Recombinant Human CA5A Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0495
Recombinant Human CA5A Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0495
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Human |
Accession | NP_001730.1 |
Synonym | CA5, CA5AD, CA5D, Carbonic Anhydrase VA, CAV, CAVA, GS1-21A4.1 |
Background | Carbonic anhydrase 5A, mitochondrial, also known as Carbonate dehydratase VA, Carbonic anhydrase VA, CA-VA and CA5A, is a member of thealpha-carbonic anhydrase family. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA5A / CA-VA is activated by histamine, L-adrenaline, L- and D-histidine, and L- and D-phenylalanine. It is inhibited by coumarins, sulfonamide derivatives such as acetazolamide and Foscarnet (phosphonoformate trisodium salt). |
Description | A DNA sequence encoding the mature form of human CA5A (NP_001730.1) (Ala 40-Ser 305) was fused with an Met at N-terminus and a His tag at the C-terminus. |
Source | E.coli |
Predicted N Terminal | Met |
AA Sequence | Ala 40-Ser 305 |
Molecular Weight | The recombinant human CA5A consisting of 277 a.a. and has a calculated molecular mass of 31.6 kDa. It migrates as an approximately 33 kDa band in SDS-PAGE under reducing conditions. |
Purity | >96% as determined by SDS-PAGE |
Endotoxin | Please contact us for more information. |
Bioactivity | Measured by its esterase activity. The specific activity is >500 pmoles/min/ug. |
Formulation | Lyophilized from sterile 50mM NaAc, 50mM NaCl, 0.05% Brij 35, pH 5.0. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |