Recombinant Human eIF5A Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1744
Recombinant Human eIF5A Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1744
Catalog No.: BLPSN-1744
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | NP_001961.1 |
| Synonym | EIF-5A, EIF5A1, eIF5AI |
| Background | EIF-5A, also known as EIF5, functions in start site selection as a GTPase accelerating protein (GAP) for the eukaryotic translation initiation factor (eIF) 2-€¢GTP-€¢tRNA ternary complex within the ribosome-bound pre-initiation complex. In protein synthesis initiation, eIF2 functions in its GTP-bound state to deliver initiator methionyl-tRNA to the small ribosomal subunit and is necessary for protein synthesis in all cells. EIF-5A stabilizes the binding of GDP to eIF2 and is therefore a bi-functional protein that acts as a GDP dissociation inhibitor (GDI). EIF-5A also interacts with eIF1 and eIF3 and binds the eIF2-GTP/Met-tRNA ternary complex along with the 4S ribosome subunit. |
| Description | A DNA sequence encoding the human EIF5A (NP_001961.1) (Met1-Lys154) was expressed with a His tag at the N-terminus. |
| Source | E.coli |
| Predicted N Terminal | His |
| AA Sequence | Met1-Lys154 |
| Molecular Weight | The recombinant human EIF5A consists of 170 a.a. and predicts a molecular mass of 18.9 kDa. It migrates as an approximately 21 kDa band in SDS-PAGE under reducing conditions. |
| Purity | >85% as determined by SDS-PAGE |
| Endotoxin | Please contact us for more information. |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile PBS, pH 7.4.. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
