Recombinant Human DAO Protein (N-6His)

Name: Recombinant Human DAO Protein (N-6His)
Brand: Beta LifeScience
SKU: BL-1486NP
Availability: InStock

BL-1486NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

Submit an inquiry today to inquire about all available size options and prices! Connect with us via the live chat in the bottom corner to receive immediate assistance.

Product Overview

Description	Recombinant Human D-Amino-Acid Oxidase is produced by our E.coli expression system and the target gene encoding Met1-Leu347 is expressed with a 6His tag at the N-terminus.
Accession	P14920
Synonym	D-Amino-Acid Oxidase; DAAO; DAMOX; DAO
Gene Background	D-Amino-Acid Oxidase (DAO) belongs to the DAMOX/DASOX family. DAO is a peroxisomal enzyme which founctions as a homodimer to oxidizes D-amino acids to the corresponding imino acids, producing ammonia and hydrogen peroxide. D-amino-acid oxidase regulates the level of the neuromodulator D-serine in the brain, has a high activity towards D-DOPA and contributes to dopamine synthesis. D-amino-acid oxidase could act as a detoxifying agent which removes D-amino acids accumulated during aging. It also acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups.
Molecular Mass	41.64 KDa
Apmol Mass	40 KDa, reducing conditions
Formulation	Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 20% Glycerol, 1mM DTT, 2mM EDTA, pH 8.0.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution
Storage	Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
Shipping	The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids.
Subcellular Location	Peroxisome.
Protein Families	DAMOX/DASOX family
Database References	HGNC: 2671 OMIM: 124050 KEGG: hsa:1610 STRING: 9606.ENSP00000228476 UniGene: PMID: 28750137 The mutant G allele of the C2029G DAO polymorphism was significantly more frequent in patients with migraine (P=.044). The OR of migraine for carriers of the mutant G allele of DAO compared to wild-type homozygous women was 1.6 (95% CI, 1.1-2.1). PMID: 27130307 The study provides significant evidence for epistatic interactions among DAOA and DAO gene in the development of schizophrenia. These results provide additional evidence and indicate that the DAOA gene and DAOA-DAO gene-gene interactions might play a role for schizophrenia in a Taiwanese sample. PMID: 28285246 pLG72 interacts with neosynthesized d-amino acid oxidase (hDAAO), promoting its inactivation and degradation. In this work, we used low-resolution techniques to characterize the surface topology of the hDAAO-pLG72 complex. PMID: 27400736 lower levels predispose to disease exacerbation after fish intake in chronic urticaria patients PMID: 25982580 The DAO gene was a susceptibility gene for schizophrenia and the genomic region between intron 1 and intron 8 may harbor functional genetic variants, which may influence the mRNA expression of DAO and neurocognitive functions in schizophrenia. PMID: 26986737 In the brain, the concentration of D-serine stored in cells is defined by the activity of D-amino acid oxidase. PMID: 24256253 Accumulation of d-serine contributes to an ALS pathogenesis, and DAO might be a common therapeutic target for ALS. PMID: 24085347 Data suggest that neuronal DAAO is a long-lived protein: peroxisomal fraction of DAAO is degraded via lysosomal/endosomal pathway, whereas cytosolic fraction of DAAO is ubiquitinated and targeted to proteasome pathway. PMID: 24237903 Data suggest that acyclovir (ACV) can serve as an active site probe to study the structural basis of temperature-induced conformational changes of d-Amino acid oxidase (DAO). PMID: 23859606 The results suggest that DAO, which is involved in the N-methyl-d-aspartate receptor regulation, signaling and glutamate metabolism, is the master gene of the genetic associations and interactions underlying schizophrenia. PMID: 23555897 study suggests that NMDA receptor-mediated signalling genes, DAO, PPP3CC, DTNBP1 might be involved in schizophrenia pathogenic mechanisms related to gender PMID: 23497497 Data indicate that the protein aggregation due to the expression of the G331V d-amino acid oxidase (hDAAO) variant affects cell viability. PMID: 23219954 The DAO polymorphism rs10156191 which causes impaired metabolism of circulating histamine is associated with the clinical response in crossed-hypersensitivity to NSAIDs and could be used as a biomarker of response. PMID: 23152756 The distribution pattern of D-amino-acid oxidase gene and protein expression in the central nervous system is inversely correlated with D-serine content and distribution. (Review) PMID: 22865246 D-Amino acid oxidase-induced oxidative stress, 3-bromopyruvate and citrate inhibit angiogenesis, exhibiting potent anticancer effects. PMID: 22802136 A significant 3 way interaction between G72, DAAO and diagnosis is detected in the right middle temporal gyrus (after family-wise error correction), accounting for 8.5% of the individual variance in activation. PMID: 22239582 Genetic polymorphisms in DAO are associated with reduced prepulse inhibition and worse performance in working memory tasks and a personality pattern characterized by attenuated anxiety. PMID: 21471957 Data suggest that newly synthesized D-amino acid oxidase colocalizes and interacts with pLG72 which appears to be exposed on the external membrane of mitochondria. PMID: 21679769 The rs3918347 allele nucleotide polymorphism of DAAO is associated with schizophrenia in Chinese. PMID: 20855273 results suggest that genetic variation in DAAO has a significant impact on both regional activation and functional connectivity PMID: 21421061 D-amino acid oxidase activity is inhibited by an interaction with bassoon protein at the presynaptic active zone. PMID: 21700703 Data describe the the effects on human D-amino acid oxidase conformation and stability of the substrate D-serine, the FAD cofactor, and two inhibitors (benzoate and chlorpromazine. PMID: 20521334 This study supported the hypothesis that DAO plays a role in schizophrenia, possibly in a gender-dependent manner in Koreans. PMID: 20483168 The results of this study showed that DAO does not have an apparent degree of association with Japanese schizophrenia. PMID: 20178891 Familial amyotrophic lateral sclerosis is associated with a mutation in D-amino acid oxidase PMID: 20368421 increased hippocampal DAAO expression in schizophrenia; could be responsible for a decrease in local D-serine levels leading to a NMDA-receptor hypofunction that is hypothesized to play a major role in the pathophysiology of schizophrenia PMID: 19823762 results suggest that an increase in DAO expression in parts of the brain is involved in aberrant D-amino acid metabolism, may be a potential therapeutic target for schizophrenia PMID: 19685198 Creatinine (CTN) was found to inhibit D-amino acid oxidase in uremia PMID: 12053066 The association of both DAAO and a new gene G72 from 13q34 with schizophrenia together with activation of DAAO activity by a G72 protein product points to the involvement of this N-methyl-d-aspartate receptor regulation pathway in schizophrenia. PMID: 12364586 DAAO was associated with schizophrenia, but not with bipolar disorder PMID: 14966479 This study indicates that the DAAO gene may play a significant role in the etiology of schizophrenia in the Han Chinese. PMID: 15464270 the human enzyme is a stable homodimer even in the apoprotein form and weakly binds the cofactor in the free form. PMID: 16616139 We found elevated cerebellar DAAO activities in post-mortem brain samples from schizophrenic versus normal individuals. PMID: 16828464 Crystal structure of human D-amino acid oxidase. PMID: 17088322 The genes D-Amino-Acid Oxidase is regulate the glutamate neurotransmission in schizophrenia. PMID: 17250995 might exert gene-specific modulating effects on schizophrenia endophenotypes at the population level PMID: 17336946 evidence for epistatic interaction between the associated SNPs at DAOActivator and D-amino acid oxidase genes in contributing to schizophrenia risk PMID: 17492767 Data suggest that the D-amino acid oxidase activator (DAOA/G30) locus, but not D-amino acid oxidase, may play a role in the pathophysiology of schizophrenia. PMID: 17627036 significant associations between the rs3918346 and rs3825251 SNPs of the DAO gene and boys with autism spectrum disorders PMID: 17629951 Polymorphisms are not associated with homicidal behavior in korean schizophrenics. PMID: 17728673 These data suggest a potential role for DAO in susceptibility to depressive symptoms in schizophrenia, but a more general role for DAO in affective disorders cannot be excluded. PMID: 17890006 Some support for the individual involvement of DAO and G72(DAOA)/G30 in the etiology of bipolar disorder. PMID: 18165970 a decrease in the synaptic concentration of d-serine as the result of an anomalous increase in hDAAO activity related to hypoexpression of pLG72 may represent a molecular mechanism by which hDAAO and pLG72 are involved in schizophrenia susceptibility PMID: 18544534 DAO activity and expression are increased in schizophrenia, but are not related to DAO or G72/G30 genotype PMID: 18560437 Flavin-Adenine Dinucleotide binding only slightly increases the stability of human DAAO to denaturation by urea or temperature. PMID: 19309736 present Scandinavian results do not verify previous associations between the analyzed DTNBP1, NRG1, DAO, DAOA, and GRM3 gene polymorphisms and schizophrenia PMID: 19439994 The present association of dysbindin SNPs with negative symptoms and DAO SNPs with anxiety and depression is a replication of earlier findings and strengthens the hypothesis of a genetic association in schizophrenia. PMID: 19729970

FAQs

Please fill out the Online Inquiry form located on the product page. Key product information has been pre-populated. You may also email your questions and inquiry requests to sales1@betalifesci.com. We will do our best to get back to you within 4 business hours.

Feel free to use the Chat function to initiate a live chat. Our customer representative can provide you with a quote immediately.

Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

📧 inquiry@betalifesci.com 📞 +1(201) 888-7983

Contact Us Now for Inquiries, Orders, and Questions

Recombinant Human DAO Protein (N-6His)

Recombinant Human DAO Protein (N-6His)

Product Overview

Target Details

FAQs