Recombinant Human Citrate Synthase Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1274
Recombinant Human Citrate Synthase Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1274
Catalog No.: BLPSN-1274
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | O75390 |
| Background | Chondroitin sulphate (CS) glycosaminoglycan chains on cell and extracellular matrix proteoglycans (PGs) can no longer be regarded as merely hydrodynamic space fillers. Overwhelming evidence over recent years indicates that sulphation motif sequences within the CS chain structure are a source of significant biological information to cells and their surrounding environment. CS sulphation motifs have been shown to interact with a wide variety of bioactive molecules, e.g. cytokines, growth factors, chemokines, morphogenetic proteins, enzymes and enzyme inhibitors, as well as structural components within the extracellular milieu. They are therefore capable of modulating a panoply of signalling pathways, thus controlling diverse cellular behaviours including proliferation, differentiation, migration and matrix synthesis. Chondroitin sulfate (CS) is a sulfated glycosaminoglycan composed of a long chain of repeating disaccharide units that are attached to core proteins, resulting in CS proteoglycans (CSPGs). In the mature brain, CS is concentrated in perineuronal nets (PNNs), which are extracellular structures that surround synapses and regulate synaptic plasticity. In addition, CS is rapidly synthesized after CNS injury to create a physical and chemical barrier that inhibits axon growth. |
| Description | A DNA sequence encoding the human CS (O75390) (Met1-Gly466) was expressed with a C-terminal His tag. |
| Source | Baculovirus-Insect Cells |
| Predicted N Terminal | Ala 28 |
| AA Sequence | Met1-Gly466 |
| Molecular Weight | The secreted recombinant human CS consists of 449 a.a. and predicts a molecular mass of 50.4 KDa. The apparent molecular mass of the protein is approximately 46 KDa in SDS-PAGE under reducing conditions. |
| Purity | >90% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.0.. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
