Recombinant Human C-C Motif Chemokine 7 Protein (CCL7), Active

Name: Recombinant Human C-C Motif Chemokine 7 Protein (CCL7), Active
Brand: Beta LifeScience
SKU: BLC-05512P
Availability: InStock

Collections: All products, Buy cytokines, chemokines, and growth factors for research online, Chemokines and receptors – essential regulators of immune response, High-quality cytokines for advanced research, High-quality recombinant proteins

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

Submit an inquiry today to inquire about all available size options and prices! Connect with us via the live chat in the bottom corner to receive immediate assistance.

Product Overview

Description	Recombinant Human C-C Motif Chemokine 7 Protein (CCL7), Active is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 97% as determined by SDS-PAGE and HPLC.
Endotoxin	Less than 1.0 EU/μg as determined by LAL method.
Activity	Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/ml.
Uniprotkb	P80098
Target Symbol	CCL7
Synonyms	C-C motif chemokine 7; Ccl7; CCL7_HUMAN; Chemokine CC motif ligand 7; FIC; MARC; MCP-3; Monocyte chemoattractant protein 3; Monocyte chemotactic protein 3; NC28; RP23-350G1.4; SCYA6; SCYA7; Small-inducible cytokine A7
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	Tag-Free
Complete Sequence	QPVGINTSTT CCYRFINKKI PKQRLESYRR TTSSHCPREA VIFKTKLDKE ICADPTQKWV QDFMKHLDKK TQTPKL
Expression Range	24-99aa
Protein Length	Full Length of Mature Protein
Mol. Weight	9.0 kDa
Research Area	Immunology
Form	Lyophilized powder
Buffer	Lyophilized from a 0.2 μm filtered 20 mM PB, pH 7.4, 150 mM NaCl
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3.
Subcellular Location	Secreted.
Protein Families	Intercrine beta (chemokine CC) family
Database References	HGNC: 10634 OMIM: 158106 KEGG: hsa:6354 STRING: 9606.ENSP00000367832 UniGene: PMID: 28475003 Periprostatic adipocytes drive prostate cancer progression in obesity via CCL7 secretion which stimulates CCR3 expressing tumor cells. PMID: 26756352 These results suggest that let-7d may suppress renal cell carcinoma growth, metastasis, and tumor macrophage infiltration at least partially through targeting COL3A1 and CCL7. PMID: 25193015 Based on nasopharyngeal CCL7 gene expression in readily available Nasopharyngeal aspirate samples , we can discriminate between severity of disease in Respiratory syncytial virus infected infants. PMID: 25261323 Overexpression of ISL1 in human mesnchymal stem cells promotes angiogenesis in vitro and in vivo through increasing secretion of MCP3 and other paracrine factors. PMID: 24578274 Our data demonstrate that the monocyte-specific chemokine CCL7 and its receptor CCR2 are expressed in tumour cells of RCC PMID: 24327013 Systemic IFN-gamma triggered the secretion of C-C motif ligand chemokines CCL2 and CCL7 leading to the egress of early, myeloid-committed progenitors from the bone marrow mediated by their common receptor CCR2. PMID: 23762028 Anticancer effect of desmethyl-lasiodiplodin is mediated, in part, by upregulation of apoptotic genes and downregulation of MCP-3. PMID: 23764760 Immunohistochemistry and real-time PCR analysis showed that CCL7 was expressed in normal colonic epithelium and the expression was higher in liver metastases compared to primary CRC. PMID: 22614322 These results suggest that overexpression of CCL21 and CCL7 is associated with tumor metastasis and serves as a prognostic factor in patients with gastric cancer. PMID: 22468089 MCP-3 is produced by human coronary artery smooth muscle cells and directly induces CASMC proliferation in vitro, suggesting a potential role for this chemokine in vascular pathology PMID: 21536288 we established, for the first time, a significant association of MCP3 variants with atopic asthma. PMID: 21388664 Basal monocyte migration may be facilitated by the astrocyte-derived cytokine CCL7 whose production is rapidly increased by TNF-alpha and thus likely plays a critical role in initiating neuroinvasion by SIV/HIV. PMID: 21279498 The expression of MCP-3 in laryngeal squamous cell carcinoma is much higher than in normal tissue. PMID: 18533557 IL-12 p35, ICAM-1 and MCP-3 mRNAs are expressed in primary nasal epithelial cells. PMID: 19253530 Cytoplasmic GR interacts with a subset of CCL7 mRNA through specific sequences and can regulate turnover rates, a novel posttranscriptional role for GR as an RNA-binding protein. PMID: 21148795 Monocyte chemoattractant protein-3 (MCP-3) was highly expressed in patients with chronic periodontitis, particularly in those with progressive periodontal lesions. PMID: 20151806 higher expression in the genital mucosa than in the blood of HIV-1-infected women from Benin PMID: 19898927 Elevated level of CCL7 is associated with invasion and migration of oral squamous cell carcinoma. PMID: 19937793 The two most abundant alleles of the CA/GA microsatellite polymorphism in the promoter-enhancer region of the MCP-3 gene, A2 and A3, are significantly associated with multiple sclerosis in the Belgian case-control study, but not in the family study. PMID: 12127674 overexpression of MCP-3 in early-stage systemic sclerosis suggests a novel role for this protein as a fibrotic mediator activating extracellular matrix gene expression in addition to promoting leukocyte trafficking. PMID: 12847692 monocyte chemotactic protein-3 uses a different mode of action from eotaxin PMID: 14733956 data suggest that cyclic mechanical stretch of the uterine cervix by the presenting part of the fetus during labour may augment both IL-8 and MCP-3 production in the uterine cervix via AP-1 activation. PMID: 15194816 These results suggest that oxLDL delivers its signal for MCP-3 expression via PPARgamma, which may be further related to the atherogenesis. PMID: 15381085 A non-heparin-binding mutant CCL7 inhibits chemotactic potential of synovial fluid from patients with active rheumatoid arthritis and demonstrates the glycosaminoglycan-binding requirement for chemokine-driven inflammation in vivo PMID: 16002730 CCR8 ligands are allotropic, binding to distinct sites within CCR8; the human immune system may have evolved to use CCL7 as a selective antagonist of viral chemokine activity at CCR8 but not those of the host ligand PMID: 17023422 MCP-3 is critical for monocyte mobilization and suggests new roles for monocyte chemoattractants in leukocyte homeostasis. PMID: 17364026 CCL7 synergizes with coproduced CXCL8 in peripheral blood monocyte migration. PMID: 18469140 increased plasma levels in patients with amnestic mild cognitive impairment PMID: 19403065 High MCP-3 is associated with macrophage/microglia infiltration in gliomas. PMID: 19424580

FAQs

Please fill out the Online Inquiry form located on the product page. Key product information has been pre-populated. You may also email your questions and inquiry requests to sales1@betalifesci.com. We will do our best to get back to you within 4 business hours.

Feel free to use the Chat function to initiate a live chat. Our customer representative can provide you with a quote immediately.

Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.