Recombinant Human c-MET Protein (aa 956-1390, His & GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1338
Recombinant Human c-MET Protein (aa 956-1390, His & GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-1338
Catalog No.: BLPSN-1338
Product Overview
| Tag | His&GST |
| Host Species | Human |
| Accession | P08581 |
| Synonym | AUTS9, c-Met, DFNB97, HGFR, RCCP2 |
| Background | Hepatocyte growth factor receptor (HGFR), also known as c-Met or mesenchymal-epithelial transition factor (MET), is a receptor tyrosine kinase (RTK) that has been shown to be overexpressed and/or mutated in a variety of malignancies. HGFR protein is produced as a single-chain precursor, and HGF is the only known ligand. Normal HGF/HGFR signaling is essential for embryonic development, tissue repair or wound healing, whereas aberrantly active HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. HGFR protein is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. Preclinical studies suggest that targeting aberrant HGFR signaling could be an attractive therapy in cancer.Immune CheckpointImmunotherapyCancer ImmunotherapyTargeted Therapy |
| Description | A DNA sequence encoding the human MET (P08581-1) (Lys956-Ser1390) was fused with the N-terminal His-tagged GST tag at the N-terminus. |
| Source | Baculovirus-Insect Cells |
| Predicted N Terminal | Met |
| AA Sequence | Lys956-Ser1390 |
| Molecular Weight | The recombinant human MET /GST chimera consists of 672 a.a. and has a calculated molecular mass of 76.8 kDa. The recombinant protein migrates as an approximately 68 kDa band in SDS-PAGE under reducing conditions. |
| Purity | >90% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | 1. The specific activity was determined to be 10 nmol/min/mg using MBP as substrate.2. Measured by its binding ability in a functional ELISA. Immobilized human HGFR (aa 956-1390) at 10 ug/ml (100 ul/well) can bind biotinylated human HGF-his with a linear range of 15.6-125 ng/ml. |
| Formulation | Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol, 3mM DTT. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
