Recombinant Human ECD Protein (His & GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1683
Recombinant Human ECD Protein (His & GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1683
Catalog No.: BLPSN-1683
Product Overview
| Tag | His&GST |
| Host Species | Human |
| Accession | O95905 |
| Synonym | ECD, GCR2, HSGT1 |
| Background | ECD, also known as ecdysoneless homolog, belongs to the SGT1 family. It is highly expressed in muscle and heart. ECD is a novel promoter of mammalian cell cycle progression. This function is related to its ability to remove the repressive effects of Rb-family tumor suppressors on E2F transcription factors. It is a novel tumor-promoting factor that is differentially expressed in pancreatic cancer and potentially regulates glucose metabolism within cancer cells. ECD may also be a transcriptional activator required for the expression of glycolytic genes. |
| Description | A DNA sequence encoding the human ECD (O95905-1) (Met1-Thr621) was expressed with the N-terminal His-tagged GST tag at the N-terminus. |
| Source | Baculovirus-Insect Cells |
| Predicted N Terminal | Met |
| AA Sequence | Met1-Thr621 |
| Molecular Weight | The recombinant human ECD/GST chimera consists of 881 a.a. and has a calculated molecular mass of 100.6 kDa. The recombinant protein migrates as an approximately 88-108 kDa band in SDS-PAGE under reducing conditions. |
| Purity | >90% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4.. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
