Recombinant Human DMP1 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-1634

Recombinant Human DMP1 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-1634
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Product Overview

Tag His
Host Species Human
Accession Q13316
Synonym ARHP, ARHR, DMP-1
Background Dentin matrix acidic phosphoprotein (DMP1) is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. DMP1 contains a large number of acidic domains, multiple phosphorylation sites, a functional arg-gly-asp cell attachment sequence, and a DNA binding domain. In undifferentiated osteoblasts it is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation, DMP1 becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in DMP1 are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. DMP1 may have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite.
Description A DNA sequence encoding the human DMP1 (Q13316-1) (Met 1-Tyr 513) was expressed, with a His tag at the C-terminus.
Source HEK293
Predicted N Terminal Leu 17
AA Sequence Met 1-Tyr 513
Molecular Weight The recombinant human DMP1 consists of 508 a.a. and predictes a molecular mass of 55.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhDMP1 is approximately 53 kDa.
Purity >85% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Measured by its ability to bind human CFH in a functional ELISA.
Formulation Lyophilized from sterile PBS, pH 7.4.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function May have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite.
Subcellular Location Nucleus. Cytoplasm. Secreted, extracellular space, extracellular matrix. Note=In proliferating preosteoblasts it is nuclear, during early maturation stage is cytoplasmic and in mature osteoblast localizes in the mineralized matrix. Export from the nucleus of differentiating osteoblast is triggered by the release of calcium from intracellular stores followed by a massive influx of this pool of calcium into the nucleus.
Database References
Associated Diseases Hypophosphatemic rickets, autosomal recessive, 1 (ARHR1)
Tissue Specificity Expressed in tooth particularly in odontoblast, ameloblast and cementoblast.

Gene Functions References

  1. dentin matrix protein 1 was detected in the periodontal ligament co-localized with periostin in the vicinity of the cement PMID: 29289709
  2. The Dentin matrix protein1 (Dmp1) is an acidic phosphoprotein of osteocytes proposed to participate in bone and dentin mineralization. PMID: 28247029
  3. Thus, unspDMP-1 is a novel chromatin-enriched RNA that epigenetically regulates cellular proliferation of SQCC. PMID: 29278708
  4. DMP1 might be a potential factor contributing to cardiovascular complications in dialysis patients. PMID: 27390906
  5. DMP1 gene seems to be involved in genetic predisposition to Ankylosing spondylitis (AS). PMID: 26960368
  6. DMP-1 immunostaining was higher for MTA and PC, confirming the reparative and bioinductive capacity of these materials. PMID: 25678029
  7. This review will focus on the aberrant splicing of tumor suppressor/oncogenes that belong to the DMP1-ARF-MDM2-p53 pathway PMID: 26802432
  8. Expressions of dentin matrix protein (DMP)-1 and osteopontin (OPN) were observed in both normal dentin and dentin from DGI-I affected patients, without significant differences PMID: 25578972
  9. identify alternative splicing as a mechanism utilized by cancer cells to modulate the DMP1 locus through diminishing DMP1alpha tumour suppressor expression PMID: 25537728
  10. The findings indicate that DMP-1 is a useful marker of osteogenic differentiation and mineralisation in soft tissue tumours PMID: 25630512
  11. A family of autosomal recessive form of Hypophosphatemic rickets secondary to a DMP1 mutation located in the first coding exon of the gene, is reported. PMID: 25180662
  12. Results suggest that FAM20C suppresses FGF23 production by enhancing DMP1 expression, and inactivating mutations in FAM20C cause FGF23-related hypophosphatemia by decreasing transcription of DMP1. PMID: 25026495
  13. DMP1 may play an important role in maintaining the chondrogenic phenotype and its possible involvement in altered cartilage matrix remodelling and degradation in disease conditions like osteoarthritis. PMID: 24987156
  14. DMP1 and DSPP were more abundant in carious than in sound samples. PMID: 24441185
  15. DMP-1 is a matrix marker expressed around osteocytes in human woven and lamellar bone and is useful in identifying osteosarcoma and other bone-forming tumours. PMID: 23559304
  16. Mutations in PHEX and DMP1 play a role in causing hypophosphatemic rickets. PMID: 22695891
  17. DMP1 is regulated post-transcriptionally by let-7 during odontoblast differentiation. PMID: 22552299
  18. DMP-1 stimulated production of IL-6/IL-8 from pulp fibroblasts. Inhibition of p38 mitogen-activated protein kinase pathway blocked proinflammatory effect on pulp fibroblasts. DMP-1 might participate in development of inflammatory changes in pulp. PMID: 22152625
  19. Simvastatin plus all-trans retinoic acid reduces expression of WT1 and DMP1 in the promyelocytic leukemia cell line NB4. PMID: 22093791
  20. DMP1 is specifically involved in signaling via extracellular matrix-cell surface interaction. PMID: 21642437
  21. Data show the wide spectrum of genetic variation that can be seen in PHEX, FGF23 and DMP1 when screening a large cohort with hypophosphatemic rickets. PMID: 21050253
  22. after VE-cadherin induction by DMP1, DMP1 inhibited VEGFR-2 phosphorylation and Src-mediated signaling; DMP1 is identified as a new specific inhibitor of VEGF-induced angiogenesis PMID: 21190990
  23. possible role of dentine matrix protein 1 in the aetiology of [aggressive periodontitis] PMID: 21299946
  24. clinical and laboratory observations in this family confirm that DMP1 has an important role in normal skeletal development and mineral homeostasis PMID: 20499351
  25. Study shows the pivotal roles of Dmp1 in HER2/neu-p53 signaling and breast carcinogenesis. PMID: 21062982
  26. study describes a Japanese family that includes 2 autosomal recessive hypophosphatemic rickets-affected siblings carrying a novel mutation of the DMP1 gene (98G>A, W33X), which leads to a truncated DMP protein with no putative biological function PMID: 20213538
  27. The identified genetic mutation underscores the importance of DMP-1 mutations in the pathogenesis of autosomal recessive form of hypophosphatemia. PMID: 19796717
  28. The patterns of expression of FGF-23, MEPE, and DMP1 differ markedly in trabecular bone, suggesting that individual osteocytes may have specialized functions. PMID: 19679205
  29. Three SIBLINGs (small integrin-binding ligand, N-linked glycoproteins) enhance factor H's cofactor activity enabling MCP-like cellular evasion of complement-mediated attack. PMID: 11825898
  30. DMP1 immunostaining intensity and extent scores were significantly higher in adenocarcinoma (p = 0.0004) and squamous carcinoma (p < 0.0001) samples compared with adjacent normal lung tissue PMID: 12929940
  31. dentin matrix protein-1 is processed by Bone morphogenetic protein-1/Tolloid-like proteinases PMID: 14578349
  32. Findings indicate that DMP1 immunohistochemistry is a useful tool for identifying phosphaturic mesenchymal tumors (PMTs). PMID: 15001995
  33. a silencing cis-element may play a critical role in the regulation of DMP1 cell-specific expression PMID: 15108359
  34. 8-kb region of the Dentin matrix protein 1 (DMP1) gene is a target for mechanotransduction in osteocytes, and its cis-regulatory activity may be correlated to local strain in bone PMID: 15728181
  35. Dentin matrix protein 1 enhances invasion potential of colon cancer cells by bridging matrix metalloproteinase-9 to integrins and CD44 PMID: 16357164
  36. analysis of a chondroitin sulfate chain attached to the bone dentin matrix protein 1 NH2-terminal fragment PMID: 16421105
  37. DMP1 regulates the expression of the DSPP gene PMID: 16679514
  38. Absence of DMP1 results in defective osteocyte maturation and increased FGF23 expression, leading to pathological changes in bone mineralization. PMID: 17033621
  39. DMP1 may regulate FGF23 expression. PMID: 17033625
  40. DMP1 has a role in breast cancer progression and bone metastases development PMID: 17136477
  41. Thus, our results suggest that Cbfalpha1 upregulates DMP1 gene expression differentially that may contribute to the spatial-temporal expression pattern of DMP1 during odontoblast differentiation. PMID: 17434448
  42. Discussion of DMP1 role in the maturation of odontoblasts and osteoblasts as well as in mineralization; identification of DMP1 mutations in autosomal-recessive hypophosphatemic rickets; and regulation of phosphate homeostasis through FGF23 [review]. PMID: 18037646
  43. mRNA of DMP1 involved in the pathogenesis of hypophosphataemic rickets is highly expressed in cells of the osteoblasts/osteocyte lineage. PMID: 18214537
  44. inactivating mutations in DMP1 increase, through yet unknown mechanisms, FGF23 synthesis and thus enhance renal phosphate excretion--REVIEW PMID: 18660670
  45. binding of DMP1 with GRP-78 receptor might be an important mechanism by which DMP1 is internalized and transported to the nucleus during bone and tooth development PMID: 18757373
  46. Dentin matrix protein-1 isoforms promote differential cell attachment and migration PMID: 18819913
  47. A novel DMP1 deletion is identified as the cause of autosomal recessive hypophosphatemic rickets, as well as demonstrated that the ARHR mutations alter DMP1 cellular processing, and that DMP1 can be regulated by vitamin D. PMID: 19007919
  48. Deletion of mouse Dmp1 results in bone hypomineralization. PMID: 16294270
  49. DMP1 is expressed in salivary gland ducts. PMID: 15329369
  50. DMP1 is expressed in parietal cells of Bowman's capsule and throughout the ductal system of nephrons. PMID: 15954904


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