Recombinant Human CHL1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1246
Recombinant Human CHL1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1246
Catalog No.: BLPSN-1246
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | AAI04919.1 |
| Synonym | CALL, L1CAM2, LICAM2 |
| Background | Neural cell adhesion molecule L1-like protein, also known as close homolog of L1 (CHL1) is the prototypic member of the CTF / NF-1 family of transcription factors that serve as a novel calcium signaling pathway-responsive transcription factor and is considered as a member of the largest ctf complementation group, consisting of 3 of 126 ctf mutants isolated. CHL1 is a cell adhesion molecule highly related to L1. It contains structure plan of six extracellular C2-type immunoglobulin (Ig) domains followed by five fibronectin type-…¢ domains linked by a single membrane-spanning region to a short cytoplasmic domain. The extracellular portion of CHL1 is higyly glycosylated and involved them in hemophilic disease. |
| Description | A DNA sequence encoding the extracellular domain of human CHL1 (AAI04919.1) (Met 1-Gln 1080) was fused with a His tag at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Ile 25 |
| AA Sequence | Met 1-Gln 1080 |
| Molecular Weight | The recombinant human CHL1 consists of 1067 a.a. after removal of the signal peptide and predicts a molecular mass of 120 kDa. As a result of glycosylation, the apparent molecular mass of rhCHL1 is approximately 160-180 kDa in SDS-PAGE under non-reduced conditions. |
| Purity | >95% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells. When 5 x 10E4 cells/well are added to CHL1 coated plates (0.8 ug/ml and 100 ul/well), approximately 40%-60% will adhere specifically after 60 minutes at 37°C. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
