Recombinant Human DUSP3 Protein
Beta LifeScience
SKU/CAT #: BLPSN-1668
Recombinant Human DUSP3 Protein
Beta LifeScience
SKU/CAT #: BLPSN-1668
Catalog No.: BLPSN-1668
Product Overview
| Tag | N/A |
| Host Species | Human |
| Accession | P51452 |
| Synonym | VHR |
| Background | Vaccinia H1-related phosphatase (VHR) is classified as a dual-specificity phosphatase (DUSP), and the other name is dual-specificity phosphatase 3 (DUSP3). DUSPs are a heterogeneous group of protein phosphatases that can dephosphorylate both phosphotyrosine and phosphoserine/phosphothreonine residues within the one substrate. Unlike typical DUSPs, VHR lacks mitogen-activated protein kinase (MAPK)-binding domain, and shows poor activity against MAPKs. VHR often act on bisphosphorylated protein substrates, it displays a strong preference for dephosphorylating phosphotyrosine residues over phosphothreonine residues. VHR has been identified as a novel regulator of extracellular regulated kinases (ERKs). VHR is responsible for the rapid inactivation of ERK following stimulation and for its repression in quiescent cells. VHR is a negative regulator of the Erk and Jnk pathways in T cells and, therefore, may play a role in aspects of T lymphocyte physiology that depend on these kinases. |
| Description | A DNA sequence encoding the human VHR (P51452)(Met 1-Pro 185)was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus. |
| Source | Baculovirus-Insect Cells |
| Predicted N Terminal | Gly |
| AA Sequence | Met 1-Pro 185 |
| Molecular Weight | The secreted recombinant human VHR consists of 187 a.a. and predicts a molecular mass of 20.6 KDa. The apparent molecular mass of the protein is approximately 21 KDa in SDS-PAGE under reducing conditions due to glycosylation. |
| Purity | >95% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by hydrolysis of 250 µM 3-O-methyl fluorescein phosphate (OMFP).The specific activity is >1.0 µmol/min/mg. |
| Formulation | Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0.. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
