Recombinant Human DOT1L (KMT4) Protein
Beta LifeScience
SKU/CAT #: BL-0301SG
Recombinant Human DOT1L (KMT4) Protein
Beta LifeScience
SKU/CAT #: BL-0301SG
Catalog No.: BL-0301SG
Product Overview
| Tag | GST |
| Host Species | Human |
| Accession | NM_032482 |
| Synonym | DOT1; KMT4 |
| Background | DOT1-like protein (DOT1L) specifically methylates lysine-79 of histone H3 in the globular domain. DOT1L lacks a SET-domain, which is commonly considered to mediate lysine methylation of histones (1, 2). DOT1 homologs exist in a variety of eukaryotic organisms. The activity of this methyltransferase is involved in cell cycle regulation and leukemogenesis (3). |
| Description | Recombinant human DOT1L (KMT4) (1-435) was produced by baculovirus in Sf9 insect cells, fused with a GST tag at N-terminus. This protein is purified with our unique purification methods. |
| Source | Sf9 insect cells |
| AA Sequence | 1-435 |
| Molecular Weight | 78 kDa |
| Purity | For specific purity information on a given lot, see related COA. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Active |
| Formulation | Recombinant protein is supplied in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol. |
| Stability | The recombinant protein is stable for up to 12 months at -70°C |
| Usage | For Research Use Only |
| Storage | Recombinant Human DOT1L (KMT4) Protein should be stored should be stored at < -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
