Recombinant Human Cystatin F / CMAP Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1536
Recombinant Human Cystatin F / CMAP Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1536
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Human |
Accession | NP_003641.3 |
Synonym | CMAP |
Background | The cystatin superfamily members are important natural cysteine protease inhibitors present in a wide variety of organisms and are divided into three classes. Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a type 2 cystatin and its expression is limited to hematopoietic cells, with the highest expression levels being observed in monocytes, dendritic cells, and certain types of T-cells. Furthermore, cystatin F mRNA becomes up-regulated during dendritic cell maturation, and thus suggests a specific role of cystatin F in immune regulation. Cystatin F is produced as a dimer, an inactive cathepsin inhibitor which is activated by chemical reduction. In addition, Cystatin F and its homologues have been observed expressing in various human cancer cell lines established from malignant tumors, and thus indicates a new diagnosis and prevention approach of certain human carcinomas metastasis. |
Description | A DNA sequence encoding the human Cystatin F precursor (NP_003641.3) (Met 1-His 145) was expressed,with a His tag at the C-terminus. |
Source | HEK293 |
Predicted N Terminal | Gly 20 |
AA Sequence | Met 1-His 145 |
Molecular Weight | Cystatin F exists as a disulfide-linked homodimer. The human cystatin F monomer consists of 137 a.a. and and has a predicted molecular mass of 16.1 kDa. As a result of glycosylation, the recombinant Cystatin F migrates as an approximately 44 kDa band in SDS-PAGE under non-reduced conditions corresponding to the dimmer. |
Purity | >92% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC (R&D Systems, Catalog # ES008). The IC50 value is <6 nM. |
Formulation | Lyophilized from sterile PBS, pH 7.4. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |