Name: Recombinant Human Carbonic Anhydrase IX / CA9 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-0557
Availability: InStock

Recombinant Human Carbonic Anhydrase IX / CA9 Protein (His Tag)

Catalog No.: BLPSN-0557

Product Overview

Tag	His
Host Species	Human
Accession	NP_001207.2
Synonym	CAIX, Carbonic Anhydrase IX, MN
Background	Carbonic anhydrases IX (CA IX), also known as membrane antigen MN or CA9, is a member of the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide (H2O + CO2 = H+ + HCO3-) and thus participate in a variety of biological and physical processes. CA IX protein is expressed primarily in carcinoma cells lines, and the expression is cell density dependent and has been shown to be strongly induced by hypoxia, accordingly facilitates adaptation of tumor cells to hypoxic conditions. It is involved in tumorigenesis through many pathways, such as pH regulation and cell adhesion control. CA IX is used as a marker of tumor hypoxia and as a new therapeutic target for many human carcinomas and cancers.Immune CheckpointImmunotherapyCancer ImmunotherapyTargeted Therapy
Description	A DNA sequence encoding the human carbonic anhydrase IX (CA9) precursor (NP_001207.2) (Met 1-Asp 414) was expressed with a His tag at the C-terminus.
Source	HEK293
Predicted N Terminal	Gln 38
AA Sequence	NP_001207.2
Molecular Weight	The recombinant human CA9 consists of 388 a.a. and predicts a molecular mass of 42.5 kDa. As a result of glycosylation, rhCA9 migrates with apparent molecular mass of 48 kDa in SDS-PAGE under reducing conditions.
Purity	>95% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Measured by its esterase activity. The specific activity is >30 pmoles/min/ug, as measured with 1 mM 4-Nitrophenyl acetate and 2.5 ug enzyme at 400 nm in 100 uL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.
Formulation	Lyophilized from sterile PBS, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.