Recombinant Human ECSIT Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1690
Recombinant Human ECSIT Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1690
Catalog No.: BLPSN-1690
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | Q9BQ95 |
| Synonym | SITPEC |
| Background | ECSIT is an adapter protein of the toll-like and IL-1 receptor signaling pathway that is involved in the activation of NF-kappa-B via MAP3K1. Activation of NF-kappaB as a consequence of signaling through the Toll and IL-1 receptors is a major element of innate immune responses. ECSIT is specific for the Toll/IL-1 pathways and is a regulator of MEKK-1 processing. It bridges TRAF6 to MEKK-1. Expression of wild-type ECSIT accelerates processing of MEKK-1, whereas a dominant-negative fragment of ECSIT blocks MEKK-1 processing and activation of NF-kappaB. ECSIT is also required for normal embryonic development and efficient assembly of mitochondrial NADH:ubiquinone oxidoreductase. |
| Description | A DNA sequence encoding the human ECSIT (Q9BQ95-1)(Glu246-Ser431) was expressed with a His tag at the N-terminus. |
| Source | E.coli |
| Predicted N Terminal | His |
| AA Sequence | Glu246-Ser431 |
| Molecular Weight | The recombinant human ECSIT consists of 201 a.a. and predicts a molecular mass of 22.9 KDa. It migrates as an approximately 25 KDa band in SDS-PAGE under reducing conditions. |
| Purity | >90% as determined by SDS-PAGE |
| Endotoxin | Please contact us for more information. |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile 50mM Tris, 10% Glycerol, pH 8.0.. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
