Recombinant Human Carbonic Anhydrase IV / CA4 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-0551
Recombinant Human Carbonic Anhydrase IV / CA4 Protein (His Tag)

Recombinant Human Carbonic Anhydrase IV / CA4 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-0551
Catalog No.: BLPSN-0551

Product Overview

Tag His
Host Species Human
Accession NP_000708.1
Synonym CAIV, Car4, RP17
Background The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase IV (CAIV) is a membrane-associated enzyme anchored to plasma membrane surfaces by a phosphatidylinositol glycan linkage. CAIV is a high-activity isozyme in CO2 hydration comparable to that of CAII. Furthermore, CAIV is more active in HCO3- dehydration than is CAII. However, the esterase activity of CAIV is decreased 15-fold compared to CAII.
Description A DNA sequence encoding the human CA4 (NP_000708.1) (Met1-Lys283) without the pro peptide was expressed, fused with a His tag at the C-terminus.
Source HEK293
Predicted N Terminal Ala 19
AA Sequence Met1-Lys283
Molecular Weight The recombinant human CA4 consists of 276 a.a. after removal of the signal peptide and has a predicted molecular mass of 31.7 kDa. In SDS-PAGE under reducing conditions, rh CA4 migrates as an approximately 30 kDa band.
Purity >96% as determined by SDS-PAGE
Endotoxin < 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity Measured by its esterase activity. The specific activity is >2 pmoles/min/ug.
Formulation Lyophilized from sterile PBS, pH 7.4.
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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