Recombinant Human CALML3 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0522

Recombinant Human CALML3 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0522
Catalog No.: BLPSN-0522
Product Overview
Tag | His |
Host Species | Human |
Accession | P27482 |
Synonym | CLP |
Background | Calmodulin-like protein 3 (CALML3) is similar to that of authentic calmodulin and may actually compete with calmodulin by binding, with different affinity, to cellular substrates. Calmodulin-like protein 3 (CALML3) is a tumor-sensitive protein specifically expressed in normal epithelial cells but downregulated in tumorigenesis. Downregulation of the protein is an early event in breast cancer development. One of the most pressing questions raised by the discovery of CLP/CALML3 is that of its potential targets. Although it is 85% identical to human calmodulin, the distinct properties of CLP suggest that it has specific targets or targets that only partially overlap with those of calmodulin. Research has identified the unconventional myosin-1 (Myo1) as a specific target of CALML3. The discovery of Myo1 as a specific target of CALML3 is highly significant and suggests multiple lines of further research such as investigations of the Ca2+ regulation of Myo1 and the role of the loss of CLP in epithelial differentiation, adhesion, and cancer. Cells expressing CALML3 displayed a striking increase in the number and length of myosin-1-positive filopodia and showed increased mobility in a wound healing assay. |
Description | A DNA sequence encoding the human CALML3 (P27482) (Met 1-Lys 149) was expressed, with a His tag at the N-terminus. |
Source | E.coli |
Predicted N Terminal | Met |
AA Sequence | Met 1-Lys 149 |
Molecular Weight | The recombinant human CALML3 consisting of 164 a.a. and has a calculated molecular mass of 18.7KDa. It migrates as an 16 kDa band in SDS-PAGE under reducing conditions. |
Purity | >95% as determined by SDS-PAGE |
Endotoxin | Please contact us for more information. |
Bioactivity | Please contact us for detailed information |
Formulation | Lyophilized from sterile PBS, pH 7.4. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |