Recombinant Human Calpain Small Subunit 1 (CAPNS1) Protein (His-SUMO)

Name: Recombinant Human Calpain Small Subunit 1 (CAPNS1) Protein (His-SUMO)
Brand: Beta LifeScience
SKU: BLC-03952P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Calpain Small Subunit 1 (CAPNS1) Protein (His-SUMO) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P04632
Target Symbol	CAPNS1
Synonyms	30K; Calcium activated neutral proteinase; Calcium activated neutral proteinase small subunit; Calcium dependent protease small subunit 1; Calcium dependent protease small subunit; Calcium-activated neutral proteinase small subunit; Calcium-dependent protease small subunit 1; Calcium-dependent protease small subunit; Calpain 4; Calpain 4 small subunit (30K); Calpain 4 small subunit; Calpain regulatory subunit; Calpain S1; Calpain small polypeptide; Calpain small subunit 1; Calpain4; CalpainS1; CANP; CANP small subunit; CANPS; CAPN 4; CAPN S1; CAPN4; CAPNS 1; CAPNS; Capns1; CDPS; CPNS1_HUMAN; CSS 1; CSS1
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His-SUMO
Target Protein Sequence	MFLVNSFLKGGGGGGGGGGGLGGGLGNVLGGLISGAGGGGGGGGGGGGGGGGGGGGTAMRILGGVISAISEAAAQYNPEPPPPRTHYSNIEANESEEVRQFRRLFAQLAGDDMEVSATELMNILNKVVTRHPDLKTDGFGIDTCRSMVAVMDSDTTGKLGFEEFKYLWNNIKRWQAIYKQFDTDRSGTICSSELPGAFEAAGFHLNEHLYNMIIRRYSDESGNMDFDNFISCLVRLDAMFRAFKSLDKDGTGQIQVNIQEWLQLTMYS
Expression Range	1-268aa
Protein Length	Full Length
Mol. Weight	44.3kDa
Research Area	Signal Transduction
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
Subcellular Location	Cytoplasm. Cell membrane.
Database References	HGNC: 1481 OMIM: 114170 KEGG: hsa:826 STRING: 9606.ENSP00000246533 UniGene: PMID: 29331389 Calpain small subunit 1 (Capn4) overexpression increased the protein level of cleaved talin and and activated the focal adhesion kinase (FAK)/AKT/MAPK signaling in 786-O cells, while Capn4 silencing decreased the protein level of cleaved talin in Caki-1 cells. PMID: 29648579 Capn4 and miR-1271 may serve as potential therapeutic targets for the treatment of colorectal cancer. PMID: 29331762 MALAT1 and Capn4 were up-regulated while miR-124 expression was down-regulated in nasopharyngeal carcinoma cell lines. PMID: 28857668 Capn4 promotes epithelial-mesenchymal transition in human melanoma cells through activation of the Wnt/beta-catenin pathway. PMID: 27878263 CAPNS1 depletion is coupled to impairment of breast cancer cell growth. PMID: 26771715 High levels of Capn4 expression were associated with lymph node metastasis and large tumor size in non-small cell lung cancer patients.Capn4 promotes non-small cell lung cancer progression via upregulation of matrix metalloproteinase 2. PMID: 25636510 HBXIP was able to up-regulate Capn4 at the levels of promoter. PMID: 25304384 The overexpression of Capn4 is a novel negative prognostic marker, and Capn4 may be used as a new target in therapeutic strategies for human glioma. PMID: 24628706 Capn4 can contribute to hepatocellular carcinoma growth and metastasis PMID: 24962955 Capn4 mRNA and protein are increased in human clear cell renal cell carcinoma tissues and higher Capn4 levels are associated with advanced clinical tumour stage and shorter overall survival. PMID: 24514433 Capn4 upregulated MMP2 via nuclear factor-kappaB activation, manifested by increased phosphorylation of p65, a subunit of NF-kappaB. PMID: 24703594 This study suggests that augmented calpain activity is associated with increased incidence of thrombosis under hypoxic environments. PMID: 24297866 Capn4 overexpression was implicated in ICC metastasis/invasion, and Capn4 overexpression may be used as a molecular therapeutic target for intrahepatic cholangiocarcinoma PMID: 23349941 The authors conclude that hepatitis B virus X protein upregulate Capn4 through NF-kappaB/p65 to promote migration of hepatoma cells. PMID: 20419804 CAPNS1 depletion is coupled to reduced levels of H2AX phosphorylation, not only in Ras(v12) induced BJ-ET fibroblasts, but also in a number of cellular systems upon genotoxic stress PMID: 20107320 Genetic variations in LEP, CAPNS1 and ALOX15 show significant association with IL-6 expression. PMID: 19942621 calpain-mediated XIAP degradation contributes to initiation of apoptosis in normal neutrophils and dysfunction of this regulatory pathway can lead to pathological neutrophil accumulation. PMID: 12121983 Whereas alphaPIX guanine nucleotide exchange factor activity contributes to enhanced formation of cellular protrusions, the GEF-independent association with calpain 4 leads to induction of a yet unknown signaling cascade resulting in cell spreading. PMID: 15611136 results reveal that especially NRF-1, along with AP-1 and, to a minor extent, an Sp1 site, is essential for human CAPNS1 promoter activity and gene expression PMID: 18234454 c-Myc regulates calpain activity through calpastatin; apoptosis induced by calpain inhibition is dependent on c-Myc, and calpastatin knockdown promotes transformation in c-Myc-negative cells PMID: 18544539 RasGAP and Capns1 interaction in oncogenic Ras cells is involved in regulating migration and cell survival. PMID: 18761085

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.