Recombinant Human CAPG Protein

Name: Recombinant Human CAPG Protein
Brand: Beta LifeScience
SKU: BLPSN-0541
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Tag	N/A
Host Species	Human
Accession	AAH14549.1
Synonym	AFCP, HEL-S-66, MCP
Background	CAPG, also known as actin Regulatory Protein CAPG, is a a member of the gelsolin/villin family. Members of this family are actin-regulatory proteins. CAPG reversibly blocks the barbed ends of F-actin filaments in a Ca2+ and phosphoinositide-regulated manner, but does not sever preformed actin filaments. By capping the barbed ends of actin filaments, CAPG contributes to the control of actin-based motility in non-muscle cells. CAPG may also play an important role in macrophage function.
Description	A DNA sequence encoding human CAPG(AAH14549.1) (Met1-Lys348) was expressed.
Source	E.coli
Predicted N Terminal	Met
AA Sequence	Met1-Lys348
Molecular Weight	The recombinant human CAPG consists of 348 a.a. and predicts a molecular mass of 38.5 KDa. It migrates as an approximately 43 KDa band in SDS-PAGE under reducing conditions.
Purity	>95% as determined by SDS-PAGE
Endotoxin	Please contact us for more information.
Bioactivity	Please contact us for detailed information
Formulation	Lyophilized from sterile 20mM tris, 10% glycerol, pH 8.0..
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA.
Subcellular Location	Nucleus. Cytoplasm. Melanosome. Cell projection, lamellipodium. Cell projection, ruffle.
Protein Families	Villin/gelsolin family
Database References	HGNC: 1474 OMIM: 153615 KEGG: hsa:822 STRING: 9606.ENSP00000263867 UniGene: Hs.516155
Tissue Specificity	Macrophages and macrophage-like cells.

Gene Functions References

Study suggested that CapG could be used as a biomarker for metastatic CRC in the clinical specimens. Moreover, our in vitro study demonstrated that CapG might contribute on tumor metastasis in human CRCs. PMID: 30155403
Results revealed that CapG is a novel independent prognostic predictor for glioma patients and highlight a key role of CapG in proliferation and metastasis of glioma. PMID: 29399702
it is demonstrated that CapG is expressed in the cytoplasm and could be used as a prognostic or diagnostic biomarker for mHCC in clinical specimens PMID: 29970516
The combination of CTNB1, XPO2, and CAPG achieved 95% sensitivity and 96% specificity for the discrimination of these subtypes. We developed two uterine aspirate-based signatures to diagnose Endometrial cancer and classify tumors in the most prevalent histologic subtypes. This will improve diagnosis and assist in the prediction of the optimal surgical treatment PMID: 28790116
Overexpression of CAPG is associated with glioma. PMID: 26663173
On the basis of these results, we propose a model in which dynamic vimentin filaments target CARMIL2 to critical membrane-associated locations, where CARMIL2 regulates CP, and thus actin assembly, to create cell protrusions PMID: 26466680
The composite biomarker, CAPG and GIPC1 in primary breast tumors, predicted disease outcomes and benefit from zoledronate and may facilitate patient selection for adjuvant bisphosphonate treatment. PMID: 26757732
A single nucleotide polymorphism rs6886 inside the CapG gene was identified, affecting a CapG phosphorylation site and thus potentially modifying CapG function. PMID: 24804218
CapG was up-regulated in the tumor tissues of patients with lymph node metastasis (LNM), whereas it showed an equivalent expression level between non-tumor and tumor tissues of patients without LNM. PMID: 23782053
CapG is involved in the process of metastasis by promoting the invasiveness of tumor cells. PMID: 23085225
CapG was identified as a novel candidate biomarker to predict response to gemcitabine treatment and survival in cholangiocarcinoma. PMID: 22155129
Expression of CapG, gelsolin, and P-gp was found to be associated with an increased risk of death from non-small cell lung cancer. PMID: 22190510
These results suggest that overexpression of CapG may be associated with progression of lung adenocarcinoma. PMID: 21908955
strong down-regulation of MCK activity contributes to F-actin instability and induces post-translational modification of alphaB-crystallin and desmin PMID: 21768101
CapG was upregulated in the stroma cells of nasopharyngeal carcinoma (NPC) compared with normal nasopharyngeal epithelial tissues. CapG possibly plays a role in the complex interaction between NPC cells and the surrounding host tissue. PMID: 19242827
Proteomic study of macrophages exposed to oxLDL identifies a CAPG polymorphism associated with carotid atherosclerosis. PMID: 19439302
CapG lacks a nuclear export sequence present in structurally related proteins PMID: 12637565
importin-beta-dependent nuclear import of the actin modulating protein CapG promotes cell invasion PMID: 15454578
CapG is a new tumor suppressor gene involved in the tumorigenic progression of certain cancers PMID: 16767159
Comprehension of the mobility and compartmentalization of the CapG protein in normal and in cancer cells in vivo could constitute a new basis to characterize the invasiveness and metastasizing potential of breast cancer. PMID: 18059028
Dysregulated expression of gelsolin-like actin capping protein(CapG) was found in premalignant and malignant oral carcinogenesis. PMID: 18237446
Results report that NTF2 and Ran control nuclear import of the filamentous actin capping protein CapG. PMID: 18266911
CapG mobility in cell nuclei of live breast cancer cells PMID: 18461482
the 4 biomarkers CLU, ITGB3, PRAME and CAPG may be used as prognostic factors for patients with stage III serous ovarian adenocarcinomas. PMID: 18709641
These results suggest that filamentous actin in the nucleolus might be regulated by actin binding proteins such as CapG. PMID: 18938132
several genes that are known to be regulated by DNA methylation were up-regulated dramatically by integrin alpha6beta4 expression, including S100A4, FST, PDLIM4, CAPG, and Nkx2.2. PMID: 19011242
A role for the actin-binding protein CapG as a mediator of cross-talk between the actin cytoskeleton and microtubule-based organelles that regulate cell division was proposed. PMID: 19166812
Hypoxia-inducible factor 1alpha up-regulated CapG protein expression under normoxia. Knockdown of HIF-1alpha expression in hPASMCs also inhibited hypoxia-induced CapG up-regulation. PMID: 19188659

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.