Recombinant Human Carbonic Anhydrase IV / CA4 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-0552
Recombinant Human Carbonic Anhydrase IV / CA4 Protein (His Tag)

Recombinant Human Carbonic Anhydrase IV / CA4 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-0552
Catalog No.: BLPSN-0552

Product Overview

Tag His
Host Species Human
Accession NP_000708.1
Synonym CAIV, Car4, RP17
Background The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase IV (CAIV) is a membrane-associated enzyme anchored to plasma membrane surfaces by a phosphatidylinositol glycan linkage. CAIV is a high-activity isozyme in CO2 hydration comparable to that of CAII. Furthermore, CAIV is more active in HCO3- dehydration than is CAII. However, the esterase activity of CAIV is decreased 15-fold compared to CAII.
Description A DNA sequence encoding the human CA4 (NP_000708.1) (Met1-Lys283) without the pro peptide was expressed with a His tag at the C-terminus.
Source CHO Stable Cells
Predicted N Terminal Ala 19
AA Sequence Met1-Lys283
Molecular Weight The recombinant human CA4 consists of 276 a.a. after removal of the signal peptide and has a predicted molecular mass of 31.7 kDa. In SDS-PAGE under reducing conditions, rh CA4 migrates as approximately 34 and 19 kDa band.
Purity (48.3+49.3)% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Please contact us for detailed information
Formulation Lyophilized from sterile PBS, pH 7.4..
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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