Recombinant Human CTLA-4 Protein (His & Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1477
Recombinant Human CTLA-4 Protein (His & Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1477
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His&Fc |
Host Species | Human |
Accession | NP_005205.2 |
Synonym | GSE, CD152, IDDM12, CELIAC3, CTLA-4. |
Background | CTLA-4 is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. |
Description | A DNA sequence encoding the human CTLA4 (NP_005205.2) extracellular domain (Met 1-Phe 162) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus. |
Source | HEK293 |
Predicted N Terminal | Ala 37 |
AA Sequence | Met 1-Phe 162 |
Molecular Weight | The recombinant human CTLA4/Fc is a disulfide-linked homodimer. The reduced monomer consists of 374 amino acids and has a predicted molecular mass of 41.6 kDa. As a result of glycosylation, the apparent molecular mass of rh CTLA4/Fc monomer is approximately 50-55 kDa in SDS-PAGE under reducing conditions. |
Purity | Greater than 92% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Please contact us for detailed information |
Formulation | Lyophilized from sterile PBS, pH 7.4 |
Stability | Recombinnat Proteins are stable for up to 1 year from date of receipt at -70°C |
Usage | For Research Use Only |
Storage | Store recombinant protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |