Recombinant Human Catalase Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-0593
Recombinant Human Catalase Protein (His Tag)

Recombinant Human Catalase Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-0593
Catalog No.: BLPSN-0593

Product Overview

Tag His
Host Species Human
Accession P04040
Background Catalase is a ubiquitously expressed enzyme that catalyzes the decomposition of hydrogen peroxide to water and oxygen. It is a tetramer of four polypeptides chains containing four porphyrin heme groups that allow the enzyme to react with the hydrogen peroxide. The optimum PH of human catalase is approximately 7 and the optimum temperature is at 37 degree. Both the PH optimum and temperature for other catalases varies depending on the species. Catalase can be inhibited by a flux of O2- generated in situ by the aerobic xanthine oxidase reaction. This inhibition of catalase by O2- provides the basis for a synergism between superoxide dismutase and catalase.Such synergisms have been observed in vitro and may be significant in vivo. Catalase is used in the food industry for removing hydrogen peroxide from milk prior to cheese production. Another use is in food wrappers where it prevents food from oxidizing. Catalase is also used in the textile industry, removing hydrogen peroxide from fabrics to make sure the material is peroxide-free.
Description A DNA sequence encoding the human CAT (P04040) (Ala 2-Leu 527) was expressed, with a His tag at the N-terminus.
Source Baculovirus-Insect Cells
Predicted N Terminal Met
AA Sequence Ala 2-Leu 527
Molecular Weight The recombinant human CAT consists of 545 a.a. and predicts a molecular mass of 61.9 kDa. It migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions.
Purity >80% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Please contact us for detailed information
Formulation Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 10% gly.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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