Recombinant Human CHL1 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-1246
Recombinant Human CHL1 Protein (His Tag)

Recombinant Human CHL1 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-1246
Catalog No.: BLPSN-1246

Product Overview

Tag His
Host Species Human
Accession AAI04919.1
Synonym CALL, L1CAM2, LICAM2
Background Neural cell adhesion molecule L1-like protein, also known as close homolog of L1 (CHL1) is the prototypic member of the CTF / NF-1 family of transcription factors that serve as a novel calcium signaling pathway-responsive transcription factor and is considered as a member of the largest ctf complementation group, consisting of 3 of 126 ctf mutants isolated. CHL1 is a cell adhesion molecule highly related to L1. It contains structure plan of six extracellular C2-type immunoglobulin (Ig) domains followed by five fibronectin type-…¢ domains linked by a single membrane-spanning region to a short cytoplasmic domain. The extracellular portion of CHL1 is higyly glycosylated and involved them in hemophilic disease.
Description A DNA sequence encoding the extracellular domain of human CHL1 (AAI04919.1) (Met 1-Gln 1080) was fused with a His tag at the C-terminus.
Source HEK293
Predicted N Terminal Ile 25
AA Sequence Met 1-Gln 1080
Molecular Weight The recombinant human CHL1 consists of 1067 a.a. after removal of the signal peptide and predicts a molecular mass of 120 kDa. As a result of glycosylation, the apparent molecular mass of rhCHL1 is approximately 160-180 kDa in SDS-PAGE under non-reduced conditions.
Purity >95% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells. When 5 x 10E4 cells/well are added to CHL1 coated plates (0.8 ug/ml and 100 ul/well), approximately 40%-60% will adhere specifically after 60 minutes at 37°C.
Formulation Lyophilized from sterile PBS, pH 7.4.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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