Name: Recombinant Human CAR Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-0543
Availability: InStock

Catalog No.: BLPSN-0543

Product Overview

Tag	His
Host Species	Human
Accession	NP_001329.1
Synonym	CAR, CAR4/6, CXADR, HCAR
Background	CXADR (coxsackie virus and adenovirus receptor), also known as CAR, is a type I transmembrane glycoprotein belonging to the CTX family of the Ig superfamily, and is essential for normal cardiac development in the mouse. Proposed as a homophilic cell adhesion molecule, CXADR is a component of the epithelial apical junction complex that is essential for the tight junction integrity, and probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN). Mature mouse CXADR structrually comprises a 218 aa extracellular domain (ECD) with a V-type (D1) and a C2-type (D2) Ig-like domain, a 21 aa transmembrane segment and a 17 aa intracellular domain, among which,D1 is thought to be responsible for homodimer formation in trans within tight junctions. The ECD of mouse CXADR shares 97%, 9% sequence identity with the corresponding regions of rat, human CXADR.
Description	A DNA sequence encoding the human CXADR (NP_001329.1) extracellular domain (Met 1-Gly 237) was fused with a His tag at the C-terminus.
Source	HEK293
Predicted N Terminal	Leu 20
AA Sequence	Met 1-Gly 237
Molecular Weight	The secreted recombinant human CXADR consists of 229 a.a. and has a predicted molecular mass of 25.6 kDa. As a result of glycosylation, the apparent molecular mass of rh CXADR is approximately 35 kDa in SDS-PAGE under reducing conditions.
Purity	>92% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Measured by the ability of the immobilized protein to support the adhesion of mouse neutrophils. When 5 x 10E4 cells/well are added to CXADR coated plates (4 ug/ml and 100 ul/well), approximately 20%-40% will adhere specifically after 60 minutes at 37 °C.
Formulation	Lyophilized from sterile PBS, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.