Recombinant Human Calcineurin A / CALNA1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0517
Recombinant Human Calcineurin A / CALNA1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0517
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Human |
Accession | Q08209 |
Synonym | CALN, CALNA, CALNA1, CCN1, CNA1, PPP2B |
Background | PPP3CA, also known as protein phosphatase 2B, is a member of the PPP phosphatase family, PP-2B subfamily. It is the alpha catalytic subunit of protein phosphatase 2B (PP2B). PP2B is a holoenzyme that is comprised of a catalytic subunit associated with regulatory subunits. It is a calcium regulated enzyme that is activated by calmodulin and participates in the signaling cascades involved in development of the nervous, cardiovascular, and musculoskeletal systems. PPP3CA activates the T cells of the immune system and can be blocked by drugs. It also activates NFATc (a transcription factor) by dephosphorylating it. The activated NFATc is subsequently translocated into the nucleus, where it upregulates the expression of interleukin 2. PPP3CA interacts with CRTC2, MYOZ1, MYOZ2 and MYOZ3. It also interacts with DNM1L. The interaction dephosphorylates DNM1L and regulates its translocation to mitochondria. |
Description | A DNA sequence encoding the human PPP3CA (Q08209-1) (Ser2-Gln521) was fused with a His tag at the N-terminus. |
Source | Baculovirus-Insect Cells |
Predicted N Terminal | His |
AA Sequence | Ser2-Gln521 |
Molecular Weight | The recombinant human PPP3CA consists of 538 a.a. and has a calculated molecular mass of 60.8 kDa. The recombinant protein migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions. |
Purity | >94% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Using the Octet RED System, the affinity constant (Kd) of human PPPP3CA-His bound to Human PPIA-His was 0.9 nM. |
Formulation | Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% glycerol. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |