Recombinant Human Casein Kinase 1 alpha Protein (GST Tag)

Name: Recombinant Human Casein Kinase 1 alpha Protein (GST Tag)
Brand: Beta LifeScience
SKU: BLPSN-0585
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Tag	GST
Host Species	Human
Accession	P48729
Synonym	CK1, CK1a, CKIa, HEL-S-77p, HLCDGP1, PRO2975
Background	Casein kinase I isoform alpha, also known as CKI-alpha, CK1 and CSNK1A1, is a cytoplasm protein which belongs to theprotein kinase superfamily, CK1 Ser/Thr protein kinase family and casein kinase I subfamily. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. High expression of CSNK2A1, or concomitantly high expression of CSNK2A1, are independent prognostic factors of poor survival in NSCLC patients. CSNK2A1 are useful prognosis markers in non-small cell lung cancer (NSCLC) patients after complete resection, independent of lymph node metastasis status. CSNK1A1 can phosphorylate a large number of proteins. It participates in Wnt signaling. It phosphorylates CTNNB1 at 'Ser-45'. CSNK1A1 may play a role in segregating chromosomes during mitosis.
Description	A DNA sequence encoding the full length of human CSNK1A1 isoform 1 (P48729-1) (Met 1-Phe 337) was expressed with the GST tag at the N-terminus.
Source	Baculovirus-Insect Cells
Predicted N Terminal	Met
AA Sequence	Met 1-Phe 337
Molecular Weight	The recombinant human CSNK1A1/GST chimera consists of 561 a.a. and predicts a molecular mass of 65.2 kDa. It migrates as an approximately 62 kDa band in SDS-PAGE under reducing conditions.
Purity	>90% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	The specific activity was determined to be 30 nmol/min/mg using casein as substrate.
Formulation	Supplied as sterile 20mM Tris, 500mM NaCl, 2mM GSH, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May phosphorylate PER1 and PER2. May play a role in segregating chromosomes during mitosis. May play a role in keratin cytoskeleton disassembly and thereby, it may regulate epithelial cell migration.
Subcellular Location	Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Nucleus speckle. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, spindle.
Protein Families	Protein kinase superfamily, CK1 Ser/Thr protein kinase family, Casein kinase I subfamily
Database References	HGNC: 2451 OMIM: 600505 KEGG: hsa:1452 UniGene: Hs.529862

Gene Functions References

Study reports that CK1alpha similarly destabilizes FOXO4 in RAS-mutant cells by phosphorylation at serines 265/268. PMID: 28945225
Data indicate that casein kinase 1 alpha (CK1alpha) RNA levels might serve as prognostic marker for colorectal cancer (CRC). PMID: 29409464
cellular kinase, casein kinase 1alpha (CK1alpha), is crucial for IAV HA-induced degradation of both IFNGR1 and IFNAR1. PMID: 29343571
casein kinase 1 phosphorylates the SQSTM1 S349 residue when harmful proteins accumulate under HSF1 stress PMID: 27846364
Maintenance of the progenitors thus requires cooperation by PRMT1 and CSNK1a1 to sustain proliferation gene expression and suppress premature differentiation driven by GRHL3 PMID: 28943242
These data indicate that CK1alpha has a dominant and non-redundant function in melanoma cells PMID: 27488834
Disruption of CK1alpha function in myeloma cells resulted in decreased Mdm2, increased p53 and p21 and reduced expression of beta-catenin and AKT. PMID: 28099937
CK1delta enhances EPO secretion from liver cancer cells under hypoxia by modifying HIF-2alpha and promoting its nuclear accumulation. PMID: 27686097
CKIalpha-mediated NS5A S235 phosphorylation is critical for HCV replication. CaMKII gamma and delta may have negative roles in the HCV life cycle. PMID: 27875595
Interactome analyses revealed that the Jade-1S mutant unable to be phosphorylated by CK1alpha has an increased binding affinity to proteins involved in chromatin remodelling, histone deacetylation, transcriptional repression, and ribosome biogenesis. PMID: 26919559
study focuses on, how the structural dynamics and conformational changes of two CK1 isoforms are synchronized in carcinogenic pathway PMID: 26788877
NIFK is required for lung cancer progression via the RUNX1-dependent CK1alpha repression, which activates TCF4/beta-catenin signaling in metastasis and the Ki-67-dependent regulation in cell proliferation. PMID: 26984280
The study describes the identification of a novel, tumor-specific missense mutation in the active site of casein kinase 1 alpha(CSNK1A1) using activity-based proteomics. PMID: 27031502
characterization of frequency and potential clinical impact of CSNK1A1 mutations in myelodysplastic syndrome(MDS) and acute myeloid leukemia following MDS in a large cohort of patients PMID: 25792355
The dynamical and conformational properties for each of three isoforms of CK1 are explored through molecular dynamics (MD) simulations. PMID: 25665722
Phosphorylation of LRRK2 by casein kinase 1alpha regulates trans-Golgi clustering via differential interaction with ARHGEF7. PMID: 25500533
Casein kinase 1alpha is a key negative regulator of oncogenic RAS-induced autophagy. Depletion or pharmacologic inhibition of CK1alpha enhanced autophagic flux in oncogenic RAS-driven human fibroblasts and multiple cancer cell lines. PMID: 25798617
CSNK1alpha1 has a role in mediating malignant plasma cell survival PMID: 24962017
Casein kinase 1 alpha specifically phosphorylates Jade-1 at an unprimed SLS phosphorylation site. PMID: 25100726
Degradation of Tiam1 by casein kinase 1 and the SCFbetaTrCP ubiquitin ligase controls the duration of mTOR-S6K signaling. PMID: 25124033
Data indicate that protein S phosphorylation by casein kinase 1 (CK1) and casein kinase 2 (CK2) increased activated proten C cofactor activity. PMID: 23580615
The substrate interacting residues in ck1alpha have been identified using the structural model of kinase - substrate peptide. The PKR interacting NS5A 1b residues have also been predicted. PMID: 23148689
DNA damage activates p53 in part by disrupting CK1a-MDMX interaction and reducing MDMX-p53 binding affinity. PMID: 23028042
identified casein kinase 1alpha (CK1alpha) as a direct target of miR-155 control which enhanced beta-catenin signaling and cyclin D1 expression, promoting tumor cell growth PMID: 22350414
We showed that just four genes, G3BP2, SCARB2, CSNK1A1 and SPRR2B, can classify patients as presence of lymph node metastasis negative or positive, with 80.0% accuracy. PMID: 21985131
mTOR cooperates with CK1alpha and betaTrCP to generate an auto-amplification loop to promote its own full activation. PMID: 22017877
The authors characterized the role of CKIalpha in the clock mechanism and found that CKIalpha-mediated phosphorylation stimulated degradation of a clock protein PER1, similar to the function of CKIdelta. PMID: 21179498
Our results indicate that the nuclear form of CK1alpha in humans, CK1alphaLS, plays a critical role in vascular cell proliferation, cellular activation, and hydrogen peroxide-mediated mitogenic signal transduction. PMID: 20696773
Casein kinase 1 functions as both penultimate and ultimate kinase in regulating Cdc25A destruction. PMID: 20348946
Biochemical and cellular characteristics of the four splice variants of protein kinase CK1alpha from zebrafish (Danio rerio)are presented. PMID: 12210746
CK1alpha can promote cell survival by interfering with RXR agonist-induced apoptosis PMID: 15131121
CK1alpha-mediated phosphorylation modulates the mRNA binding ability of hnRNP-C PMID: 15687492
NFAT1, a transcription factor connected with breast cancer metastasis, is activated by Wnt-5a through a Ca2+ signaling pathway in human breast epithelial cells which was simultaneously counteracted by a Wnt-5a-induced Yes/Cdc42 signaling pathway. PMID: 16880514
identification of the alpha isoform of casein kinase I as the cellular kinase important for the formation of hepatitis C virus (HCV) NS5A-p58; here we show the effects of this kinase on NS5A hyperphosphorylation and HCV replication PMID: 16943283
CK1alphaLS and hnRNP-C represent conserved components of a vertebrate-specific H(2)O(2)-responsive nuclear signaling pathway. PMID: 17626781
Studies suggest that CK1alpha is another kinase that regulates RIP1 function in NF-kappaB activation. PMID: 18067272
following DNA damage, PML facilitates Thr18 phosphorylation by recruiting p53 and CK1 into PML nuclear bodies, thereby protecting p53 from inhibition by Mdm2, leading to p53 activation. PMID: 18246126
Ser-155 and Ser-158 have important roles in the nucleocytoplasmic trafficking of OREBP/TonEBP and CK1 plays a major role in regulating this process PMID: 18411282
We show that the inhibition of CK1 activates RhoB and promotes RhoB dependent actin fiber formation and EGF-R level. PMID: 18590726
CK1 has a role as the Ser20 site kinase for p53 in DNA virus-infected cells PMID: 18669630
Phosphorylation of parkin by casein kinase I and cdk5 decreases parkin solubility, leading to its aggregation and inactivation. PMID: 19050041
'activated B-cell-like' subtype of diffuse large B-cell lymphoma cells required CK1alpha for constitutive NF-kappaB activity, indicating that CK1alpha functions as a conditionally essential malignancy gene PMID: 19118383
Casein Kinase 1 phosphorylates serine 56 of yellow fever virus methyltransferase. PMID: 19185594
Data show that Wnt-5a stimulation of breast epithelial cells leads to increased cell-cell adhesion, and that Wnt-5a/casein kinase Ialpha (CKIalpha)-specific phosphorylation of beta-catenin leads to increased complex formation of beta-catenin/E-cadherin. PMID: 19244247
CK1alpha plays a central role in mediating MDM2 control of p53 and E2F-1 protein stability PMID: 19759023
Results suggest a role for mammalian and parasite casein kinase 1alpha in regulating IFNAR1 stability and type I IFN signaling. PMID: 19805514

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