Recombinant Human CTHRC1 Protein (C-6His)

Name: Recombinant Human CTHRC1 Protein (C-6His)
Brand: Beta LifeScience
SKU: BL-0777NP
Availability: InStock

BL-0777NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Collagen Triple Helix Repeat-containing Protein 1 is produced by our Mammalian expression system and the target gene encoding Ser31-Lys243 is expressed with a 6His tag at the C-terminus.
Accession	Q96CG8
Synonym	Collagen triple helix repeat-containing protein 1; Protein NMTC1; CTHRC1
Gene Background	Collagen triple helix repeat-containing protein 1 is a protein that in humans is encoded by the CTHRC1 gene. It acts as a negative regulator of collagen matrix deposition. It may cause the disease of Barrett esophagus . Patients with Barrett esophagus have an increased risk of esophageal adenocarcinoma. The main cause of Barrett esophagus is gastroesophageal reflux. The retrograde movement of acid and bile salts from the stomach into the esophagus causes prolonged injury to the esophageal epithelium and induces chronic esophagitis, which in turn is believed to trigger the pathologic changes.
Molecular Mass	24.1 KDa
Apmol Mass	25-30 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	May act as a negative regulator of collagen matrix deposition.
Subcellular Location	Secreted, extracellular space, extracellular matrix.
Database References	HGNC: 18831 OMIM: 610635 KEGG: hsa:115908 STRING: 9606.ENSP00000330523 UniGene: PMID: 29631554 High Collagen Triple Helix Repeat Containing 1 Expression is associated with Osteosarcoma. PMID: 29970438 E6/E7-p53-POU2F1-CTHRC1 axis promotes cervical cancer cell invasion and metastasis PMID: 28303973 IL-1beta and CTHRC1 are upregulated in patients with Osteoarthritis. PMID: 29393342 CTHRC1 interacts with integrin beta3 and accelerates the FAK phosphorylation to promote ovarian cancer cell adhesion, migration and invasion in vitro and in vivo. PMID: 29021002 CTHRC1 plays a pivotal role in a great many fields, including increases bone mass, prevents myelination, reverses collagen synthesis in keloid fibroblasts, and increases fibroblast-like synoviocytes migration speed and abundant production of arthritic pannus in rheumatoid arthritis PMID: 28901303 CTHRC1, negatively regulated by miR-30c, promoted cell proliferation, invasion and migration and suppressed cell apoptosis in breast cancer, which might be by activating GSK-3beta/beta-catenin signaling and inhibiting Bax/Caspase-9/Caspase-3 signaling respectively. PMID: 28697793 Our data suggest that CTHRC1 may act as an oncogenic driver in progression and metastasis of ESCC, and may serve as a potential biomarker for prognosis and personalized therapy. PMID: 28645305 The negative and sensitivity-predictive values of CTHRC1 staining were excellent for both lymph node and peritoneal metastases. PMID: 27870703 High CTHRC1 expression is associated with metastatic melanomas. PMID: 26918341 CTHRC1 was established as a novel marker of activated synoviocytes in murine experimental arthritis and rheumatoid arthritis PMID: 27430622 ANOS1 and its co-expression partner, CTHRC1, promote the development and metastasis of colorectal cancer. PMID: 28854193 Expression of CTHRC1 was significantly higher in Wilms' tumor compared to the expression in the adjacent non-cancerous tissues. High tumor expression of CTHRC1 was associated with tumor size, clinical stage, histopathological type, and vascular invasion/metastasis. Patients with high CTHRC1 expression also exhibited a shorter survival. PMID: 27230801 CTHRC1 expression is significantly upregulated in human masticatory mucosa during wound healing. PMID: 28005267 CTHRC1 downregulation inhibited proliferation. PMID: 28281968 The knockdown of CTHRC1 exerts inhibitory effects on the proliferation and migration ability of glioblastoma cells. PMID: 28277194 CTHRC1 may play a role in the progression of ovarian cancer. PMID: 27779718 this study shows that the serum CTHRC1 level was significantly higher in the influenza A virus infection patients than in the healthy individuals; the influenza A virus non-structural protein NS1 upregulates the expression of CTHRC1 protein PMID: 27718266 Gene expression levels of three randomly selected DEGs, VCAN, COL5A1 and KCNJ16, were examined using RT-PCR in 10 ATC samples.. angiogenesis was activated by the high expression of CTHRC1, VCAN and POSTN, providing necessary nutrition for tumor cells PMID: 27599582 increased CTHRC1 expression is associated with advanced TNM stage, increased LN metastasis and tumor size, and decreased OS and DFS, indicating that CTHRC1 may be a biomarker for prognosis of cancer patients PMID: 27323076 High CTHRC1 expression is associated with Osteosarcoma. PMID: 27043295 Authors showed that ectopic transfection of CTHRC1 in EOC cells up-regulated the expression of EMT markers such as N-cadherin and vimentin, and EMT-associated transcriptional factor Snail. PMID: 26452130 CTHRC1 is secreted both by colorectal epithelia cells and stromal fibroblasts. CTHRC1 overexpression promotes colorectal cancer cell migration, invasion and proliferation in vitro. PMID: 26722469 determined the mRNA and protein expression of CTHRC1 in oral squamous cell carcinoma and evaluated the clinical and prognostic impact of CTHRC1 overexpression PMID: 26664254 CTHRC1 has a novel role in viral infection. PMID: 26180054 HBV facilitates HCC development through activating the oncoprotein CTHRC1. PMID: 25263696 CTHRC1 was overexpressed in human non-small cell lung cancer tissues and non-small cell lung cancer cell lines at the protein and mRNA level. PMID: 25238260 let-7b may directly target Cthrc1 and function as a tumor suppressor gene in gastric cancer. PMID: 25510669 CTHRC1 acts as a prognostic factor and promotes invasiveness of gastrointestinal stromal tumors by activating Wnt/PCP-Rho signaling. PMID: 24726140 Cthrc1 overexpression was associated with non-small cell lung cancers. PMID: 25139095 Cthrc1 is a pituitary hormone with significantly elevated levels in subjects carrying variant alleles of the melanocortin-1 receptor as wells as in patients with inflammatory conditions. PMID: 24945147 CTHRC1 has the potential to be a new biomarker for the aggressive hepatocellular carcinoma PMID: 24841500 CTHRC1 expression is elevated in human colon cancer cell lines and clinical specimens, and promotes cancer cell invasivity through ERK-dependent induction of MMP9 expression. PMID: 24504172 Studied CTHRC1 expression in human breast cancer tissue. A significant increase of CTHRC1 mRNA expression was seen in breast cancer tissue compared to the normal tissue from the same patients using RT-PCR and real-time PCR. PMID: 23658133 Overexpression of CTHRC1 in hepatocellular carcinoma promotes tumor invasion and predicts poor prognosis. PMID: 23922981 Rs35500845 in the CTHRC1 gene was associated with Paget's disease of bone in the French-Canadian population. PMID: 24370779 high Cthrc1 expression was an independent prognostic factor for both overall survival and disease-free survival of patients with gastric carcinoma PMID: 24746208 Data suggest that in oral squamous cell carcinoma (OSCC), dysregulation of canonical Wnt signaling and DPAGT1-dependent N-glycosylation induces CTHRC1, thereby driving OSCC cell migration and tumor spread. PMID: 23703614 this is the first demonstration of Cthrc1 as a marker of the severity of the disease progression in the dystrophic muscles. PMID: 23274062 the results of our study suggest that increased expression of CTHRC1 is associated with peritoneal carcinomatosis in Colorectal cancer patients PMID: 23359115 CTHRC1 has a role in pancreatic cancer progression and metastasis by regulating migration and adhesion activities of cancer cells. PMID: 23222813 Data indicate that the upregulated expression of collagen triple helix repeat containing 1 (CTHRC1) in gastric carcinogenesis contributes to tumor cell invasion and metastasis. PMID: 22590977 Three major genes, MSR1, ASCC1, and CTHRC1 were associated with Barrett esophagus/esophageal adenocarcinoma PMID: 21791690 CTHRC1 is transiently expressed in the arterial wall in response to injury where it may contribute to vascular remodeling by limiting collagen matrix deposition and promoting cell migration. PMID: 15618538 Aberrant expression of CTHRC1 is widely present in human solid cancers and seems to be associated with cancer tissue invasion and metastasis. PMID: 16778098 Intracellular localization of Cthrc1 characterizes differentiated smooth muscle. PMID: 18467647

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.