Recombinant Human Doublecortin / DBCN Protein (aa 45-150, GST Tag)

Name: Recombinant Human Doublecortin / DBCN Protein (aa 45-150, GST Tag)
Brand: Beta LifeScience
SKU: BLPSN-1642
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Tag	GST
Host Species	Human
Accession	O43602
Synonym	DBCN, DC, LISX, SCLH, XLIS
Background	DCX (doublecortin, N-GST chimera)contains 2 doublecortin domains and belongs to the doublecortin family. It is highly expressed in neuronal cells of fetal brain, but not expressed in other fetal tissues. In the adult, it is highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas. DCX is a microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. It may act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. DCX may in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. It may be part with LIS-1 of a overlapping, but distinct, signaling pathways that promote neuronal migration. Defects in DCX are the cause of lissencephaly X-linked type 1 and subcortical band heterotopia X-linked.
Description	A DNA sequence encoding the human DCX (O43602-2) N-terminal fragment (Ala 45-Val 150) was fused with the GST tag at the N-terminus.
Source	E.coli
Predicted N Terminal	Met
AA Sequence	Ala 45-Val 150
Molecular Weight	The recombinant human DCX (aa 45-150)/GST chimera consists of 340 a.a. and has a predicted molecular mass of 39.4 kDa. It migrates as an approxiamtely 36 KDa band in SDS-PAGE under reducing conditions.
Purity	>82% as determined by SDS-PAGE
Endotoxin	Please contact us for more information.
Bioactivity	Please contact us for detailed information
Formulation	Lyophilized from sterile 20mM Tris, 1mM DTT, 10% glycerol, pH 7.5.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration.
Subcellular Location	Cytoplasm. Cell projection, neuron projection.
Database References	HGNC: 2714 OMIM: 300067 KEGG: hsa:1641 STRING: 9606.ENSP00000337697 UniGene: Hs.34780
Associated Diseases	Lissencephaly, X-linked 1 (LISX1); Subcortical band heterotopia X-linked (SBHX)
Tissue Specificity	Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low ex

Gene Functions References

Age-associated expression of the proliferation and immature neuron markers MKI67 and DCX, respectively, was unrelated, suggesting that neurogenesis-associated processes are independently altered at these points in the development from stem cell to neuron. PMID: 28766905
specifically recognizes compacted GDP-like microtubule lattice PMID: 27238282
this suggests that the microtubule-interacting doublecortin domain observed in cryo-electron micrographs is the C-terminal domain rather than the N-terminal one. PMID: 27226599
From this family, we conclude that a DCX mutation causes a pleiotropic phenotype in the female even if X chromosome inactivation pattern is not skewed, and the novel missense mutation in DCX produced relatively mild dysfunction of the doublecortin protein. PMID: 25868952
We identified a novel DCX mutation c.785A > G, p.Asp262Gly in a family with X-linked lissencephaly and subcortical band heterotopia PMID: 27292316
In high-risk metastatic Neuroblastoma, TH and DCX mRNA quantification could be used for the assessment of response to treatment and for early detection of progressive disease or relapses. PMID: 26498952
Results point to a critical role of doublecortin in the formation of the neuromuscular junctions. PMID: 25817838
It was conclude that microtubule ends have two distinct features that proteins can recognize independently, namely a structural feature related to curvature and nucleotide state. PMID: 25283777
DCX-positive cells occur in a wide range of hypothalamic nuclei in humans, mice and sheep. PMID: 24288185
human DCX protein was expressed in human adipose stem cells, collagen II was decreased while aggrecan, matrilin 2, and GDF5 were increased during the 14-day pellet culture. PMID: 24758934
We propose that DCDC2 is a tumor suppressor gene of HCC. PMID: 24034596
in utero doublecortin knockdown, but not knockout, shows a neocortical neuronal migration phenotype. PMID: 24945770
Immunoblots revealed that depressed subjects displayed increased expression of doublecortin. PMID: 23260340
coexpression of DCX and SPARC collaboratively diminished radioresistance of glioma cells. PMID: 23846421
DCX is dispensable for the development of new neurons in adult mice PMID: 23667508
one deleterious mutation in the DCX gene was identified in a 5-year-old girl with lissencephaly spectrum PMID: 23583063
This finding points to the possible implication of mosaic deletions in the DCX gene in unexplained forms of subcortical band heterotopia (SBH) and may allow for detection of SBH prenatally. PMID: 22833188
DCX binds to polymerization intermediates at growing microtubule ends, in support of a mechanism for stabilizing 13-protofilament microtubules. PMID: 22727374
Data report a negative correlation between DCX mRNA expression and white matter neuron density in schizophrenia, in contrast to a positive correlation in human development where DCX mRNA and white matter neuron density are higher earlier in life. PMID: 21966452
Up-regulation of doublecortin is associated with brain damage in children with acute lymphoblastic leukemia PMID: 21846577
DCX synthesis induces apoptosis in brain tumor stem cells (BTSC) through a novel JNK1/neurabin II/DCX/PP1/caspase-3 pathway. PMID: 21477071
study describes male siblings with Lennox-Gastaut syndrome and pachygyria with a novel missense mutation in the DCX gene PMID: 20726879
The DCX binding on the microtubules surface indirectly stabilizes conserved tubulin-tubulin lateral contacts in the microtubules lumen, operating independently of the nucleotide bound to tubulin. PMID: 20974813
variants in doublecortin- and calmodulin kinase like 1, a gene up-regulated by BDNF, have roles in memory and general cognitive abilities PMID: 19844571
Evidence for a role for miR-128 in neuroblastoma progression and aggressiveness through reelin and DCX expression is reported. PMID: 19713529
During development of the cerebral cortex DCX is expressed in both pyramidal and non-pyramidal migrating cells, as well as in Cajal-Retzius cells. PMID: 12427674
Missense DCX mutations may manifest as non-syndromic mental retardation with cryptogenic epilepsy in female subjects and subcortical band heterotopia (SBH) in boys. PMID: 12838518
Different clinical and morphological phenotypes in monozygotic twins with identical DCX mutation. PMID: 14999500
both mutant and wild type DCX are able to bind and bundle microtubules; however, mutants possess a decreased ability to perturb the mitotic machinery, to cause abnormal spindle orientation, and to impair mitotic progression PMID: 15045646
DCX maps at Xq22.3 and is caused by a homozygous mutation. It acts during corticogenesis on radial migratory pathways. PMID: 15057976
We analysed the human DCX regulatory sequence and confined it to a 3.5-kb fragment upstream of the ATG start codon. This fragment is sufficient and specific to drive expression of reporter genes in embryonic and adult neuronal precursors PMID: 15663475
doublecortin (DCX) gene as a new molecular marker of neuroblastoma cells PMID: 15714065
Collectively, the immunohistochemistry, Western blots and Northern blots conclusively demonstrate expression of DCX by human brain tumors. PMID: 16195916
Doublecortin can be regarded as specific neuronal marker only in normal developing brain, but lacks specificity in glioneuronal and glial tumours and other non-neuronal human tissues where it is expressed in a wide variety of tumours and tissues. PMID: 16520969
X-ray structure of a mutant of N-DCX, in which the C-terminal fragment (residues 139-147) unexpectedly shows an altered, "open" conformation PMID: 16835924
Our data in the mouse, identifying roles for Dcx in hippocampal and corpus callosal development, might suggest intrinsic roles for human DCX in the development of these structures. PMID: 17111359
Doublecortin (DCX) is one of the three genes found from Affymetrix gene chip analysis related to glioma patient survival. PMID: 17178868
MLPA uncovers large genomic deletions of the DCX gene in a subset of patients with SBH in whom no mutations are found after gene sequencing. Deletions of DCX are an underascertained cause of SBH. PMID: 17283321
Results demonstrate that DCX can be used as a marker to distinguish articular chondrocytes from other chondrocytes and to evaluate the quality of tissue engineered or regenerated cartilage in terms of their "articular" or "non-articular" nature. PMID: 17897623
Doublecortin is applicable for the detection of individual infiltrating glioma cells when combined with other markers. PMID: 18415660
X-linked lissencephaly patients with mutations in the C-DC domain tended to have a less severe lissencephaly (grade 4-5 in 58.3%) compared with those in the N-DC domain (grade 4-5 in 36.3%. PMID: 18685874
intragenic deletions and duplications of the DCX gene account for a significant number of patients with isolated lissencephaly sequence and subcortical band heterotopia, where no molecular defect had previously been identified. PMID: 19050731
Doublecortin (DCX) upregulation, correlates with better neurologic outcome in children following traumatic head injury. PMID: 19221293

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