Recombinant Human Endothelin-3 (EDN3) Protein (His-Myc)

Name: Recombinant Human Endothelin-3 (EDN3) Protein (His-Myc)
Brand: Beta LifeScience
SKU: BLC-06624P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Collections: High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Endothelin-3 (EDN3) Protein (His-Myc) is produced by our Yeast expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	P14138
Target Symbol	EDN3
Synonyms	(ET-3)(Preproendothelin-3)(PPET3)
Species	Homo sapiens (Human)
Expression System	Yeast
Tag	C-6His-Myc
Target Protein Sequence	CTCFTYKDKECVYYCHLDIIW
Expression Range	97-117aa
Protein Length	Full Length of Mature Protein
Mol. Weight	7.0 kDa
Research Area	Cancer
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Endothelins are endothelium-derived vasoconstrictor peptides.
Subcellular Location	Secreted.
Protein Families	Endothelin/sarafotoxin family
Database References	HGNC: 3178 OMIM: 131242 KEGG: hsa:1908 STRING: 9606.ENSP00000337128 UniGene: PMID: 28880927 genome-wide association studies in population of women in China: Data suggest that EDN3 (endothelin 3) and EDNRB (endothelin receptor type B) play important roles in the molecular mechanisms underlying cervical cancer. PMID: 27863272 EDN3 expression in left internal mammary arteries depends on tissue harvesting technique. PMID: 24647318 Down-regulated expression of ET3 attenuates the malignant behaviors of human melanoma cells partially by decreasing the expression of SPARC. PMID: 23904381 Waardenburg syndrome type II and mutations of EDNRB, EDN3 and SOX10 genes are responsible for Waardenburg syndrome type IV. (review) PMID: 24379252 Our data showed that almost all patients, regardless of individual characteristics such as gender or age, expressed the endothelin receptor genes, but did not express the genes for ET-3. PMID: 23515723 These data suggest that autocrine EDN3/EDNRB signaling is essential for maintaining GSCs. Incorporating END3/EDNRB-targeted therapies into conventional cancer treatments may have clinical implication for the prevention of tumor recurrence. PMID: 22013079 Finding suggest that mutations in RET and NTRK3 acting together are necessary and sufficient for the appearance of Hirschsprung disease and that the EDN3 mutation acts as a phenotype modifier. PMID: 19556619 EDN3 may be considered as a common susceptibility gene for sporadic Hirschsprung disease in a low-penetrance fashion. PMID: 20009762 Mutations are found in Hirschsprung's disease in a Chinese population. PMID: 14669347 KEL6 red blood cells have endothelin-3-converting enzyme activity PMID: 16423827 neither polymorphism nor mutation was observed in EDN3 in Chinese Hirschprung disease patients PMID: 17554617 Endothelin-3 molecule is specifically upregulated in metastatic melanoma cells, showing that an abnormal autocrine stimulation pathway involving ET-3 is present in metastatic melanoma cells. PMID: 18346402 Endothelin signaling axis activates osteopontin expression through PI3 kinase pathway in A375 melanoma cells. PMID: 18722093 ET3 induced activation of IkappaB & MAPK in epithelial cells. ET3 is involved in regulating human colonic epithelial cell proliferation & survival, particularly for goblet cells. PMID: 18832450 In conclusion, Multiplex Ligation-dependent Probe Amplification assessment of rearrangements in the RET proto-oncogene and in 3 other associated genes, ZEB2, EDN3 and GDNF did not show any variants in 80 sporadic Hirschsprung disease patients. PMID: 19183406 Report on spanish cases of Waardenburg syndrome type 4 with novel mutations in EDN3 and SOX 10 genes. PMID: 19764030

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.