Recombinant Human CADM1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0506
Recombinant Human CADM1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0506
Catalog No.: BLPSN-0506
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | NP_055148.3 |
| Synonym | BL2, IGSF4, IGSF4A, Necl-2, NECL2, RA175, sgIGSF, ST17, sTSLC-1, SYNCAM, synCAM1, TSLC1 |
| Background | Members of the immunoglobulin superfamily often play key roles in intercellular adhesion. IGSF4 is a novel immunoglobulin (Ig)-like intercellular adhesion molecule. Three Ig-like domains are included in the extracellular domain of IGSF4 and mediate homophilic or heterophilic interactions independently of Ca2+. The cytoplasmic domain of IGSF4 contains the binding motifs that connect to actin fibers. Since IGSF4 has been characterized by several independent research groups, this molecule is called by three names, TSLC1, SgIGSF and SynCAM. IGSF4 was first characterized as a tumor suppressor of non-small cell lung cancer and termed TSLC1. It is a single-pass type I membrane protein which belongs to the nectin family, which may be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. In addition, CADM1 may play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa. In neuroblastoma, loss of CADM1 expression has recently been found in disseminated tumours with adverse outcome, prompting us to investigate its role in neuroblastoma tumour progression. The downregulation of CADM1 tumour suppressor gene expression is a critical event in neuroblastoma pathogenesis resulting in tumour progression. |
| Description | A DNA sequence encoding the human CADM1 (NP_055148.3) extracellular domain (Met 1-His 374) was expressed, fused with a His tag at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Gln 45 |
| AA Sequence | Met 1-His 374 |
| Molecular Weight | The secreted recombinant human CADM1 consists of 341 a.a. after removal of the signal peptide and has a predicted molecular mass of 38.5 kDa. As a result of glycosylation, the apparent molecular masss of rh CADM1 is approximately 70-80 kDa in SDS-PAGE under reducing conditions. |
| Purity | >94% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by its binding ability in a functional ELISA. Immobilized recombinant human CADM1 at 2 ug/ml (100 ul/well) can bind biotinylated human CRTAM with a linear range of 12.5-400 ng/ml. |
| Formulation | Lyophilized from sterile PBS, pH 7.4. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
