Recombinant Human ENPP7 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1764
Recombinant Human ENPP7 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1764
Catalog No.: BLPSN-1764
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | NP_848638.2 |
| Synonym | ALK-SMase, E-NPP7, NPP-7, NPP7 |
| Background | Ectonucleotide pyrophosphatase / phosphodiesterase family member 7, also known as Alkaline sphingomyelin phosphodiesterase, Intestinal alkaline sphingomyelinase, Alk-Smase, ENPP7 and NPP-7, is a single-pass type I membrane protein which belongs to thenucleotide pyrophosphatase / phosphodiesterase family. ENPP7 / NPP-7 is expressed in the intestines and human bile. ENPP7 / NPP-7 is localized at the surface of the microvillar membrane in small intestine enterocytes, as well as in endosome-like structures and in Golgi complex. The main function of ENPP7 / NPP-7 is to convert the dietary sphingomyelin into ceramide, the sphingolipid messengers via hydrolyzation. ENPP7 / NPP-7 is also reported to exert a phospholipase C activity toward palmitoyl lyso-phosphocholine. The activity of this enzyme is inhibited in a dose dependent manner by ATP, imidazole, orthovanadate and zinc ion. Further, It has been shown in studies that decreased levels of ENPP7 / NPP-7 may be associated with human colon cancer. |
| Description | A DNA sequence encoding the human ENPP7 (NP_848638.2) (Met 1-Ser 439) was fused with a C-terminal His tag. |
| Source | HEK293 |
| Predicted N Terminal | Ala 22 |
| AA Sequence | Met 1-Ser 439 |
| Molecular Weight | The secreted recombinant human ENPP7 comprises 429 a.a. with a predicted molecular mass of 49 kDa. As a result of glycosylation, rh ENPP7 migrates as an approximately 55-60 kDa band in SDS-PAGE under reducing conditions. |
| Purity | >95% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
