Recombinant Human eIF5A Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1744
Recombinant Human eIF5A Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1744
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Human |
Accession | NP_001961.1 |
Synonym | EIF-5A, EIF5A1, eIF5AI |
Background | EIF-5A, also known as EIF5, functions in start site selection as a GTPase accelerating protein (GAP) for the eukaryotic translation initiation factor (eIF) 2-€¢GTP-€¢tRNA ternary complex within the ribosome-bound pre-initiation complex. In protein synthesis initiation, eIF2 functions in its GTP-bound state to deliver initiator methionyl-tRNA to the small ribosomal subunit and is necessary for protein synthesis in all cells. EIF-5A stabilizes the binding of GDP to eIF2 and is therefore a bi-functional protein that acts as a GDP dissociation inhibitor (GDI). EIF-5A also interacts with eIF1 and eIF3 and binds the eIF2-GTP/Met-tRNA ternary complex along with the 4S ribosome subunit. |
Description | A DNA sequence encoding the human EIF5A (NP_001961.1) (Met1-Lys154) was expressed with a His tag at the N-terminus. |
Source | E.coli |
Predicted N Terminal | His |
AA Sequence | Met1-Lys154 |
Molecular Weight | The recombinant human EIF5A consists of 170 a.a. and predicts a molecular mass of 18.9 kDa. It migrates as an approximately 21 kDa band in SDS-PAGE under reducing conditions. |
Purity | >85% as determined by SDS-PAGE |
Endotoxin | Please contact us for more information. |
Bioactivity | Please contact us for detailed information |
Formulation | Lyophilized from sterile PBS, pH 7.4.. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |