Recombinant Human DDR1 Protein (aa 444-913, His & GST Tag)

Beta LifeScience SKU/CAT #: BLPSN-1567

Recombinant Human DDR1 Protein (aa 444-913, His & GST Tag)

Beta LifeScience SKU/CAT #: BLPSN-1567
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

Submit an inquiry today to inquire about all available size options and prices! Connect with us via the live chat in the bottom corner to receive immediate assistance.

Product Overview

Tag His&GST
Host Species Human
Accession Q08345
Synonym CAK, CD167, DDR, EDDR1, HGK2, MCK10, NEP, NTRK4, PTK3, PTK3A, RTK6, TRKE
Background Discoidin domain receptor family, member 1 (DDR1), also known as or CD167a (cluster of differentiation 167a), and Mammary carcinoma kinase 1 (MCK1), belongs to a subfamily of tyrosine kinase receptors with an extracellular domain homologous to Dictyostellium discoideum protein discoidin 1. Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. Expression of DDR1/MCK1/CD167 is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. DDR1/MCK1/CD167 plays an important role in regulating attachment to collagen, chemotaxis, proliferation, and MMP production in smooth muscle cells. DDR1 functions in a feedforward loop to increase p53 levels and at least some of its effectors. Inhibition of DDR1 function resulted in strikingly increased apoptosis of wild-type p53-containing cells in response to genotoxic stress through a caspase-dependent pathway.
Description A DNA sequence encoding the human DDR1 (Q08345-1) (Arg444-Val913) was fused with the N-terminal His-tagged GST tag at the N-terminus.
Source Baculovirus-Insect Cells
Predicted N Terminal Met
AA Sequence Arg444-Val913
Molecular Weight The recombinant human DDR1 /GST chimera consists of 707 a.a. and has a calculated molecular mass of 80 kDa. The recombinant protein migrates as an approximately 80 kDa band in SDS-PAGE under reducing conditions.
Purity >89% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity The specific activity was determined to be 2.75 nmol/min/mg using synthetic AXLtide peptide(CKKSRGDYMTMQIG) as substrate.
Formulation Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol, 3mM DTT.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function Tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing. Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing. Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Also plays a role in tumor cell invasion. Phosphorylates PTPN11.
Subcellular Location [Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Cell membrane; Single-pass type I membrane protein.; [Isoform 3]: Secreted.; [Isoform 4]: Cell membrane; Single-pass type I membrane protein.
Protein Families Protein kinase superfamily, Tyr protein kinase family, Insulin receptor subfamily
Database References
Tissue Specificity Detected in T-47D, MDA-MB-175 and HBL-100 breast carcinoma cells, A-431 epidermoid carcinoma cells, SW48 and SNU-C2B colon carcinoma cells and Hs 294T melanoma cells (at protein level). Expressed at low levels in most adult tissues and is highest in the b

Gene Functions References

  1. Data (including data from studies using knockout mice) suggest that DDR1 plays role in promoting mammary tumor growth; tumor-extrinsic DDR1 appears to be required for promotion of mammary tumors by interleukin-6. PMID: 29298894
  2. our study provides a novel regulatory pathway involving TM4SF1, DDR1, MMP2 and MMP9, which promotes the formation and function of invadopodia to support cell migration and invasion in pancreatic cancer. PMID: 28368050
  3. The three well-conserved seed matched sites for miR-199a/b-5p in the discoidin domain receptor 1 (DDR1) 3'-UTR were targeted, and miRNA binding to at least two sites was required for DDR1 inhibition. PMID: 29429150
  4. E2F1 knockdown decreased the expression of discoidin domain receptor 1 (DDR1) which plays a crucial role in many fundamental processes such as cell differentiation, adhesion, migration and invasion. PMID: 29039472
  5. Furthermore, the inhibition of DDR1 with 7rh showed striking efficacy in combination with chemotherapy in orthotopic xenografts and autochthonous pancreatic tumors where it significantly reduced DDR1 activation and downstream signaling, reduced primary tumor burden, and improved chemoresponse. PMID: 28864681
  6. These findings demonstrate a critical role of miR-199a-3p/DDR1 pathway in ovarian cancer development. PMID: 28743276
  7. findings demonstrate that, in human breast cancer cells, DDR1 regulates IR expression and ligand dependent biological actions. This novel functional crosstalk is likely clinically relevant PMID: 28591735
  8. These results support an activation mechanism of DDR1 whereby collagen induces lateral association of DDR1 dimers and phosphorylation between dimers. PMID: 28590245
  9. Data suggest that IGF-I/IGF-IR system triggers stimulatory actions through both GPER and DDR1 in aggressive tumors as mesothelioma and lung tumors. PMID: 27384677
  10. we further analyzed the CpG methylation levels at the DDR1 promoter in EOC cells and found that the CpG methylation levels of DDR1 promoter correlated negatively with the expression of DDR1 along the EMT spectrum. Therefore, EMT stratification could be a potential biomarker to predict patient response to DDR1-targeting drugs. PMID: 28887161
  11. This study suggested that DDR1 and DDR2 knockdown alters brain immunity and significantly reduces the level of triggering receptor expressed on myeloid cells (TREM)-2 and microglia. PMID: 28863860
  12. Isoform b of DDR1 is responsible for collagen I-induced up-regulation of N-cadherin and tyrosine 513 of DDR1b is necessary. PMID: 27605668
  13. Reduced DDR1 expression may be implicated in impaired melanocyte adhesion process involved in vitiligo pathogenesis PMID: 26091274
  14. data demonstrate that TGF-beta1 favors linear invadosome formation through the expressions of both the inducers, such as collagen and LOXL2, and the components such as DDR1 and MT1-MMP of linear invadosomes in cancer cells. Meanwhile, our data uncover a new TGF-beta1-dependent regulation of DDR1 expression. PMID: 27720259
  15. DDR1 overexpression promoted GC cell proliferation (p < 0.05), migration (p < 0.01), and invasion (p < 0.01), and accelerated the growth (p < 0.05) as well as the microvessel formation (p < 0.01) of transplantation tumor in nude mice. PMID: 27179963
  16. Our results suggest that DDR1 is both a prognostic marker for renal clear cell carcinoma and a potential functional target for therapy PMID: 27020590
  17. Study concludes that non-canonical DDR1 signaling enables breast cancer cells to exploit the ubiquitous interstitial matrix component collagen I to undergo metastatic reactivation in multiple target organs. PMID: 27368100
  18. Upregulation of DDR1 collagen receptor is associated with breast cancer. PMID: 26655502
  19. Knockdown DDR1 reversed the effects of Galpha13 knockdown on cell-cell adhesion and proteolytic invasion in three-dimensional collagen. PMID: 26589794
  20. Data suggest SCl2 (Streptococcus pyogenes) binds to DDR (DDR1, DDR2) ectodomain w/o stimulating receptor signaling; here, protein engineering was used to construct SCl-like proteins that inhibit collagen-DDR interactions and macrophage migration. PMID: 26702058
  21. DDR1 is a key modulator of RIT activity. PMID: 26719540
  22. DDR1 expression is a prognostic indicator in pancreatic ductal carcinoma. PMID: 26297342
  23. alpha5(IV), but not alpha1(IV), promotes lung cancer cell proliferation and tumor angiogenesis through non-integrin collagen receptor DDR1-mediated ERK activation. PMID: 25992553
  24. Hypoxia can increase DDR1 expression in pituitary adenoma cells, leading to improved MMP-2 and MMP-9 secretion, and promoting pituitary adenoma cell proliferation and invasion. PMID: 26286316
  25. suggest that DDR1 suppression may enhance adipose-derived stem cell chondrogenesis by enhancing the expression of chondrogenic genes and cartilaginous matrix deposition. PMID: 25673773
  26. MT1-MMP-discoidin domain receptor 1 axis regulates collagen-induced apoptosis in breast cancer cells PMID: 25774665
  27. Defective Ca(2+) binding in a conserved binding site causes incomplete N-glycan processing and endoplasmic reticulum trapping of DDR1 and DDR2 receptors. PMID: 25470979
  28. A DDR1(Low)/DDR2(High) protein profile is associated with TNBC and may identify invasive carcinomas with worse prognosis. PMID: 25667101
  29. We uncovered ten new mutations in TNK2 and DDR1 within serous and endometrioid ECs, thus providing novel insights into the mutation spectrum of each gene in EC. PMID: 25427824
  30. Silencing of DDR1 inhibited tumor cell growth and motility, and induced TGFBI expression, both in vitro and in vivo. PMID: 25369402
  31. found an inverse correlation between ZEB1 and DDR1 expression in various cancer cell lines and in human breast carcinoma tissues PMID: 25155634
  32. DDR1 depletion blocked cell invasion in a collagen gel PMID: 25422375
  33. The expression of the DDR1 protein significantly correlated with poor disease-free survival in patients with serous ovarian cancer. PMID: 21541037
  34. High DDR1 protein expression is associated with recurrence in ameloblastomas. PMID: 24723326
  35. In this review we highlight the mechanisms whereby DDRs(DDR1 and DDR2) regulate two important processes, namely inflammation and tissue fibrosis. PMID: 24361528
  36. Report aberrant methylation of DDR1 in men with nonobstructive azoospermia. PMID: 25064398
  37. Crystal structures of DDR1 reveal a DFG-out conformation (DFG, Asp-Phe-Gly) of the kinase domain that is stabilized by a salt bridge between the activation loop and alphaD helix. Differences to Abelson kinase are observed in the DDR1 P-loop's beta-hairpin. PMID: 24768818
  38. These findings indicate that the extracellular juxtamembrane region of DDR1 is exceptionally flexible and does not constrain the basal or ligand-activated state of the receptor. PMID: 24671415
  39. N-glycosylation at the highly conserved (211)NDS motif evolved to act as a negative repressor of DDR1 phosphorylation in the absence of ligand PMID: 24509848
  40. regulation of DDRs by glucose PMID: 24018687
  41. Upregulation of DDR1 induced by collagen I may contribute to the development and progression of NSCLC. PMID: 23761027
  42. The DDR1 extracellular domain plays a crucial role in receptor oligomerization which mediates high-affinity interactions with its ligand. PMID: 23810922
  43. We show that expression of the latent LMP1 in primary human germinal center B cells, the presumed progenitors of HRS cells, upregulates discoidin domain receptor 1 (DDR1), a receptor tyrosine kinase activated by collagen. PMID: 24136166
  44. these data suggest that NEP can augment taxane-induced apoptosis through inhibition of Akt/Bad signaling PMID: 22895534
  45. the expression of the novel collagen receptor discoidin domain receptor 1 (DDR1) by human MKs at both mRNA and protein levels and provide evidence of DDR1 involvement in the regulation of MK motility on type I collagen PMID: 23530036
  46. DDR1 expression was not predictive for patient survival in human breast cancer PMID: 23307244
  47. DD1 mRNA and protein levels were higher in patients with recurrent Hepatocellular carcinoma , suggesting this gene maybe involved in tumor invasion and metastasis. PMID: 22752569
  48. a role for the collagenase of membrane-type MMPs in regulation of DDR1 cleavage and activation at the cell-matrix interface. PMID: 23519472
  49. Discoidin domain receptors promote alpha1beta1- and alpha2beta1-integrin mediated cell adhesion to collagen by enhancing integrin activation. PMID: 23284937
  50. Discoidin domain receptors are unique receptor tyrosine kinases in collagen-mediated signaling [review] PMID: 23335507


Please fill out the Online Inquiry form located on the product page. Key product information has been pre-populated. You may also email your questions and inquiry requests to We will do our best to get back to you within 4 business hours.

Feel free to use the Chat function to initiate a live chat. Our customer representative can provide you with a quote immediately.

Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

Recently viewed