Recombinant Human CCL26 Protein (71AA)

Name: Recombinant Human CCL26 Protein (71AA)
Brand: Beta LifeScience
SKU: BL-2037NP
Availability: InStock

BL-2037NP: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)

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Product Overview

Description	Recombinant Human C-C Motif Chemokine 26 is produced by our E.coli expression system and the target gene encoding Thr24-Leu94 is expressed.
Accession	Q9Y258
Synonym	C-C Motif Chemokine 26; CC Chemokine IMAC; Eotaxin-3; Macrophage Inflammatory Protein 4-Alpha; MIP-4-Alpha; Small-Inducible Cytokine A26; Thymic Stroma Chemokine-1; TSC-1; CCL26; SCYA26
Gene Background	Chemokine Ligand 26 protein (CCL26) is a novel small cytokine belonging to the CC chemokine family which is involved in immunoregulatory and inflammatory processes. CCL26 is constitutively expressed in thymus, but only transiently expressed in phytohemagglutinin-stimulated peripheral blood mononuclear cells. It specifically binds and induces chemotaxis in T cells and elicits its effects by interacting with the chemokine receptor CCR4. CCL26, along with Eotaxin-1 and Eotaxin-2, selectively activates the CC chemokine receptor 3 (CCR3). The Eotaxin-3-CCR3 interaction may play an important role in allergic diseases such as atopic dermatitis and bronchial asthma. The full-length cDNA for CCL26 encodes a protein of 94 amino acids with a putative signal peptide of either 23 or 26 amino acid residues. Both the 71 and 68 amino acid residue variants of recombinant CCL26 demonstrate equal potency in inducing chemotaxis of a human CCR3-transfected cell line. Unlike most other CC chemokines, CCL26 maps to human chromosome 7q11.2, within 40 kilobases of the Eotaxin-2 loci. CCL26 and Eotaxin-2 are unique in that they are the only chemokines identified to date that map to chromosome 7.
Molecular Mass	8.53 KDa
Apmol Mass	13 KDa, reducing conditions
Formulation	Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 1mM EDTA, 20% Glycerol, pH 9.0.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution
Storage	Store at ≤-70°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
Shipping	The product is shipped on dry ice/polar packs.Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Chemoattractant for eosinophils and basophils. Acts as a ligand for C-C chemokine receptor CCR3 which triggers Ca(2+) mobilization in eosinophils. Also acts as a ligand for CX3C chemokine receptor CX3CR1, inducing cell chemotaxis.
Subcellular Location	Secreted.
Protein Families	Intercrine beta (chemokine CC) family
Database References	HGNC: 10625 OMIM: 604697 KEGG: hsa:10344 STRING: 9606.ENSP00000005180 UniGene: PMID: 28089872 hypoxia-inducible factors activate the transcription of chemokine ligand 26 in cancer cells to recruit chemokine receptor 1-expressing myeloid-derived suppressor cells to the primary tumor PMID: 27228567 this study shows that expression of CCL26 is increased in eosinophilic myocarditis patients compared to chronic lymphocytic myocarditis patients PMID: 27621211 The reduction of circulating levels of MCP-4, eotaxin-3, and MIP-1beta could be one of the mechanisms by which bariatric surgery contributes to the reduction of cardiovascular risk in these patients. PMID: 27300476 Review: eotaxins (CCL11, CCL24, and CCL26) play key role(s) during symptomatic inflammatory responses raised in response to allergic crisis of allergic asthma and atopic dermatitis PMID: 26861136 Data show that the interleukins IL-4 and IL-13 are critical factors for the induction of eotaxin-3/CCL26 in the pancreas. PMID: 26418908 These results show a relation between CCL26 production by IL-13-stimulated bronchial epithelial cells , sputum eosinophil counts, and asthma severity. They also suggest a role for CCL26 in the sustained inflammation observed in patients with severe eosinophilic asthma and reveal CCL26 as a potential target for treating patients with eosinophilic asthma that are refractory PMID: 25936567 The most potent inhibition of RANTES, eotaxin and eotaxin3 as well as MMP9 activity. PMID: 25323950 These findings indicate that eotaxin-3 is expressed in Chronic subdural hematoma fluid PMID: 24684589 these data suggest that CCL26 and CCL24 are likely involved in the pathogenesis of chronic nasal hypereosinophilia, with a complex cooperation and different involvement of the various members of eotaxin family. PMID: 24989688 CCL11, CCL24, and CCL26 are increased in TB patients; hence, it seems that TB suppresses Th1 and the classic function of macrophages subsequently by inducing the chemokines' expression PMID: 24600981 these data identify a contributory role for DNA methylation in regulating eotaxin-3 production in human allergic inflammation. PMID: 24323578 CCL11, CCL24, and CCL26 has a role in the recruitment of extravillous trophoblast into decidual tissue and vessels. PMID: 23477905 CCL26 is a more effective chemoattractant than CCL11 or CCL24 for eosinophils of asthmatics. All eotaxins induced the eosinophil migration from 0 to 6 h, but CCL26 triggered a second phase of migration between 12 & 18 h. PMID: 23532518 CCL1, CCL26, and IgE may be associated with pruritus in cutaneous T-cell lymphoma. PMID: 22948508 elevated expression in the active lesions of ulcerative colitis and Crohn's disease PMID: 23607908 Oesophageal squamous cells from gastro-oesophageal reflux disease and Eosinophilic oesophagitis patients express similar levels of eotaxin-3 when stimulated by Th2 cytokines, and omeprazole blocks that eotaxin-3 expression. PMID: 22580413 PPIs, in concentrations achieved in blood with conventional dosing, significantly inhibit IL-4-stimulated eotaxin-3 expression in EoE esophageal cells and block STAT6 binding to the promoter. PMID: 23185525 Up-regulation of CCL2, CCL26, IL6 and LOXL2 genes in cancer cells are part of the common effects of cancer-associated fibroblasts on hepatocellular carcinoma cells PMID: 22739041 Phosphodiesterase 4 inhibitors, rolipram and RO-20-1724 have no effect on CCL26 expression in human primary bronchial epithelial cells. PMID: 22946025 Leukotriene D4 and interleukin-13 cooperate to increase the release of eotaxin-3 by airway epithelial cells PMID: 22952702 The level of eotaxin expression and inflammatory cell count were measured in the material from nasal brushing in healthy controls and in patients with allergic rhinitis, asthma, and chronic obstructive pulmonary disease. PMID: 22846146 Data show that that IL-4 signals through the Jak1, 2/Stat6 pathway in keratinocytes to stimulate CCL26 expression and this may provide an explanation for the pathogenesis of atopic dermatitis (AD). PMID: 22226123 CCL26 but not CCL24 was elevated in bullous pemphigoid lesions. PMID: 21881593 The expression of AMCase, eotaxin-3, IL-13, and mRNA was significantly higher in patients with CRSwNP than in the control group. PMID: 21493274 these data link the up-regulation of the eosinophil chemotactic factor CCL26 in bullous pemphigoid to the lesional accumulation of activated eosinophils in the skin. PMID: 21985360 Serum eotaxin-3 is a sensitive and specific marker for the diagnosis of active Churg-Strauss syndrome suitable for routine clinical practice. PMID: 21266446 CCL26-directed small-interfering RNA (siRNA) treatments significantly decreased release of CCL5 (RANTES), CCL15 (MIP-1delta), CCL8 (MCP-2), and CCL13 (MCP-4). PMID: 19203252 Epigenetic regulation of the IL-13-induced human eotaxin-3 gene by CREB-binding protein-mediated histone 3 acetylation. PMID: 21325281 AMCase and eotaxin-3 may be important mediators in the pathogenesis of nasal polyps. The increased AMCase and eotaxin-3 might lead to nasal polyp formation and growth. PMID: 21303604 CCL26 is an agonist for CX3CR1 and may play a dual role in allergic diseases by attracting eosinophils via CCR3 and killer lymphocytes and resident monocytes via chemokine receptor CX3CR1. PMID: 20974991 Serum CCL11 was increased in ulcerative colitis (UC) and less in Crohn's disease (CD), whereas CCL24 and CCL26 were increased only in UC. Colon expression of the CCL's was higher in UC vs. CD, and was induced by Th2 cytokines in colon epithelial cells. PMID: 21077277 interaction of eotaxins and CCR3 regulates the Th2-dominant tumor environment, which is closely related to the development of cutaneous T-cell lymphoma PMID: 20505746 The mean gene expression level for CCL11, CCL24, CCL26 was higher in skin changes of atopic dermatitis patients than in uninvolved skin. PMID: 20236835 data show that IL-4 and pro-inflammatory cytokines such as TNF-alpha, IL-1beta and IFN-gamma regulate CCL26 synthesis in human monocytic cells PMID: 20059579 promotes lung fibroblast migration PMID: 20143648 Expression is modulated by cytokines and glucocorticoids. PMID: 12061839 Pretreatment or co-treatment with each of the eotaxins augmented PMAtate-induced superoxide generation, concentration-dependent degranulation of eosinophil peroxidase, & potentiation of A23187-induced degranulation. PMID: 12192108 eotaxin-3 inhibits MCP-1-mediated responses, thus acting as a natural antagonist for CCR2, and promotes active movement of monocytes away from a gradient of eotaxin-3 PMID: 12689946 eotaxin-3 is the first human chemokine that features broadband antagonistic activities; may have a modulatory rather than an inflammatory function;may play an unrecognized role in the polarization of cellular recruitment by attracting Th2 lymphocytes PMID: 15039444 results suggest that types 1 and 2 IL-4 receptors and nuclear factor-kappaB may have a role in eotaxin-3 production in bronchial epithelial cells PMID: 15521376 CCL26 is major eotaxin synthesized and released by alveolar epithelial cells and is involved in autoregulation of CCR3 receptors and other eotaxins. This CCL26-CCR3 ligand-receptor system may be an attractive target in therapy of airway inflammation. PMID: 15863444 results demonstrate that epithelial eotaxin-3 is up-regulated in the context of a T helper 2 mediated inflammatory bowel disease via the signal transducer and activator of transcription 6 PMID: 16084752 In conclusion, these results suggest that viral airway infection may enhance interleukin-4-induced eotaxin-3 production through upregulation of the interleukin-4 receptor in airway epithelial cells. PMID: 16264039 association of eotaxin-3 polymorphisms in ulcerative colitis in Korean patients PMID: 16391516 results implicate eotaxin-3 as a critical effector molecule for eosinophilic esophagitis and provide insight into disease pathogenesis PMID: 16453027 Contributes to dermal and epidermal eosinophil infiltration in Th2-polarized skin inflammation in which interleukin-4 is produced. PMID: 17073866 Data suggest atherosclerotic inflammation may be a trigger for sclerosis in calcified stenotic aortic valves through upregulation TGF-beta, VAP-1, MIG and Eotaxin3, which is only partially inhibited by previous statin therapy. PMID: 17490641 The Th2 cytokine IL-4 preferentially stimulated eotaxin-3 expression. PMID: 17541284 Determination of eotaxin-3 levels by real-time polymerase chain reaction on paraffinized, formalin-fixed tissue may be a useful test in the differentiation of eosinophilic esophagitis from gastroesophageal reflux disease PMID: 17900656

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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Recombinant Human CCL26 Protein (71AA)

Recombinant Human CCL26 Protein (71AA)

Product Overview

Target Details

FAQs