Recombinant Human Dynactin Subunit 1 (DCTN1) Protein (His-SUMO)

Name: Recombinant Human Dynactin Subunit 1 (DCTN1) Protein (His-SUMO)
Brand: Beta LifeScience
SKU: BLC-02547P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Human Dynactin Subunit 1 (DCTN1) Protein (His-SUMO) is produced by our E.coli expression system. This is a protein fragment.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	Q14203
Target Symbol	DCTN1
Synonyms	150 kDa dynein associated polypeptide; 150 kDa dynein-associated polypeptide; DAP 150; DAP-150; DAP150; DCTN 1; DCTN1; DCTN1_HUMAN; DP 150; DP-150; DP150; Dynactin 1 (p150 Glued (Drosophila) homolog); Dynactin 1 (p150 glued homolog Drosophila); Dynactin 1; Dynactin subunit 1; Dynactin1; HMN7B; p135; p150 Glued (Drosophila) homolog ; p150 glued; p150 glued homolog; p150(GLUED) DROSOPHILA HOMOLOG OF; p150-glued; p150glued
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His-SUMO
Target Protein Sequence	PSKEEEGLRAQVRDLEEKLETLRLKRAEDKAKLKELEKHKIQLEQVQEWKSKMQEQQADLQRRLKEARKEAKEALEAKERYMEEMADTADAIEMATLDKEMAEERAESLQQEVEALKERVDELTTDLEILKAEIEEKGSDGAASSYQLKQLEEQNARLKDALVRMRDLSSSEKQEHVKLQKLMEKKNQELEVVRQQRERLQEELSQAESTIDELKEQVDAALGAEEMVEMLTDRNLNLEEKVRELRETVGDLEAMNEMNDELQENARETELELREQLDMAGARVREAQKRVEAAQETVADYQQTIKKYRQLTAHLQDVNRELTNQQEASVERQQQ
Expression Range	213-547
Protein Length	Partial
Mol. Weight	55.2kDa
Research Area	Cell Biology
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Plays a key role in dynein-mediated retrograde transport of vesicles and organelles along microtubules by recruiting and tethering dynein to microtubules. Binds to both dynein and microtubules providing a link between specific cargos, microtubules and dynein. Essential for targeting dynein to microtubule plus ends, recruiting dynein to membranous cargos and enhancing dynein processivity (the ability to move along a microtubule for a long distance without falling off the track). Can also act as a brake to slow the dynein motor during motility along the microtubule. Can regulate microtubule stability by promoting microtubule formation, nucleation and polymerization and by inhibiting microtubule catastrophe in neurons. Inhibits microtubule catastrophe by binding both to microtubules and to tubulin, leading to enhanced microtubule stability along the axon. Plays a role in metaphase spindle orientation. Plays a role in centriole cohesion and subdistal appendage organization and function. Its recruitment to the centriole in a KIF3A-dependent manner is essential for the maintenance of centriole cohesion and the formation of subdistal appendage. Also required for microtubule anchoring at the mother centriole. Plays a role in primary cilia formation.
Subcellular Location	Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cytoplasm, cytoskeleton, spindle. Nucleus envelope. Cytoplasm, cell cortex.
Protein Families	Dynactin 150 kDa subunit family
Database References	HGNC: 2711 OMIM: 105400 KEGG: hsa:1639 STRING: 9606.ENSP00000354791 UniGene: PMID: 28625595 ASK1- induced phosphorylation of EB1 not only increases its plus end-tracking ability, but also promotes its recruitment of CLIP170 and p150glued to astral microtubules. PMID: 28039481 DCTN1 p.K56R in patients with PSP. PMID: 27132499 Results suggested that variants in DCTN1 are not common risk factors for Chinese sporadic amyotrophic lateral sclerosis and that the frequency of variants of unknown significance in the cohort study was 0.39%. PMID: 28792508 This work reveals the structural details of Hook3's interaction with dynein and offers insight into how cargo adaptors form processive dynein-dynactin motor complexes. PMID: 27482052 No DCTN1 variants of disease significance were identified in this study suggesting the dynactin genes are unlikely to be a common cause of inherited peripheral neuropathies. PMID: 26662454 Mutations in the Dynactin 1 (DCTN1) gene have been demonstrated to result in various neurodegenerative diseases, including distal hereditary motor neuropathy type 7B. In the present study, a p.G59S mutation was identified as the underlying cause of Distal hereditary motor neuropathy type 7B in two families, and their detailed clinical features were characterized. PMID: 27573046 Study found that in peripheral blood mononuclear cells the median expression of KIFC3, KIF1B, and KIF5C was much lower than the expression of dynactin subunits DCTN1 and DCTN3, in both sporadic amyotrophic lateral sclerosis and healthy cases PMID: 26954557 Data suggest that cargo concentration at ERES is regulated by p150(glued) to coordinate protein sorting and transport carrier formation with the subsequent long-range transport towards the Golgi complex along microtubules. PMID: 26459637 Study shows p150glued located at the centrosome in a cell cycle-dependent manner where it is abundant during G1/S phase, located in the minus-end of microtubules during G2/M phase and at the minus-end of microtubules in the mitotic phase. PMID: 25774020 We find that LRRK1-mediated phosphorylation of CLIP-170 causes the accumulation of p150(Glued) (also known as DCTN1) a subunit of dynactin, at microtubule plus ends, thereby facilitating the migration of EGFR-containing endosomes. PMID: 25413345 The results describe the mutant dynactin p150Glued mouse model of motor neuron disease and show that genetic background influences phenotype in part through a region of chromosome 17 supporting the presence of genetic modifiers. PMID: 25763819 Dynactin functions as both a dynamic tether and brake during dynein-driven motility. PMID: 25185702 HPS6 interacts with dynactin p150Glued to mediate retrograde trafficking and maturation of lysosomes PMID: 25189619 Taken together, topographic and functional interactions between dynactin, importin-beta and RanBP2 are involved in nuclear translocation of IGF-1R. PMID: 24909165 DCTN1 mutations linked with neurogeneration on Guam. PMID: 25558820 The results of this study DCTN1 mutations may contribute to disparate neurodegenerative diagnoses, including familial motor neuron disease, parkinsonism, and frontotemporal atrophy. PMID: 24676999 The data of this study showed evidence of both striatal dopaminergic and widespread cortical/subcortical serotonergic dysfunctions in individuals carrying a mutation in the DCTN1 gene. PMID: 24797316 We identified 2 novel mutations of DCTN1 in patients with Perry syndrome PMID: 24484619 The fact that SLK (LOSK) phosphorylates only a minor isoform 1A of p150(Glued) suggests that transport and microtubule-organizing functions of dynactin are distinctly divided between the two isoforms PMID: 23985322 A DCTN1 point mutation was identified in a large family with progressive supranuclear palsy. PMID: 24343258 Data find that dynactin has multiple functions in neurons, both activating dynein-mediated retrograde axonal transport and enhancing microtubule stability through a novel anti-catastrophe mechanism regulated by tissue-specific isoform expression PMID: 23874158 The results of this study suggested the presence of biological changes caused by some p150 mutants pointing to a potential pathogenetic significance as modifier of the phenotype of the human disease. PMID: 23143281 Aurora A is involved in central spindle assembly through phosphorylation of Ser 19 in P150Glued. PMID: 23547029 in addition to its ciliogenic roles, Kif3a recruits p150(Glued) to the subdistal appendages of mother centrioles, critical for centrosomes to function as microtubule-organizing centres. PMID: 23386061 LIS1 is required for dynein-mediated transport induced by membrane tethering of BICD2-N and LIS1 contributes to dynein accumulation at microtubule plus ends and BICD2-positive cellular structures. PMID: 22956769 Overexpression of TBCB leads to the decreased localization of p150 to the microtubule network that might result in a functional modulation of this protein complex. PMID: 22777741 The tubule formations were dependent on microtubule interactions, and specifically controlled by Kif16b and dynein 1. PMID: 22357949 mTORC1 activation requires dynein-dependent transport to a position in the cell where it can be activated PMID: 22987636 Data suggest that TRAPPC9 serves to uncouple p150(Glued) from the COPII coat, and to relay the vesicle-dynactin interaction at the target membrane. PMID: 22279557 The p150(Glued) CAP-Gly domain regulates dynein-mediated retrograde transport at synaptic termini, and this function of dynactin is disrupted by a mutation that causes motor neuron disease. PMID: 22542187 End-binding proteins interact with the CAP-Gly domains of CLIP-170 and p150(glued). PMID: 22119847 Studies indicate that binding of dynactin, LIS1 and NudEL regulate cytoplasmic dynein motor activity. PMID: 22373868 Endosome movement requires an intact dynactin complex to allow p150(Glued) to activate dynein, since p50 over-expression, which disrupts the dynactin complex, inhibits inward movement even though dynein and p150(Glued) remain membrane-bound. PMID: 21915335 DCTN1 mutation in patients with with early stage of Perry syndrome presented with marked autonomic dysfunction including orthostatic hypotension and decreased cardiac uptake with [123]I-metaiodobenzylguanidine scintigram features PMID: 20702129 Par6alpha controls centrosome organization through its association with the dynactin subunit p150(Glued). PMID: 20719959 Data show that polo-like kinase 1 phosphorylation of p150(Glued) might be one major pathway of NEBD regulation. PMID: 20679239 study describes a new family carrying a G71R mutation in the DCTN1 gene; the proband displayed a series of distinctive features not previously described in Perry syndrome PMID: 20437543 demonstrate that the domains harboring mutated CAP-gly domains bind to microtubules but fail to bind to EB1 PMID: 20518521 Disruption of the SNX6-p150(Glued) interaction causes failure in formation and detachment of the tubulovesicular sorting structures from endosomes and results in block of CI-MPR retrieval from endosomes to the TGN. PMID: 19935774 DCTN1 subunit p150Glued isoforms notable for differential interaction with microtubules. PMID: 19778315 Genomic organization of the DCTN1-SLC4A5 locus encoding both NBC4 and p150(Glued). PMID: 12063394 dysfunction of dynactin-mediated transport can lead to human motor neuron disease PMID: 12627231 neither APC nor p150glued binding domain is necessary for EB1 or EBF3 to induce microtubule bundling PMID: 14514668 role in extension and assembly of adherens junctions in photoreceptor development PMID: 15067220 Heterozygous missense mutations of DCTN1 were found in a sporadic case of ALS (T1249I), 1 pt with familial ALS (M571T), two pts with familial ALS, & two unaffected relatives (R785W). Alleleic variants of DCTN1 may be a genomic risk factor for ALS. PMID: 15326253 The R1101K sequence alteration of the DCTN1 gene may predispose subjects to ALS and FTD. PMID: 16240349 The Gly59Ser mutation disrupts folding of the cytoskeleton-associated protein glycine-rich (CAP-Gly) domain, resulting in aggregation of p150Glued protein in vitro and in vivo, accompanied by an increase in cell death in a motor neuron cell line. PMID: 16505168 The function of p150(Glued) in membrane trafficking is not associated with plus-end localization. PMID: 16772339 Dynactin p150(Glued) plays an important role in the functional integrity of the keratinocyte microparasol. PMID: 17344930

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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