Recombinant Human CHGC Protein (C-6His)

Name: Recombinant Human CHGC Protein (C-6His)
Brand: Beta LifeScience
SKU: BL-0767NP
Availability: InStock

BL-0767NP: Greater than 90% as determined by reducing SDS-PAGE. (QC verified)

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Secretogranin-2/Chromogranin-C is produced by our Mammalian expression system and the target gene encoding Ser29-Met617 is expressed with a 6His tag at the C-terminus.
Accession	AAH22509.1
Synonym	Secretogranin-2; Chromogranin-C; Secretogranin II; SgII
Gene Background	Secretogranin-2 is also known as Chromogranin-C,Secretogranin II, SgII. In humans, it is encoded by the SCG2 gene.It belongs to the chromogranin/secretogranin protein family. Secretogranin-2 is a neuroendocrine secretory granule protein, which is the precursor for biologically active peptides. It derived secretoneurin was distributed with strong immunoreactivity in the somata of pelvic ganglion neurons,72% of which also contained tyrosine hydroxyldse,as well as in nerve terminals in the muscular layer and the lamina propria of the vas deferens.
Molecular Mass	68.9 KDa
Apmol Mass	85-95 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 90% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Neuroendocrine protein of the granin family that regulates the biogenesis of secretory granules.
Subcellular Location	Secreted. Note=Neuroendocrine and endocrine secretory granules.
Protein Families	Chromogranin/secretogranin protein family
Database References	HGNC: 10575 OMIM: 118930 KEGG: hsa:7857 STRING: 9606.ENSP00000304133 UniGene: PMID: 26378181 present data show that SgII is highly expressed in advanced prostate cancer and may contribute to the neuroendocrine differentiation by promoting the formation of secretory granules and the proliferation of PCa cells. PMID: 25307750 SN induces MUC5AC hypersecretion in a dose- and time-dependent manner; moreover, the MUC5AC over synthesis induced by SN is strongly associated with the enhanced binding of EGF to NRP1 PMID: 24556756 Topical secretoneurin gene therapy accelerates diabetic wound healing by interaction between heparan-sulfate proteoglycans and basic FGF. PMID: 23918206 Manserin may serve as a marker of prostate cancer progression. PMID: 21803620 CgA, CgB, and secretoneurin are detectable in feces, and collagenous colitis patients express higher values than patients with inflammatory bowel disease and controls. In treatment, fecal secretoneurin decreased to control levels in collagenous colitis. PMID: 23423580 In vivo secretoneurin improves left ventricular function, inhibits remodeling, and reduces scar formation; in the infarct border zone, secretoneurin induces coronary angiogenesis. PMID: 23081990 Circulating Levels of SgII are Increased in Patients with chronic, stable heart failure. PMID: 22655045 Data describe the gene expression and protein production of SgII in normal adrenal glands and pheochromocytomas with the goal to examine the molecular mechanisms leading to the marked variations in the expression of EM66 in tumoral chromaffin tissue. PMID: 22217803 This short review deals with investigations in neuroendocrine tumors (NETs) with antibodies against defined epitopes of chromogranins (Cgs) A and B and secretogranins (Sgs) II and III. PMID: 21046454 More SgII immunoreactive cells were observed in phaeochromocytomas. PMID: 20550951 Transendothelial migration of leukocytes and signalling mechanisms in response to the neuropeptide secretoneurin. PMID: 11853870 secretogranin II-derived peptide EM66 generated in human tumoral chromaffin tissue; significant difference in EM66 concentrations between benign and malignant pheochromocytomas PMID: 12788858 the high concentration of secretoneurin in the aqueous humor indicates a significant role of this peptide PMID: 15572199 a variant of Secretogranin II has a role in regulation by PHOX2 transcription factors and in hypertension PMID: 17584765 Results suggest that secretogranin II represents a key AP-1-regulated protein that counteracts nitric oxide toxicity and mediates neuronal differentiation of neuroblastoma cells. PMID: 18239671 Increased concentrations of SgII, especially the N-terminal part of secretoneurin could be measured in plasma from patients with endocrine pancreatic tumours. PMID: 18448176 Suppression of Pdcd4 resulted in an increased release of CgA and Sg II and was accompanied by an up-regulation of intracellular PC1. PMID: 18549351 semiquantitative immunocytochemistry for secretogranin II in amyotrophic lateral sclerosis. PMID: 18721831

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.