Recombinant Human DPEP1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1643
Recombinant Human DPEP1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1643
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Human |
Accession | P16444 |
Synonym | MBD1, MDP, RDP |
Background | Dehydropeptidase-I, also known as DPEP1, is a kidney membrane enzyme. Its expression in normal colonic mucosa is very low, but it is highly expressed in colorectal adenoma and cancer specimens and is negatively correlated with parameters of pathological aggressiveness and poor prognosis. The overexpression of DPEP1 suppressed tumor cells invasiveness and increased sensitivity to chemotherapeutic agent Gemcitabine. Growth factor EGF treatment decreased DPEP1 expression. Dehydropeptidase-I may be a candidate target in PDAC for designing improved treatments. It uses zinc as a cofactor and acts as a disulfide-linked homodimer. |
Description | A DNA sequence encoding the human DPEP1 (P16444) (Met1-Ser385) was expressed with a His tag at the C-terminus. |
Source | HEK293 |
Predicted N Terminal | Asp 17 |
AA Sequence | Met1-Ser385 |
Molecular Weight | The recombinant human DPEP1 consists of 380 a.a. and predicts a molecular mass of 42.5 KDa. It migrates as an approximately 44-47 KDa band in SDS-PAGE under reducing conditions. |
Purity | >95% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Please contact us for detailed information |
Formulation | Lyophilized from sterile PBS, pH 7.4. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Target Details
Target Function | Hydrolyzes a wide range of dipeptides including the conversion of leukotriene D4 to leukotriene E4. Hydrolyzes cystinyl-bis-glycine (cys-bis-gly) formed during glutathione degradation. Possesses also beta lactamase activity and hydrolytically inactivates beta-lactam antibiotics.; Independently of its dipeptidase activity acts as an adhesion receptor for neutrophils recruitement from bloodstream into inflammed lungs and liver. |
Subcellular Location | Apical cell membrane; Lipid-anchor, GPI-anchor. Cell projection, microvillus membrane; Lipid-anchor, GPI-anchor. |
Protein Families | Metallo-dependent hydrolases superfamily, Peptidase M19 family |
Database References | |
Tissue Specificity | Expressed in lung and kidneys. |
Gene Functions References
- The results suggest that DPEP1 promotes cancer metastasis by regulating E-cadherin plasticity and that it might be a potential therapeutic target for preventing the progression of colon cancer. PMID: 26824987
- In this study, we present an analysis of Neanderthal introgression at the dipeptidase 1 gene, DPEP1. PMID: 26392408
- Dipeptidase 1 has been identified as an excellent marker of high-grade IEN and CRC, and may thus be applied for screening of early neoplastic lesions and for prognostic stratification. PMID: 23839495
- DPEP1 plays a role in pancreatic cancer aggressiveness. PMID: 22363658
- DPEP1 is expressed in the early stages of colon carcinogenesis and affects cancer cell invasiveness. PMID: 20824289
- we identified a novel immunohistochemical marker, dipeptidase 1, to distinguish primary mucinous ovarian cancers from ovarian metastasis of colorectal cancers. PMID: 21076463
- Crystal structure of human renal dipeptidase involved in beta-lactam hydrolysis PMID: 12144777
- DPEP1 has a role in colorectal carcinoma PMID: 15145522