Recombinant Human Casein Kinase 1 alpha Protein (GST Tag)

Beta LifeScience SKU/CAT #: BLPSN-0585

Recombinant Human Casein Kinase 1 alpha Protein (GST Tag)

Beta LifeScience SKU/CAT #: BLPSN-0585
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Product Overview

Host Species Human
Accession P48729
Synonym CK1, CK1a, CKIa, HEL-S-77p, HLCDGP1, PRO2975
Background Casein kinase I isoform alpha, also known as CKI-alpha, CK1 and CSNK1A1, is a cytoplasm protein which belongs to theprotein kinase superfamily, CK1 Ser/Thr protein kinase family and casein kinase I subfamily. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. High expression of CSNK2A1, or concomitantly high expression of CSNK2A1, are independent prognostic factors of poor survival in NSCLC patients. CSNK2A1 are useful prognosis markers in non-small cell lung cancer (NSCLC) patients after complete resection, independent of lymph node metastasis status. CSNK1A1 can phosphorylate a large number of proteins. It participates in Wnt signaling. It phosphorylates CTNNB1 at 'Ser-45'. CSNK1A1 may play a role in segregating chromosomes during mitosis.
Description A DNA sequence encoding the full length of human CSNK1A1 isoform 1 (P48729-1) (Met 1-Phe 337) was expressed with the GST tag at the N-terminus.
Source Baculovirus-Insect Cells
Predicted N Terminal Met
AA Sequence Met 1-Phe 337
Molecular Weight The recombinant human CSNK1A1/GST chimera consists of 561 a.a. and predicts a molecular mass of 65.2 kDa. It migrates as an approximately 62 kDa band in SDS-PAGE under reducing conditions.
Purity >90% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity The specific activity was determined to be 30 nmol/min/mg using casein as substrate.
Formulation Supplied as sterile 20mM Tris, 500mM NaCl, 2mM GSH, pH 7.4.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis. May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration.
Subcellular Location Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Nucleus speckle. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, spindle.
Protein Families Protein kinase superfamily, CK1 Ser/Thr protein kinase family, Casein kinase I subfamily
Database References

HGNC: 2451

OMIM: 600505

KEGG: hsa:1452

UniGene: Hs.529862

Gene Functions References

  1. Study reports that CK1alpha similarly destabilizes FOXO4 in RAS-mutant cells by phosphorylation at serines 265/268. PMID: 28945225
  2. Data indicate that casein kinase 1 alpha (CK1alpha) RNA levels might serve as prognostic marker for colorectal cancer (CRC). PMID: 29409464
  3. cellular kinase, casein kinase 1alpha (CK1alpha), is crucial for IAV HA-induced degradation of both IFNGR1 and IFNAR1. PMID: 29343571
  4. casein kinase 1 phosphorylates the SQSTM1 S349 residue when harmful proteins accumulate under HSF1 stress PMID: 27846364
  5. Maintenance of the progenitors thus requires cooperation by PRMT1 and CSNK1a1 to sustain proliferation gene expression and suppress premature differentiation driven by GRHL3 PMID: 28943242
  6. These data indicate that CK1alpha has a dominant and non-redundant function in melanoma cells PMID: 27488834
  7. Disruption of CK1alpha function in myeloma cells resulted in decreased Mdm2, increased p53 and p21 and reduced expression of beta-catenin and AKT. PMID: 28099937
  8. CK1delta enhances EPO secretion from liver cancer cells under hypoxia by modifying HIF-2alpha and promoting its nuclear accumulation. PMID: 27686097
  9. CKIalpha-mediated NS5A S235 phosphorylation is critical for HCV replication. CaMKII gamma and delta may have negative roles in the HCV life cycle. PMID: 27875595
  10. Interactome analyses revealed that the Jade-1S mutant unable to be phosphorylated by CK1alpha has an increased binding affinity to proteins involved in chromatin remodelling, histone deacetylation, transcriptional repression, and ribosome biogenesis. PMID: 26919559
  11. study focuses on, how the structural dynamics and conformational changes of two CK1 isoforms are synchronized in carcinogenic pathway PMID: 26788877
  12. NIFK is required for lung cancer progression via the RUNX1-dependent CK1alpha repression, which activates TCF4/beta-catenin signaling in metastasis and the Ki-67-dependent regulation in cell proliferation. PMID: 26984280
  13. The study describes the identification of a novel, tumor-specific missense mutation in the active site of casein kinase 1 alpha(CSNK1A1) using activity-based proteomics. PMID: 27031502
  14. characterization of frequency and potential clinical impact of CSNK1A1 mutations in myelodysplastic syndrome(MDS) and acute myeloid leukemia following MDS in a large cohort of patients PMID: 25792355
  15. The dynamical and conformational properties for each of three isoforms of CK1 are explored through molecular dynamics (MD) simulations. PMID: 25665722
  16. Phosphorylation of LRRK2 by casein kinase 1alpha regulates trans-Golgi clustering via differential interaction with ARHGEF7. PMID: 25500533
  17. Casein kinase 1alpha is a key negative regulator of oncogenic RAS-induced autophagy. Depletion or pharmacologic inhibition of CK1alpha enhanced autophagic flux in oncogenic RAS-driven human fibroblasts and multiple cancer cell lines. PMID: 25798617
  18. CSNK1alpha1 has a role in mediating malignant plasma cell survival PMID: 24962017
  19. Casein kinase 1 alpha specifically phosphorylates Jade-1 at an unprimed SLS phosphorylation site. PMID: 25100726
  20. Degradation of Tiam1 by casein kinase 1 and the SCFbetaTrCP ubiquitin ligase controls the duration of mTOR-S6K signaling. PMID: 25124033
  21. Data indicate that protein S phosphorylation by casein kinase 1 (CK1) and casein kinase 2 (CK2) increased activated proten C cofactor activity. PMID: 23580615
  22. The substrate interacting residues in ck1alpha have been identified using the structural model of kinase - substrate peptide. The PKR interacting NS5A 1b residues have also been predicted. PMID: 23148689
  23. DNA damage activates p53 in part by disrupting CK1a-MDMX interaction and reducing MDMX-p53 binding affinity. PMID: 23028042
  24. identified casein kinase 1alpha (CK1alpha) as a direct target of miR-155 control which enhanced beta-catenin signaling and cyclin D1 expression, promoting tumor cell growth PMID: 22350414
  25. We showed that just four genes, G3BP2, SCARB2, CSNK1A1 and SPRR2B, can classify patients as presence of lymph node metastasis negative or positive, with 80.0% accuracy. PMID: 21985131
  26. mTOR cooperates with CK1alpha and betaTrCP to generate an auto-amplification loop to promote its own full activation. PMID: 22017877
  27. The authors characterized the role of CKIalpha in the clock mechanism and found that CKIalpha-mediated phosphorylation stimulated degradation of a clock protein PER1, similar to the function of CKIdelta. PMID: 21179498
  28. Our results indicate that the nuclear form of CK1alpha in humans, CK1alphaLS, plays a critical role in vascular cell proliferation, cellular activation, and hydrogen peroxide-mediated mitogenic signal transduction. PMID: 20696773
  29. Casein kinase 1 functions as both penultimate and ultimate kinase in regulating Cdc25A destruction. PMID: 20348946
  30. Biochemical and cellular characteristics of the four splice variants of protein kinase CK1alpha from zebrafish (Danio rerio)are presented. PMID: 12210746
  31. CK1alpha can promote cell survival by interfering with RXR agonist-induced apoptosis PMID: 15131121
  32. CK1alpha-mediated phosphorylation modulates the mRNA binding ability of hnRNP-C PMID: 15687492
  33. NFAT1, a transcription factor connected with breast cancer metastasis, is activated by Wnt-5a through a Ca2+ signaling pathway in human breast epithelial cells which was simultaneously counteracted by a Wnt-5a-induced Yes/Cdc42 signaling pathway. PMID: 16880514
  34. identification of the alpha isoform of casein kinase I as the cellular kinase important for the formation of hepatitis C virus (HCV) NS5A-p58; here we show the effects of this kinase on NS5A hyperphosphorylation and HCV replication PMID: 16943283
  35. CK1alphaLS and hnRNP-C represent conserved components of a vertebrate-specific H(2)O(2)-responsive nuclear signaling pathway. PMID: 17626781
  36. Studies suggest that CK1alpha is another kinase that regulates RIP1 function in NF-kappaB activation. PMID: 18067272
  37. following DNA damage, PML facilitates Thr18 phosphorylation by recruiting p53 and CK1 into PML nuclear bodies, thereby protecting p53 from inhibition by Mdm2, leading to p53 activation. PMID: 18246126
  38. Ser-155 and Ser-158 have important roles in the nucleocytoplasmic trafficking of OREBP/TonEBP and CK1 plays a major role in regulating this process PMID: 18411282
  39. We show that the inhibition of CK1 activates RhoB and promotes RhoB dependent actin fiber formation and EGF-R level. PMID: 18590726
  40. CK1 has a role as the Ser20 site kinase for p53 in DNA virus-infected cells PMID: 18669630
  41. Phosphorylation of parkin by casein kinase I and cdk5 decreases parkin solubility, leading to its aggregation and inactivation. PMID: 19050041
  42. 'activated B-cell-like' subtype of diffuse large B-cell lymphoma cells required CK1alpha for constitutive NF-kappaB activity, indicating that CK1alpha functions as a conditionally essential malignancy gene PMID: 19118383
  43. Casein Kinase 1 phosphorylates serine 56 of yellow fever virus methyltransferase. PMID: 19185594
  44. Data show that Wnt-5a stimulation of breast epithelial cells leads to increased cell-cell adhesion, and that Wnt-5a/casein kinase Ialpha (CKIalpha)-specific phosphorylation of beta-catenin leads to increased complex formation of beta-catenin/E-cadherin. PMID: 19244247
  45. CK1alpha plays a central role in mediating MDM2 control of p53 and E2F-1 protein stability PMID: 19759023
  46. Results suggest a role for mammalian and parasite casein kinase 1alpha in regulating IFNAR1 stability and type I IFN signaling. PMID: 19805514


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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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