Recombinant Human Cyclin-Dependent Kinase Inhibitor 1 (CDKN1A) Protein (GST)

Name: Recombinant Human Cyclin-Dependent Kinase Inhibitor 1 (CDKN1A) Protein (GST)
Brand: Beta LifeScience
SKU: BLC-10081P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Human Cyclin-Dependent Kinase Inhibitor 1 (CDKN1A) Protein (GST) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P38936
Target Symbol	CDKN1A
Synonyms	CAP20; CDK-interacting protein 1; CDKI; CDKN1; Cdkn1a; CDN1A_HUMAN; CIP1; Cyclin Dependent Kinase Inhibitor 1A; Cyclin-dependent kinase inhibitor 1; Cyclin-dependent kinase inhibitor 1A (P21); Cyclin-dependent kinase inhibitor 1A (p21, Cip1); DNA Synthesis Inhibitor; MDA-6; MDA6; Melanoma differentiation-associated protein 6; Melanoma differentiation-associated protein; p21; P21 protein; p21CIP1; p21Cip1/Waf1; p21WAF; PIC1; SDI1; SLC12A9; WAF1; Wild type p53 activated fragment 1 (WAF1); Wild type p53 activated fragment 1; Wildtype p53-activated fragment 1
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-GST
Target Protein Sequence	SEPAGDVRQNPCGSKACRRLFGPVDSEQLSRDCDALMAGCIQEARERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQGTAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFSKRKP
Expression Range	2-164aa
Protein Length	Full Length of Mature Protein
Mol. Weight	45.0kDa
Research Area	Cell Cycle
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	May be involved in p53/TP53 mediated inhibition of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. Inhibits DNA synthesis by DNA polymerase delta by competing with POLD3 for PCNA binding. Plays an important role in controlling cell cycle progression and DNA damage-induced G2 arrest.
Subcellular Location	Cytoplasm. Nucleus.
Protein Families	CDI family
Database References	HGNC: 1784 OMIM: 116899 KEGG: hsa:1026 STRING: 9606.ENSP00000244741 UniGene: PMID: 29118075 No significant differences were found in the frequency of alleles of CDKN1A gene in the group of healthy children and children with Down syndrome and dental caries. PMID: 29578436 In vitro studies in human lung fibroblasts revealed increased levels of p21 (p = 0.0032) and pAkt (p = 0.12) following treatment with serotonin. PMID: 29386571 Data suggest that CRNDE may function as an oncogene by modulating p21, finally contributing to the radioresistant phenotype formation of LAD cells. PMID: 28550688 Cyclin dependent kinase inhibitor 1A (p21) expressions is upregulated in non-cycling HCT116 p53(+/+) cells and HIV-1 reverse transcription is subsequently inhibited. The restrictions of HIV by p21 is associated with the suppression of ribonucleotide reductase R2 subunit expression and phosphorylation of SAMHD1 protein. siRNA knockdown of p21 increased HIV-2 infection in human monocyte derived macrophages. PMID: 29587790 Data indicate how cyclin dependent kinase inhibitor 1A (p21) regulates the proliferation-quiescence decision to maintain genomic stability. PMID: 28317845 The first evidence for non-repair functions of MGMT in cell cycle and highlight the involvement of PCNA in MGMT downregulation, with p21 attenuating the process. PMID: 29510343 Scriptaid was also able to dose dependently and significantly induce MM cell cycle arrest at the G2/M phase. PMID: 29305109 High CDKN1A expression is associated with migration, invasion, and progression of bladder cancer. PMID: 29602637 CDKN1A role in DANCR-mediated tumor cell growth. PMID: 29180471 CDKN1A expression was seen only in five cases and that too focally in the cytoplasm, thereby warranting removal of analysis of CDKN1A positivity from the study PMID: 29893337 Results show that knockdown of GABPB1 in clear cell renal cell carcinoma cell lines significantly decreased the ability to form colonies by inducing the expression of p21Waf/Cip1. PMID: 29845229 Authors demonstrate that HMGB2 transcription is repressed by p21 during radiation-induced senescence through the ATM-p53-p21 DNA damage signaling cascade. The loss of p21 abolished the downregulation of HMGB2 caused by ionizing radiation, and the conditional induction of p21 was sufficient to repress the transcription of HMGB2. PMID: 29487276 Low CDKN1A expression is associated with cervical cancer. PMID: 30098344 p21 was involved in glioma cell proliferation after SNHG6 was downregulated. PMID: 29579705 the CDK inhibitor p21 begins rising in G2 in mother cells whose daughters exit mitosis into the pre-Restriction Point, CDK2(low) state. Furthermore, degradation of p21 coincides with escape from the CDK2(low) state and passage through the Restriction Point. PMID: 30111539 SAC decreased the levels of 5-methylcytosine, DNMT activity, messenger RNA (mRNA) and protein levels of DNMT1. Additionally, SAC treatment resulted in re-expression of the mRNA and proteins of silenced tumor suppressor gene CDKN1A accompany with reduced cell division control 2 expression. PMID: 29759079 Down-regulation of HOTAIR elicits an inhibitory effect on proliferation, invasion, and migration, while promoting the apoptosis of colorectal cancer cells through the up-regulation of p21. PMID: 29808247 Glutaredoxin-1 silencing induces cell senescence via p53/p21/p16 signaling axis. PMID: 29356545 p16, p21, and p53 proteins play an important role in the deregulation of the cell cycle and participate in the development of pancreatic intraepithelial neoplasia. PMID: 29388054 Here, the authors demonstrated that CUL4B forms an E3 ligase with RBX1 (RING-box 1), DDB1 (DNA damage binding protein 1), and DCAF11 (DDB1 and CUL4 associated factor 11) that promotes the ubiquitination of p21(Cip1) and regulates cell cycle progression in human osteosarcoma cells. PMID: 28446751 Low CIP1 expression is associated with gastric cancer. PMID: 30031062 PAK1 is upregulated in cutaneous T cell lymphoma. PAK1 silencing induced apoptosis and inhibited cell growth by stimulating the expression of PUMA and p21. PMID: 29307600 These findings reveal an important mechanism by which p21 can be stabilized by direct deubiquitylation, and they pinpoint a crucial role of the USP11-p21 axis in regulating cell-cycle progression and DNA damage responses. PMID: 29666278 Polymorphisms in TP53 and P21 proteins are associated with an increased risk of stomach cancer. PMID: 29124536 A chimeric cDNA construct of human p53 was made where the 1-260 bp N-terminus was replaced with buffalo p53 counterpart and expressed in H1299 cell line. The tetramerization ability of the chimeric p53 protein was comparable to that of h-p53. Properties of b-p53 like stronger p21 transactivation and super sensitivity to Mdm2 mediated degradation were lacking in the chimeric protein. PMID: 29147811 The associated effects appear to be mediated by inhibition of IGFBP-2 expression and stimulation of p21 expression. This suggests that simulated microgravity might represent a promising method to discover new targets for glioma therapeutic strategy. PMID: 28707224 variants of EGFR and SYNE2 play an important role in p21 regulation and are associated with the clinical outcome of HBV-related hepatocellular carcinoma in a TP53-indenpdent manner PMID: 27502069 GRh2 dose-dependently inhibited prostate cancer cell proliferation, but did not alter cell apoptosis, seemingly through downregulation of miR-4295, which inhibits protein translation of CDKN1A. PMID: 29457293 These results demonstrate that miR-95-3p is a potential new marker for Hepatocellular carcinoma and regulates hepatocarcinogenesis by directly targeting CDKN1A/p21 expression. PMID: 27698442 rescue experiments indicated that SNHG20 functioned as an oncogene partly via repressing p21 in non-small cell lung cancer (NSCLC) cells. Taken together, our findings demonstrate that SNHG20 is a new candidate for use in NSCLC diagnosis, prognosis and therapy. PMID: 28981099 Expression of CIP/KIP proteins was found abundantly within the proliferative hair matrix, concomitant with a role in cell cycle checkpoint control. p21(CIP1), p27(KIP1) and cyclin E persisted within post-mitotic keratinocytes of the pre-cortex, whereas p57(KIP2) protein decreased but became nuclear. PMID: 28413121 AIbZIP induced by the androgen receptor axis plays a crucial role in the p21 dependent proliferation of androgen-sensitive prostate cancer cells. PMID: 27853318 both the p53-Puma/Noxa/Bax pathway and the cell cycle arrest-associated p53-p21 pathway were involved in the AZT-induced cell cycle arrest (p53-p21) and DNA double-strand breaks (gamma-H2AX), while euploid cells were more sensitive to AZT-induced apoptosis (p53-Puma/Bax/Noxa). PMID: 28627647 The transcriptional regulation of the p21 promoter by iron chelators was demonstrated to be dependent on the chelator and cell-type examined.A 50-bp region between -104 and -56-bp was required for Dp44mT-induced activation in SK-MEL-28 cells. It had several Sp1-binding sites. The Sp1-3-binding site played a significant role in Dp44mT-induced p21 activation. Dp44mT increased the interactions of Sp1 and ERalpha and c-jun. PMID: 29032246 serpinB2 bound to and stabilized p21 to mediate senescence in a proteasome-independent manner, indicating that serpinB2 has a direct role in senescence. Thus, this study reveals a unique mechanism by which serpinB2 maintains senescence through stabilization of p21 protein levels. PMID: 28794016 cMyc promotes rhabdomyosarcoma development by inhibiting apoptosis through repression of p21 transcription. PMID: 28765944 The results suggest that p53 simultaneously controls multiple pathways to induce cellular senescence through p21 and Akt. PMID: 28691365 Data show that cortactin-mediated p21Cip1 nuclear export and degradation facilitating MCP1-induced human aortic smooth muscle cell (HASMC) proliferation. PMID: 27363897 Low P21 expression is associated with clear cell and endometrioid carcinoma of the ovary and the endometrium. PMID: 29451900 PAK4 downregulated the level of p21 and enhanced the activity of Akt as well. And we conclude that PAK4 acts as a regulator of cell cycle progression of vascular smooth muscle cells by mediating Akt signaling and controlling p21 levels, which further modulate intimal hyperplasia and vascular smooth muscle cells proliferation PMID: 28706947 CBX3 promotes tumor proliferation by regulating G1/S phase via p21 downregulation and associates with poor prognosis in tongue squamous cell carcinoma PMID: 29462646 The results presented herein highlight the importance of p21Cip1 and p27Kip1 in the cell cycle control and drug resistance of glioma stem cells, providing new insights into the field of glioma biology. PMID: 28582703 LncRNA-ANCR inhibited the cell proliferation, migration, and invasion of osteosarcoma cells, possibly through interacting with EZH2 and regulating the expression of p21 and p27. PMID: 28679390 p21 is a bona fide ubiquitylation substrate for CHIP.p21 role in lung cancer radioresistance. PMID: 28232384 PVT1 played a pivotal part on the regulation of p21 expression in breast cancer cell lines. PMID: 28534994 Thus, the present study indicated that parkin knockout inhibits neural stem cell differentiation by JNK-dependent proteasomal degradation of p21. PMID: 28656059 Our findings show that the overexpression of p21;{Waf1/Cip1}, down-expression of p57;{Kip2} and gene promoter methylation of p57;Kip2 could be considered as promising diagnostic markers for breast cancer PMID: 28106536 PKCzeta was specifically involved in ACOT7 depletion-mediated cell cycle arrest as an upstream molecule of the p53-p21 signaling pathway in MCF7 human breast carcinoma and A549 human lung carcinoma cells. PMID: 28518146 HNF1A-AS1 promoted HCC cell proliferation by repressing the NKD1 and p21 expression. PMID: 28292020

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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