Name: Recombinant Human Calcineurin B / CALNB1 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-0518
Availability: InStock

Recombinant Human Calcineurin B / CALNB1 Protein (His Tag)

Catalog No.: BLPSN-0518

Product Overview

Tag	His
Host Species	Human
Accession	P63098
Synonym	CALNB1, CNB, CNB1
Background	PPP3R1 belongs to the calcineurin regulatory subunit family. It is a regulatory subunit of calcineurin. Calcineurin is composed of two subunits: calcineurin A (CnA) and calcineurin B (CnB). Dephosphorylation of the nuclear factor of activated T-cells (NF-AT) by Calcineurin is essential for NF-AT activation, nuclear translocation, and early gene expression in T-cells. PPP3R1 is a Ser/Thr-specific calcium and calmodulin-dependent protein phosphatase which takes a vital part in the T cell activation pathway. PPP3R1 is involved in protein dephosphorylation, NFAT protein import into nucleus (ortholog) and epithelial to mesenchymal transition (ortholog). It participates in calcineurin signaling pathway; mitogen activated protein kinase signaling pathway. PPP3R1 interacts with (+)-pilocarpine, 2,4-dinitrotoluene and ammonium chloride. It contains four EF-hand domains and four functional calcium-binding sites. PPP3R1 play an improtant role in the T cell activation pathway.
Description	A DNA sequence encoding the human PPP3R1 (P63098) (Gly2-Val170) was fused with a His tag at the N-terminus.
Source	Baculovirus-Insect Cells
Predicted N Terminal	His
AA Sequence	Gly2-Val170
Molecular Weight	The recombinant human PPP3R1 consists of 187 a.a. and has a calculated molecular mass of 21.4 kDa. The recombinant protein migrates as an approximately 20 kDa band in SDS-PAGE under reducing conditions.
Purity	>87% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Using the Octet RED System, the affinity constant (Kd) of human PPP3R1-His bound to Human PPIA-His was 6 nM.
Formulation	Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0, 10% glycerol, 2mM DTT.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.