPVAKAEGSPTA
Expression Range	21-417aa
Protein Length	Partial
Mol. Weight	46.8 kDa
Form	Lyophilized powder
Buffer	Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing. Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing. Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Also plays a role in tumor cell invasion. Phosphorylates PTPN11.
Subcellular Location	[Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Cell membrane; Single-pass type I membrane protein.; [Isoform 3]: Secreted.; [Isoform 4]: Cell membrane; Single-pass type I membrane protein.
Protein Families	Protein kinase superfamily, Tyr protein kinase family, Insulin receptor subfamily
Database References	HGNC: 2730 OMIM: 600408 KEGG: hsa:780 STRING: 9606.ENSP00000365759 UniGene: PMID: 29298894 our study provides a novel regulatory pathway involving TM4SF1, DDR1, MMP2 and MMP9, which promotes the formation and function of invadopodia to support cell migration and invasion in pancreatic cancer. PMID: 28368050 The three well-conserved seed matched sites for miR-199a/b-5p in the discoidin domain receptor 1 (DDR1) 3'-UTR were targeted, and miRNA binding to at least two sites was required for DDR1 inhibition. PMID: 29429150 E2F1 knockdown decreased the expression of discoidin domain receptor 1 (DDR1) which plays a crucial role in many fundamental processes such as cell differentiation, adhesion, migration and invasion. PMID: 29039472 Furthermore, the inhibition of DDR1 with 7rh showed striking efficacy in combination with chemotherapy in orthotopic xenografts and autochthonous pancreatic tumors where it significantly reduced DDR1 activation and downstream signaling, reduced primary tumor burden, and improved chemoresponse. PMID: 28864681 These findings demonstrate a critical role of miR-199a-3p/DDR1 pathway in ovarian cancer development. PMID: 28743276 findings demonstrate that, in human breast cancer cells, DDR1 regulates IR expression and ligand dependent biological actions. This novel functional crosstalk is likely clinically relevant PMID: 28591735 These results support an activation mechanism of DDR1 whereby collagen induces lateral association of DDR1 dimers and phosphorylation between dimers. PMID: 28590245 Data suggest that IGF-I/IGF-IR system triggers stimulatory actions through both GPER and DDR1 in aggressive tumors as mesothelioma and lung tumors. PMID: 27384677 we further analyzed the CpG methylation levels at the DDR1 promoter in EOC cells and found that the CpG methylation levels of DDR1 promoter correlated negatively with the expression of DDR1 along the EMT spectrum. Therefore, EMT stratification could be a potential biomarker to predict patient response to DDR1-targeting drugs. PMID: 28887161 This study suggested that DDR1 and DDR2 knockdown alters brain immunity and significantly reduces the level of triggering receptor expressed on myeloid cells (TREM)-2 and microglia. PMID: 28863860 Isoform b of DDR1 is responsible for collagen I-induced up-regulation of N-cadherin and tyrosine 513 of DDR1b is necessary. PMID: 27605668 Reduced DDR1 expression may be implicated in impaired melanocyte adhesion process involved in vitiligo pathogenesis PMID: 26091274 data demonstrate that TGF-beta1 favors linear invadosome formation through the expressions of both the inducers, such as collagen and LOXL2, and the components such as DDR1 and MT1-MMP of linear invadosomes in cancer cells. Meanwhile, our data uncover a new TGF-beta1-dependent regulation of DDR1 expression. PMID: 27720259 DDR1 overexpression promoted GC cell proliferation (p < 0.05), migration (p < 0.01), and invasion (p < 0.01), and accelerated the growth (p < 0.05) as well as the microvessel formation (p < 0.01) of transplantation tumor in nude mice. PMID: 27179963 Our results suggest that DDR1 is both a prognostic marker for renal clear cell carcinoma and a potential functional target for therapy PMID: 27020590 Study concludes that non-canonical DDR1 signaling enables breast cancer cells to exploit the ubiquitous interstitial matrix component collagen I to undergo metastatic reactivation in multiple target organs. PMID: 27368100 Upregulation of DDR1 collagen receptor is associated with breast cancer. PMID: 26655502 Knockdown DDR1 reversed the effects of Galpha13 knockdown on cell-cell adhesion and proteolytic invasion in three-dimensional collagen. PMID: 26589794 Data suggest SCl2 (Streptococcus pyogenes) binds to DDR (DDR1, DDR2) ectodomain w/o stimulating receptor signaling; here, protein engineering was used to construct SCl-like proteins that inhibit collagen-DDR interactions and macrophage migration. PMID: 26702058 DDR1 is a key modulator of RIT activity. PMID: 26719540 DDR1 expression is a prognostic indicator in pancreatic ductal carcinoma. PMID: 26297342 alpha5(IV), but not alpha1(IV), promotes lung cancer cell proliferation and tumor angiogenesis through non-integrin collagen receptor DDR1-mediated ERK activation. PMID: 25992553 Hypoxia can increase DDR1 expression in pituitary adenoma cells, leading to improved MMP-2 and MMP-9 secretion, and promoting pituitary adenoma cell proliferation and invasion. PMID: 26286316 suggest that DDR1 suppression may enhance adipose-derived stem cell chondrogenesis by enhancing the expression of chondrogenic genes and cartilaginous matrix deposition. PMID: 25673773 MT1-MMP-discoidin domain receptor 1 axis regulates collagen-induced apoptosis in breast cancer cells PMID: 25774665 Defective Ca(2+) binding in a conserved binding site causes incomplete N-glycan processing and endoplasmic reticulum trapping of DDR1 and DDR2 receptors. PMID: 25470979 A DDR1(Low)/DDR2(High) protein profile is associated with TNBC and may identify invasive carcinomas with worse prognosis. PMID: 25667101 We uncovered ten new mutations in TNK2 and DDR1 within serous and endometrioid ECs, thus providing novel insights into the mutation spectrum of each gene in EC. PMID: 25427824 Silencing of DDR1 inhibited tumor cell growth and motility, and induced TGFBI expression, both in vitro and in vivo. PMID: 25369402 found an inverse correlation between ZEB1 and DDR1 expression in various cancer cell lines and in human breast carcinoma tissues PMID: 25155634 DDR1 depletion blocked cell invasion in a collagen gel PMID: 25422375 The expression of the DDR1 protein significantly correlated with poor disease-free survival in patients with serous ovarian cancer. PMID: 21541037 High DDR1 protein expression is associated with recurrence in ameloblastomas. PMID: 24723326 In this review we highlight the mechanisms whereby DDRs(DDR1 and DDR2) regulate two important processes, namely inflammation and tissue fibrosis. PMID: 24361528 Report aberrant methylation of DDR1 in men with nonobstructive azoospermia. PMID: 25064398 Crystal structures of DDR1 reveal a DFG-out conformation (DFG, Asp-Phe-Gly) of the kinase domain that is stabilized by a salt bridge between the activation loop and alphaD helix. Differences to Abelson kinase are observed in the DDR1 P-loop's beta-hairpin. PMID: 24768818 These findings indicate that the extracellular juxtamembrane region of DDR1 is exceptionally flexible and does not constrain the basal or ligand-activated state of the receptor. PMID: 24671415 N-glycosylation at the highly conserved (211)NDS motif evolved to act as a negative repressor of DDR1 phosphorylation in the absence of ligand PMID: 24509848 regulation of DDRs by glucose PMID: 24018687 Upregulation of DDR1 induced by collagen I may contribute to the development and progression of NSCLC. PMID: 23761027 The DDR1 extracellular domain plays a crucial role in receptor oligomerization which mediates high-affinity interactions with its ligand. PMID: 23810922 We show that expression of the latent LMP1 in primary human germinal center B cells, the presumed progenitors of HRS cells, upregulates discoidin domain receptor 1 (DDR1), a receptor tyrosine kinase activated by collagen. PMID: 24136166 these data suggest that NEP can augment taxane-induced apoptosis through inhibition of Akt/Bad signaling PMID: 22895534 the expression of the novel collagen receptor discoidin domain receptor 1 (DDR1) by human MKs at both mRNA and protein levels and provide evidence of DDR1 involvement in the regulation of MK motility on type I collagen PMID: 23530036 DDR1 expression was not predictive for patient survival in human breast cancer PMID: 23307244 DD1 mRNA and protein levels were higher in patients with recurrent Hepatocellular carcinoma , suggesting this gene maybe involved in tumor invasion and metastasis. PMID: 22752569 a role for the collagenase of membrane-type MMPs in regulation of DDR1 cleavage and activation at the cell-matrix interface. PMID: 23519472 Discoidin domain receptors promote alpha1beta1- and alpha2beta1-integrin mediated cell adhesion to collagen by enhancing integrin activation. PMID: 23284937 Discoidin domain receptors are unique receptor tyrosine kinases in collagen-mediated signaling [review] PMID: 23335507

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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