Recombinant Human CaMKII alpha Protein (GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0533
Recombinant Human CaMKII alpha Protein (GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0533
Catalog No.: BLPSN-0533
Product Overview
| Tag | GST |
| Host Species | Human |
| Accession | NP_741960.1 |
| Synonym | CAMKA |
| Background | Ca2+/calmodulin-dependent protein kinase2A (CAMK2A) belongs to the serine/threonine protein kinase family and, together with other 28 different isoforms, belongs to the Ca2+/ calmodulin-dependent protein kinase subfamily. CaM kinase -…¡ is thought to be an important mediator of learning and memory and is also necessary for Ca2+ homeostasis and reuptake in cardiomyocytes chloride transport in epithelia, positive T-cell selection, and CD8 T-cell activation. CAMKIIA is one of the major forms of CAMKII. It has been found to play a critical role in sustaining activation of CAMKII at the postsynaptic density. Studies have found that knockout mice without CAMKIIA demonstrate a low frequency of LTP. Additionally, these mice do not form persistent, stable place cells in the hippocampus. |
| Description | A DNA sequence encoding the human CAMK2A isoform 2 (NP_741960.1) (Met 1-His 478) was fused with the GST tag at the N-terminus. |
| Source | Baculovirus-Insect Cells |
| Predicted N Terminal | Met |
| AA Sequence | Met 1-His 478 |
| Molecular Weight | The recombinant human CAMK2A/GST chimera consists of 703 a.a. and predicts a molecular mass of 80.3 kDa. It migrates as an approximately 80 kDa band in SDS-PAGE under reducing conditions. |
| Purity | >85% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | The specific activity was determined to be 160 nmol/min/mg using Autocamtide-2 synthetic peptide (KKALRRQETVDAL-amide) as substrate. |
| Formulation | Supplied as sterile 50mM Tris, 100mM NaCl, 0.5mM PMSF, 0.5mM Reduced Glutathione, pH 8.0. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
