Recombinant Human CA12 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0492
Recombinant Human CA12 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0492
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Human |
Accession | NP_001209.1 |
Synonym | CA12, CAXII, FLJ20151, HsT18816 |
Background | Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA12, also known as Car12 and carbonic anhydrase XII, is a type I membrane enzyme of an N-terminal extracellular catalytic domain, a membrane-spanning alpha-helix, and a small intracellular C-terminal domain. It is highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis. Overexpression of the CA12 is observed in certain human cancers and is used as a tumor marker. rmCA12 corresponds to the extracellular domain and has both carbonic anhydrase activity and esterase activity. |
Description | A DNA sequence encoding the extracellular domain of human CA12 (NP_001209.1) (Met 1-Gln 291) was expressed with a C-terminal His tag. |
Source | HEK293 |
Predicted N Terminal | Ala 25 |
AA Sequence | Met 1-Gln 291 |
Molecular Weight | The recombinant human CA12 consisting of 278 a.a. and has a calculated molecular mass of 31.6 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 40-45 kDa protein in SDS-PAGE under reducing conditions. |
Purity | >97% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | Measured by its esterase activity. The specific activity is >40 pmoles/min/ug, as measured with 1 mM 4-Nitrophenyl acetate and 2.5 ug enzyme at 400 nm in 100 uL of 12.5 mM Tris, 75 mM NaCl, pH 7.5. |
Formulation | Lyophilized from sterile PBS, pH 7.4. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |