Recombinant Human Deoxynucleotidyltransferase Terminal-Interacting Protein 1 (DNTTIP1) Protein (His)
Beta LifeScience
SKU/CAT #: BLC-10243P

Greater than 90% as determined by SDS-PAGE.
Recombinant Human Deoxynucleotidyltransferase Terminal-Interacting Protein 1 (DNTTIP1) Protein (His)
Beta LifeScience
SKU/CAT #: BLC-10243P
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Description | Recombinant Human Deoxynucleotidyltransferase Terminal-Interacting Protein 1 (DNTTIP1) Protein (His) is produced by our E.coli expression system. This is a full length protein. |
Purity | Greater than 90% as determined by SDS-PAGE. |
Uniprotkb | Q9H147 |
Target Symbol | DNTTIP1 |
Synonyms | C20orf167; Deoxynucleotidyltransferase terminal interacting protein 1; Deoxynucleotidyltransferase terminal-interacting protein 1; dJ447F3.4; DNTTIP1; TdIF 1; TdIF1; TDIF1_HUMAN; TdT binding protein; TdT interacting factor 1; TdT-interacting factor 1; Terminal deoxynucleotidyltransferase interacting factor 1; Terminal deoxynucleotidyltransferase-interacting factor 1 |
Species | Homo sapiens (Human) |
Expression System | E.coli |
Tag | N-6His |
Target Protein Sequence | MGATGDAEQPRGPSGAERGGLELGDAGAAGQLVLTNPWNIMIKHRQVQRRGRRSQMTTSFTDPAISMDLLRAVLQPSINEEIQTVFNKYMKFFQKAALNVRDNVGEEVDAEQLIQEACRSCLEQAKLLFSDGEKVIPRLTHELPGIKRGRQAEEECAHRGSPLPKKRKGRPPGHILSSDRAAAGMVWKPKSCEPIRREGPKWDPARLNESTTFVLGSRANKALGMGGTRGRIYIKHPHLFKYAADPQDKHWLAEQHHMRATGGKMAYLLIEEDIRDLAASDDYRGCLDLKLEELKSFVLPSWMVEKMRKYMETLRTENEHRAVEAPPQT |
Expression Range | 1-329aa |
Protein Length | Full Length |
Mol. Weight | 41.0kDa |
Research Area | Epigenetics And Nuclear Signaling |
Form | Liquid or Lyophilized powder |
Buffer | Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Reconstitution | Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
Storage | 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
Target Details
Target Function | Increases DNTT terminal deoxynucleotidyltransferase activity (in vitro). Also acts as a transcriptional regulator, binding to the consensus sequence 5'-GNTGCATG-3' following an AT-tract. Associates with RAB20 promoter and positively regulates its transcription. Binds DNA and nucleosomes; may recruit HDAC1 complexes to nucleosomes or naked DNA. |
Subcellular Location | Nucleus. |
Database References |
Gene Functions References
- In luciferase assays, TdIF1 can up-regulate transcription activity of the promoters containing the TdIF1-binding sequences, and this effect is mainly through the palindrome 5'-TGCATG-3'. PMID: 25619743
- During cell division, DNTTIP1 is part of the mitotic deacetylase complex MIDAC, which also contains C14ORF43/MIDEAS and HDAC1/HDAC2. PMID: 21258344
- During cell division, DNTTIP1 forms a complex with C14ORF43/MIDEAS and HDAC1/HDAC2. PMID: 21258344
- The TdT binding, DNA binding and dimerization regions, and nuclear localization signal (NLS) in TdIF1, were identified. PMID: 17663723