Recombinant Human DOT1L (KMT4) Protein

Beta LifeScience SKU/CAT #: BL-0301SG
Recombinant Human DOT1L (KMT4) Protein

Recombinant Human DOT1L (KMT4) Protein

Beta LifeScience SKU/CAT #: BL-0301SG
Catalog No.: BL-0301SG

Product Overview

Tag GST
Host Species Human
Accession NM_032482
Synonym DOT1; KMT4
Background DOT1-like protein (DOT1L) specifically methylates lysine-79 of histone H3 in the globular domain. DOT1L lacks a SET-domain, which is commonly considered to mediate lysine methylation of histones (1, 2). DOT1 homologs exist in a variety of eukaryotic organisms. The activity of this methyltransferase is involved in cell cycle regulation and leukemogenesis (3).
Description Recombinant human DOT1L (KMT4) (1-435) was produced by baculovirus in Sf9 insect cells, fused with a GST tag at N-terminus. This protein is purified with our unique purification methods.
Source Sf9 insect cells
AA Sequence 1-435
Molecular Weight 78 kDa
Purity For specific purity information on a given lot, see related COA.
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Active
Formulation Recombinant protein is supplied in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol.
Stability The recombinant protein is stable for up to 12 months at -70°C
Usage For Research Use Only
Storage Recombinant Human DOT1L (KMT4) Protein should be stored should be stored at < -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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