Recombinant Human chymase 1 / MCT1 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-1266

Recombinant Human chymase 1 / MCT1 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-1266
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Product Overview

Tag His
Host Species Human
Accession P23946
Synonym chymase, CYH, MCT1
Background The STR polymorphism in the CMA1 gene is associated with asthma and that this association is even stronger with atopic asthma. CMA1 and IL-4 in atopic asthma and for IL-4 in atopy in general. The local angiotensin II system (LAS) has numerous functions, including the regulation of growth and differentiation in the gastrointestinal tract. Angiotensin II (AngII) may be generated by angiotensin-I-converting enzyme (ACE) or mast cell chymase (CMA1) and plays an important role in inflammatory processes, although opinions differ as to which AngII-generating enzyme is primarily associated with AngII-mediated effects. ACE in the gastric mucosa and the microvasculature of granulation tissue may represent a novel therapeutic target for the promotion of healing processes in gastritis and ulceration using ACE inhibitors or AT1R antagonists. The gene for mast cell chymase (CMA1) is an ideal candidate for investigating genetic predisposition to atopic asthma, as it is an important mediator of inflammation and remodeling in the asthmatic lung. (CMA1) is important for the generation of angiotensin II and therefore might be associated with the pathogenesis of hypertension.
Description A DNA sequence encoding the human CMA1 (P23946) (Met 1-Asn 247) was fused with a His tag at the C-terminus.
Source Baculovirus-Insect Cells
Predicted N Terminal Gly 20
AA Sequence Met 1-Asn 247
Molecular Weight The secreted recombinant human CMA1 consists of 238 a.a. and predicts a molecular mass of 26.6 kDa. It migrates as an approximately 33 kDa band in SDS-PAGE under reducing conditions.
Purity >92% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Please contact us for detailed information
Formulation Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.
Subcellular Location Secreted. Cytoplasmic granule. Note=Mast cell granules.
Protein Families Peptidase S1 family, Granzyme subfamily
Database References
Tissue Specificity Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions.

Gene Functions References

  1. These findings reveal the critical role of hypoxia in producing local angiotensin II by a lactate-chymase-dependent mechanism and highlight the importance of local angiotensin II in regulating radioresistance of hypoxic tumor cells. PMID: 28205588
  2. Cleavage of the alarmins by Human mast cell chymase and human neutrophil cathepsin G suggests a function in regulating excessive inflammation. PMID: 28053237
  3. activation of endothelial CLP/chymase may directly relate to the increased inflammatory phenotypic changes in the vascular system in women with preeclampsia PMID: 19494363
  4. Placenta-derived chymotrypsin-like protease contributes to the altered endothelial barrier function in preeclampsia. PMID: 19126871
  5. promoter single nucleotide polymorphism is not associated with Dengue Hemorrhagic Fever and Dengue Shock Syndrome in Vietnam and Philippines PMID: 25797204
  6. Our data demonstrated that mast cell chymase plays an important role in keloid formation through TGF-beta1/Smad signaling pathway. PMID: 25120737
  7. These data suggest a possible contribution of human chymase activation to LPS-induced mortality PMID: 24516344
  8. we present data indicating that MC tryptase and chymase contribute to the development of OSF and malignant transformation of the overlying epithelium. PMID: 24874976
  9. The polymorphisms and haplotypes of the CMA1 locus are associated with cardiac hypertrophy in male patients with symptomatic aortic stenosis. PMID: 24823657
  10. CMA1 gene single nucleotide polymorphisms is associated with essential hypertension. PMID: 25119908
  11. Significant association between the AG genotype of CMA1 A polymorphism and Angina Pectoris and Ventricular Extrasystoles was observed. PMID: 25102745
  12. The accumulated data in this review suggest that mast cells, their tryptases, and their chymases play important roles in tissue repair. PMID: 24507159
  13. demonstrate the generation of Pso p27 from SCCA1 with extracts from psoriatic scale and even more remarkably, the generation of Pso p27 from SCCA1 in the presence of mast cell associated chymase PMID: 24560885
  14. The present results indicated PRCP rs7104980 can be considered as a marker for EH and Hap3 GAGCACTAACA (PRCP) and Hap16 TTTA (CMA1) might be associated with essential hypertension in Chinese Han population. PMID: 22679278
  15. Purified chymase mixed with fragmented heparin derived from heparanase-expressing cells show greater release from collagen gels than the enzyme alone or mixed with macromolecular heparin derived from mock cells. PMID: 24344642
  16. Data indicate that the best Fynomer (the binding proteins derived from the Fyn SH3 domain) was found to bind chymase with a KD of 0.9 nM and koff of 6.6x10 (-4) s (-1) , and to selectively inhibit chymase activity with an IC 50 value of 2 nM. PMID: 22653218
  17. Both IgE and chymase associate with diabetes status. PMID: 22194960
  18. As mast cells are an important source of VEGF, tryptase, and chymase, these findings suggest that mast cell activation and mast cell-derived mediators participate in the development of Dengue hemorrhagic fever. PMID: 22363824
  19. Chymase rs1800875 polymorphism is associated with the progression of immunoglobulin A (IgA) nephropathy in Korean patients. PMID: 21150220
  20. a dominant role of cardiac chymase in the formation of Ang II from Ang-(1-12) PMID: 22180785
  21. Two different populations of mast cells were found in melanocytic skin; one expressing both chymase and tryptase and the other with tryptase only. PMID: 22102069
  22. mast cell-derived protein is present in uterine cervical carcinoma PMID: 21274549
  23. Polymorphism rs1800875 of CMA1 may be associated with serum IgE level in coronary heart disease subjects, but not with chymase level in normal coronary intimae PMID: 21796807
  24. human chymase contributes to blood glucose levels and mortality during the progression of diabetes PMID: 21786536
  25. short tandem repeat genetic polymorphism is associated with bronchial asthma in a Swiss cohort study PMID: 20736038
  26. Elevated maternal chymase activity and enhanced protease immunostaining in the maternal vessel endothelium may constitute the exacerbated inflammatory state and account for the increased vascular Ang II sensitivity in preeclampsia. PMID: 20670150
  27. Positions 143 (Arg) and 192 (Lys) in human mast cell chymase contribute to the strong preference for negatively charged amino acids at substrate position P2'. PMID: 20423454
  28. The levels of tryptase and chymase expression are greatly increased in human lung tissue of anaphylactic shock. PMID: 19697770
  29. The S1 primary specificity pocket defines substrate specificity of chymases. PMID: 11852067
  30. The mast cell chymase A3255 allele was shown to have an effect on HDL cholesterol metabolism. PMID: 12047032
  31. The local release of mast cell chymase has potentially profound effects on airway smooth muscle cell function by disruption of the cell-associated matrix and inhibition of epidermal growth factor-induced smooth muscle cell proliferation. PMID: 12097409
  32. Results suggest the additive effect of angiotensin I-converting enzyme (ACE) and heart chymase (CMA) gene polymorphisms on the increase in left ventricular mass in NIDDM patients. PMID: 12165749
  33. Chymase may play a role in heart remodeling by increasing Ang II formation and activating MMP-9, and the regulation of collagen I gene expression. PMID: 12359984
  34. Both the ACE and chymase-like enzyme activities in the aneurysmal aortae were significantly higher than those in the control aortae. PMID: 12484503
  35. Degradation of phospholipid transfer protein (PLTP) and PLTP-generated pre-beta-high density lipoprotein by this enzyme in mast cells impairs high affinity efflux of cholesterol from macrophage foam cells. PMID: 12531890
  36. Interactions among the loops bordering and defining the active site appear to influence both the zymogen and the activated conformations of chymase in this model. PMID: 12614156
  37. albumin is a substrate of human chymase PMID: 12815038
  38. new class of chymase inhibitor through a substituent analysis of MWP00965 chemically synthesized PMID: 14592513
  39. chymase depletes pre-beta-high density lipoprotein, which impairs ATP-binding cassette transporter A1- but not scavenger receptor class B type I-mediated lipid efflux to high density lipoprotein PMID: 14701812
  40. MCT1 immunoreactivity was visible in blood vessel walls as early as the 13th week of gestation mainly in the visual cortical plate and subplate. PMID: 14757520
  41. mast cell chymase-1 is unlikely to influence blood pressure levels in the Japanese population. PMID: 15106801
  42. Bladder fibrosis may be mediated by mast cell chymase stimulation of collagen synthesis. PMID: 15227657
  43. The synthesis of new potential inhibitors of human chymase is described PMID: 15449728
  44. Tryptase and chymase and protein levels were determined in mast cells in fibrosarcoma. PMID: 15638376
  45. mast cell chymase activates ERK and p38 probably through G-protein-coupled receptor, and the ERK but not p38 cascade may have a crucial role in chymase-induced migration of eosinophils PMID: 15919053
  46. A novel (TG)n(GA)m repeat polymorphism 254 bp downstream of the mast cell chymase (CMA1) gene is associated with atopic asthma and total serum IgE levels. PMID: 15924217
  47. Chymase-induced apoptosis of conjunctival epithelial cells represents anoikis, which is a slowly occurring apoptotic process induced by lack of adhesion to an extracellular matrix. PMID: 16020275
  48. Significant association between the CMA1 promoter polymorphism rs1800875 and atopic eczema supports the hypothesis that CMA1 serves as candidate gene for atopic eczema. PMID: 16134991
  49. activated human skin mast cells (MCs) convert CTAP-III into biologically active NAP-2 through proteolytic cleavage by released chymase. PMID: 16317101
  50. The CMA1 polymorphisms studied do not contribute to disease susceptibility in Japanese or Dutch sarcoidosis patients. PMID: 16446531


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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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